[PDF] Top 20 Development of an isothermoal amplification based assay for rapid visual detection of an Orf virus
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Development of an isothermoal amplification based assay for rapid visual detection of an Orf virus
... Fig. 1 Schematic representation of ORFV RPA-LFD assay principle for the detection of ORFV. a Amplifying FAM-biotin-linking ORFV nfo RPA amplicons, in the presence of target template (nt position 4465 bp – ... See full document
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Development of real time and lateral flow dipstick recombinase polymerase amplification assays for rapid detection of goatpox virus and sheeppox virus
... Goatpox virus (GTPV) /AV40, GTPV/AV41, GTPV/GS- V1, sheeppox virus (SPPV) /Gulang2009, SPPV/Jing- tai2011, SPPV/Hubei, Orf virus (ORFV) /Vaccine/CHA, ORFV/HB/CHA, peste des petits ruminants ... See full document
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Development of a loop mediated isothermal amplification technique and comparison with quantitative real time PCR for the rapid visual detection of canine neosporosis
... caninum based on the biology of ...early detection of ...if detection of N. caninum DNA in blood corresponds to the detection of DNA in faeces remains ... See full document
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Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of Capripoxviruses
... via amplification of genetic material (DNA or RNA) by PCR has become a “gold standard” for rapid diagnosis of viral diseases, including ...for rapid diagnosis of CaPVs, including conven- tional and ... See full document
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Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of Subgroup J Avian Leukosis Virus
... subgroups based primarily on virus neutral- ization, their host range, and viral interference patterns (4, 5, ...leukosis virus (ALV-J), HPRS-103, was first isolated from commercial meat-type ... See full document
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Loop Mediated Isothermal Amplification (LAMP) Assay for GMO on: Recent Progresses and Future Perspectives
... purposes, development of rapid, economic and effective on-site detection methods for GM components is ...GMO detection are based on polymerase chain reaction ...on-site ... See full document
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Rapid and Real Time Detection of Chikungunya Virus by Reverse Transcription Loop Mediated Isothermal Amplification Assay
... RT-PCR assay for the detection of CHIK virus in acute-phase serum samples ...PCR assay has many advantages over conventional RT-PCR meth- ods, including rapidity, quantitative measurement, a ... See full document
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Development of real time and lateral flow strip reverse transcription recombinase polymerase Amplification assays for rapid detection of peste des petits ruminants virus
... and Orf, which also affect small ...for detection of viral nucleo- protein was developed as an alternative method to virus isolation ...immunochromatographic assay was developed for ... See full document
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Development and Evaluation of Reverse Transcription Loop Mediated Isothermal Amplification Assay for Rapid and Real Time Detection of Japanese Encephalitis Virus
... isothermal amplification (LAMP) is a novel nucleic acid amplification method developed by Eiken Chem- ical ...is based on the principle of strand displace- ment reaction and the stem-loop structure ... See full document
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Development and Evaluation of a Reverse Transcription Loop Mediated Isothermal Amplification Assay for Rapid Detection of Enterovirus 71
... includes virus iso- lation, detection of virus-specific antibodies, and detection of viral genomic sequences by nucleic acid amplification tech- ...assays based on enzyme ... See full document
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Development of a Loop Mediated Isothermal Amplification Assay for Rapid Detection of BK Virus
... LAMP assay is more complex to design than PCR ...DNA amplification as- says. Our LAMP assay performed successfully without DNA extraction for both urine and plasma ...the assay could still be ... See full document
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Development of a fluorescent probe based recombinase polymerase amplification assay for rapid detection of Orf virus
... RPA assay on clinical samples The practicality and efficiency of the ORFV exo RPA assay was evaluated with clinical samples and then compared with real-time ORFV qPCR ...of Orf, eight nasal swabs ... See full document
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Rapid and simultaneous detection of human hepatitis B virus and hepatitis C virus antibodies based on a protein chip assay using nano gold immunological amplification and silver staining method
... parallel detection of thousands of addressable elements in a single ...sensitive visual detection based on two-probe sandwich hybridization/nanoparticle amplifi- cation have been employed, and ... See full document
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Development of a real time reverse transcription loop mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virus
... for rapid detection of ...LAMP assay allow for the most convenient identification of PEDV among the three ...effective, rapid and economical early diagnostic technique for the detection ... See full document
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A loop mediated isothermal amplification assay for the visual detection of duck circovirus
... the amplification in order to elimin- ate the chance of ...to amplification, calcein combines with the manganese ions (Mn 2+ ) and the reaction solution turns ...LAMP amplification reaction proceeds, ... See full document
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Evaluation of FLASH - PCR forrapid detection of Mycobacterium tuberculosis from clinical specimens.
... as rapid diagnosis can be used for HIV - infected patients because of a higher susceptibility to MTB infection and so can be a great help for controlling ... See full document
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Laboratory Diagnosis of Mycobacterium tuberculosis Infection and Disease in Children
... The process of digestion, decontamination, and concentration of pediatric specimens prior to culture set up is typically per- formed as that for specimens from adults (10). Due to the pauc- ibacillary nature of specimens ... See full document
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Loop mediated isothermal amplification assay for rapid detection of staphylococcus aureus
... LAMP assay whereas PCR thermocycler, gel elec- trophoresis, and gel documentation systems are needed for PCR ...LAMP assay holds an advantage as the positive result of the LAMP assay could be viewed ... See full document
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Recombinase polymerase amplification assay combined with a lateral flow dipstick for rapid detection of Tetracapsuloides bryosalmonae, the causative agent of proliferative kidney disease in salmonids
... polymerase amplification (RPA) is a novel isothermal nucleic acid amplification technology that does not require any thermal cycling, as the amplifi- cation reaction is performed at a constant low ... See full document
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The development of a GeXP based multiplex reverse transcription PCR assay for simultaneous detection of sixteen human respiratory virus types/subtypes
... PCR, amplification was carried out predominantly by a single pair of universal primers, of which only the for- ward universal primer was labeled ...the assay were function- ing ...electrophoresis ... See full document
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