[PDF] Top 20 Development of automated brightfield double In Situ hybridization (BDISH) application for HER2 g ene and chromosome 17 centromere (CEN 17) for breast carcinomas and an assay performance comparison to manual dual color HER2 fluorescence In Situ hybridization (FISH)
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Development of automated brightfield double In Situ hybridization (BDISH) application for HER2 g ene and chromosome 17 centromere (CEN 17) for breast carcinomas and an assay performance comparison to manual dual color HER2 fluorescence In Situ hybridization (FISH)
... polysomy and no HER2 gene amplification have better prognosis compared to patients with HER2 gene amplifi- cation [11]. Dual color FISH for HER2 and CEN 17 targets is recommended especially for borderline IHC cases [12]. ... See full document
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Development of rRNA Targeted PCR and In Situ Hybridization with Fluorescently Labelled Oligonucleotides for Detection of Yersinia Species
... FIG. 4. Detection of Yersinia species by in situ hybridization. Bars, 10 mm. Binding of at least two differently labelled probes results in distinct mixed colors, as shown in the additive-color illustration ... See full document
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Development of an Improved Method of Mitotic Metaphase Chromosome Preparation Compatible for Fluorescence in situ Hybridization in Cotton
... In situ Hybridization (FISH) and Genomic In situ Hybridization (GISH), are excellent tools to study the structure and func- tion of chromosomes, polyploidy, aneuploidy, alien gene ... See full document
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Clinical application of genetic testing for posterior uveal melanoma
... rapid development of new molecular tech- niques that allow for examination of the genetic make-up of tumor cells, more accurate prognostic information has become available to clinicians and ...in situ ... See full document
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Characterization of mouse parvovirus infection by in situ hybridization.
... in situ hybridization (ISH) studies, using infant euthymic mice and adult scid mice, indi- cated that MPV-1 infection is lymphocytotropic in infant and adult mice and demonstrated viral DNA in scid mouse ... See full document
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Dealing with the problem of non-specific in situ mRNA hybridization signals associated with plant tissues undergoing programmed cell death
... Background: In situ hybridization is a general molecular method typically used for the localization of mRNA transcripts in plants. The method provides a valuable tool to unravel the connection between gene ... See full document
9
A rhesus monkey model to characterize the role of gastrin releasing peptide (GRP) in lung development Evidence for stimulation of airway growth
... Gastrin-releasing peptide (GRP) is developmentally expressed in human fetal lung and is a growth factor for normal and neoplastic lung but its role in normal lung development has yet to be clearly defined. In this ... See full document
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A novel ViewRNA in situ hybridization method for the detection of the dynamic distribution of Classical Swine Fever Virus RNA in PK15 cells
... the development of novel RNA in situ Hybridization method, quantitatively localization and visualization of the virus RNA molecular in cultured cell or tissue section becomes very important tool to ... See full document
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CALIBRATION OF DENSITOMETRY IN RADIO-ISOTOPIC IN SITU HYBRIDIZATION
... Various series of calibration spots (Table 1) were measured to determine the influence of emulsion, exposure time and development time on the relation between OD and radioactivity. For all series the correlation ... See full document
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Myoinhibitory peptide regulates feeding in the marine annelid Platynereis
... in situ hybridization showed that MIP is expressed during both larval and postlarval development and continues to be expressed after cephalic metamorphosis, in the early adult stage (Figure 1A-C, ... See full document
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Neuropathology for the Neuroradiologist: Fluorescence in Situ Hybridization
... With the knowledge of DNA and chromosomes, scientists began to unravel the mystery of heredity and applied the re- sulting discoveries to the understanding of such disciplines as tumor development. Specific ... See full document
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Interpretation of Mutations, Expression, Copy Number in Somatic Breast Cancer: Implications for Metastasis and Chemotherapy
... the development and application of genome-wide ...in-situ hybridization (FISH), array comparative genomic hybridization (aCGH), and next generation DNA- and RNA-sequencing, to improve upon ... See full document
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Is sperm FISH analysis still useful for Robertsonian translocations? Meiotic analysis for 23 patients and review of the literature
... sperm. Development of fluores- cence in-situ hybridization (FISH) technique has simpli- fied the analysis of sperm chromosomal content and has enabled to collect numerous data on meiotic segregation ... See full document
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A potential role for protease nexin 1 overexpression in the pathogenesis of scleroderma
... In situ hybridization of skin biopsy specimens demonstrated PN1 expression in the dermis of four out of six scleroderma patients but no PN1 expression in the dermis of six healthy ...the development ... See full document
13
Characterization of Chromosomal Abnormalities in Cancer by Spectral Karyotyping
... the development of fluorescent in situ hybridization technique (FISH) enabled detection of chromosomal microdeletions and structural abnormalities ...genomic hybridization (17) and spectral ... See full document
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Transient elevation of messenger RNA encoding gastrin releasing peptide, a putative pulmonary growth factor in human fetal lung
... pulmonary development (from approximately 16 to 30 wk ...in situ hybridization analyses, GRP mRNAs were first detectable in fetal lung at 9-10 ... See full document
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On the development of the patella
... tendon development with that of patella formation by studying the expression of scleraxis (Scx), a tendon-specific marker (Schweitzer et ...Intriguingly, double fluorescence in situ ... See full document
9
A new method for identification of Trichomonas vaginalis by fluorescent DNA in situ hybridization
... vaginalis by DNA in situ hybridization, using a specific 2.3-kb fluorescent labeled DNA probe pROS21 and a subclone derived from this plasmid.. The development of improved in situ hybrid[r] ... See full document
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Chromogenic in situ hybridization to detect EGFR gene copy number in cell blocks from fine-needle aspirates of non small cell lung carcinomas and lung metastases from colo-rectal cancer
... ready-to-use double-stranded DNA digoxygenin-labelled EGFR probe (Zymed) or the biotin labelled chromosome 7 centromeric probe (Zymed) were ...and hybridization was performed by placing the slides in a ... See full document
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Molecular Epidemiology of Oral Treponemes Associated with Periodontal Disease
... whole-cell hybridization, a 10-ml aliquot of the hybridization mix contain- ing 20 mM Tris HCl, ...of hybridization at 46°C in a moist chamber in the dark, each of the slides was washed for 30 min in ... See full document
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