[PDF] Top 20 Development of a fluorescent probe based recombinase polymerase amplification assay for rapid detection of Orf virus
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Development of a fluorescent probe based recombinase polymerase amplification assay for rapid detection of Orf virus
... the virus in samples and also that A549 cell line could be a proper cell line for ORFV replication and ...No amplification was observed in non- ORFV infected cells/homogenizing tissue samples, this ... See full document
7
Development of real time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus
... Goatpox virus (GTPV) /AV40, GTPV/AV41, GTPV/GS- V1, sheeppox virus (SPPV) /Gulang2009, SPPV/Jing- tai2011, SPPV/Hubei, Orf virus (ORFV) /Vaccine/CHA, ORFV/HB/CHA, peste des petits ruminants ... See full document
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Development of real time and lateral flow strip reverse transcription recombinase polymerase Amplification assays for rapid detection of peste des petits ruminants virus
... [23], Orf virus (ORFV) [24], Schmallenberg virus (SBV) and bovine viral diarrhea virus (BVDV) ...RPA assay was characterized with high sensitivity, good diagnostic specificity and ... See full document
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Development of an isothermoal amplification based assay for rapid visual detection of an Orf virus
... this assay, just two target specific oligo- nucleotide primers are used to bind the template DNA with the assistance of a recombinase in combination with strand-displacement DNA ...an amplification ... See full document
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Field evaluation of a recombinase polymerase amplification assay for the diagnosis of Schistosoma japonicum infection in Hunan province of China
... of detection reached ...as detection targets, which were highly conserved and repeatable in the ...the fluorescent probe achieved detection within 10–15 min, which was a much shorter ... See full document
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Field Applicable Recombinase Polymerase Amplification Assay for Rapid Detection of Mycoplasma capricolum subsp capripneumoniae
... (LAMP) assay for the detection of ...This assay tran- scends the limitations associated with thermocyclers; however, it still requires DNA extraction protocols and gel electrophoresis to separate DNA ... See full document
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Evaluation of recombinase polymerase amplification for detection of begomoviruses by plant diagnostic clinics
... specific detection of plant pathogens is an important aspect of disease ...acid based methods for the de- tection of plant pathogens due to its speed, specificity and sensitivity ...pathogen ... See full document
9
Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis
... similar detection limits in comparison to gel electrophoresis analysis ...of probe during the LF-RPA reaction provides an add- itional starting point for polymerase elongation by functioning as ... See full document
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A novel recombinase polymerase amplification (RPA) assay for the rapid isothermal detection of Neospora caninum in aborted bovine fetuses
... The development of a method to rapidly diagnose Neospora caninum infection is highly ...desirable. Recombinase polymerase amplification (RPA), combined with lateral flow (LF) strips, is a ... See full document
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Development of a recombinase polymerase amplification (RPA) fluorescence assay for the detection of Schistosoma haematobium
... RPA assay was successfully developed to amplify the Dra1 DNA repeat region of ...of detection was 1 fg of ...diagnostic assay, that has a detection limit of 10 fg of ...the detection ... See full document
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Development of a Panel of Recombinase Polymerase Amplification Assays for Detection of Biothreat Agents
... isothermal recombinase polymerase amplification (RPA) assays, we developed a panel of 10 RPAs for biothreat ...variola virus, and reverse transcriptase RPA (RT-RPA) assays for Rift Valley ... See full document
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Rapid Detection of HIV 1 Proviral DNA for Early Infant Diagnosis Using Recombinase Polymerase Amplification
... RPA assay that has a very low limit of detection and rapid time to result (⬍20 min) and that can detect diverse virus variants—an important requirement for an effective HIV-1 ...the ... See full document
8
Development and evaluation of recombinase aided amplification assays incorporating competitive internal controls for detection of human adenovirus serotypes 3 and 7
... Traditional detection methods for HAdV 3 and HAdV 7, such as immunoassays and viral culture [12], are not suitable for rapid disease diagnosis because they are time-consuming and ...switched ... See full document
9
Early Detection of Dengue Virus by Use of Reverse Transcription Recombinase Polymerase Amplification
... the rapid diagnosis of early dengue virus (DENV) infection is highly ...tion-recombinase polymerase amplification (RT-RPA) assay was ...The assay detected DENV RNA in ... See full document
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Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of BK Virus
... is based on autocycling strand displace- ment DNA synthesis performed by the Bst DNA polymerase enzyme and was first described by Notomi et ...LAMP assay requires isothermal strand displace- ment and ... See full document
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Recombinase Polymerase Amplification Assay for Rapid Detection of Francisella tularensis
... Most fluorescent formats for isothermal amplification use nonspecific intercalating fluorophores or fluorescent primers (LAMP, SDA, HDA, and ...specific detection, probe for- mats have ... See full document
5
Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of Capripoxviruses
... poly(A) polymerase small-subunit gene (ORF068) and the intracellular mature virion envelope protein P32 gene (ORF074) (3, 5) of the fully sequenced LSDV-Neethling 2490 genome (GenBank accession ...were ... See full document
9
Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids
... microbial detection methods are based on PCR and real-time ...of-use detection and have led to the development of al- ternative amplification methods [44, ...thermal ... See full document
8
Development of a loop-mediated isothermal amplification assay for rapid detection of Yersinia enterocolitica via targeting a conserved locus
... Bst polymerase to improve it. In this way, before add- ing Bst polymerase to LAMP mixture, a pre-heating temperature (80°C) was carried out and immediately cooled down over an ice ...LAMP assay ... See full document
6
Loop Mediated Isothermal Amplification for Rapid Detection of Newcastle Disease Virus
... The development of both LAMP and nested PCR assays requires knowledge of the gene sequences, careful primer de- sign, and assay ...high amplification efficiency of LAMP. How- ever, the product ... See full document
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