[PDF] Top 20 The Development of a Loop Mediated Isothermal Amplification (LAMP) Procedure for Plague Diagnostic
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The Development of a Loop Mediated Isothermal Amplification (LAMP) Procedure for Plague Diagnostic
... research, plague is still not ...considers plague a reemerging disease with a trend of an increasing number of cases with geographical dispersion ...focal plague areas is mandatory in order to ... See full document
10
Loop Mediated Isothermal Amplification for Rapid Detection of Newcastle Disease Virus
... The development of both LAMP and nested PCR assays requires knowledge of the gene sequences, careful primer de- sign, and assay ...Designing LAMP primers is cru- cial in optimizing the LAMP ... See full document
5
Liu, Zhijie (2010): Identification of Novel Genes for the Development of a Rapid Diagnostic Test for Theileria uilenbergi Infection by Screening of a Merozoite cDNA Library. Dissertation, LMU München: Tierärztliche Fakultät
... technique, loop-mediated isothermal amplification (LAMP), was developed (Notomi et ...gene amplification which relies on the auto-cycling strand displacement synthesis of DNA by ... See full document
81
Application of loop mediated isothermal amplification for malaria diagnosis during a follow up study in São Tomé
... hours. LAMP is simpler and faster and can be a potential tool to replace nested PCR, but it still takes time to obtain results as the laboratory is usually distant from collection ...the LAMP assay are only ... See full document
9
Development and evaluation of a Loop Mediated Isothermal Amplification (LAMP) technique for the detection of hookworm (Necator americanus) infection in fecal samples
... the LAMP assay, a simple detection sys- tem based on staining the amplification products with SYBR green dye, which can be visualized with the naked eye, demonstrating the feasibility of using this assay ... See full document
7
Development of loop mediated isothermal amplification (LAMP) for simple detection of Leishmania infection
... Also, LAMP is particularly prone to contamination due to the large amount of DNA that it can generate, and its capability to amplify minute amounts of DNA. During this study, we nevertheless encountered multiple ... See full document
8
Comparison of loop mediated isothermal amplification assay and smear microscopy with culture for the diagnostic accuracy of tuberculosis
... of-care diagnostic tests especially in resource limited settings are jeopardized the control of the TB ...stay diagnostic method available for the detection of TB at peripheral laboratories in developing ... See full document
6
Wolbachia detection in insects through LAMP: loop mediated isothermal amplification
... pathogen development in many different mosquito- pathogen combinations [2-5], and recently being applied in the field in Australia, Vietnam, Indonesia and soon in other dengue endemic countries ... See full document
5
Development and evaluation of an in house single step loop mediated isothermal amplification (SS LAMP) assay for the detection of Mycobacterium tuberculosis complex in sputum samples from Moroccan patients
... In conclusion, the SS-LAMP developed in this study pro- vides a number of advantages over microscopy and culture including superior sensitivity and significantly less operating time. Furthermore, the ... See full document
10
Real Time Reverse Transcription Loop Mediated Isothermal Amplification for Rapid Detection of Rift Valley Fever Virus
... the loop primers loop F and loop B, ...the amplification curves were not sigmoidal, contrary to classical real-time PCR or even LAMP when a turbidimeter (650 nm) is used to detect ... See full document
7
A novel loop mediated isothermal amplification based test for detecting Neospora caninum DNA
... The development of DNA-based methods such as conventional PCR [39, 40], nested and semi-nested PCR [41, 42] and different quantitative PCR types [43, 44] have contributed towards diagnosing ...the LAMP ... See full document
9
Combination of PURE DNA extraction and LAMP DNA amplification methods for accurate malaria diagnosis on dried blood spots
... and LAMP‑DNA amplification methods for accurate malaria diagnosis on dried blood ...PURE‑LAMP: Procedure for ultra rapid extraction–loop‑mediated isothermal ... See full document
7
Rapid identification of Aconitum plants based on loop mediated isothermal amplification assay
... the LAMP assay on a real-time PCR device to monitor the amplification ...the LAMP assay can be performed on a block incubator, and ampli- fication can be checked by visual inspection (Additional file ... See full document
5
Loop Mediated Isothermal Amplification Method for Rapid Detection of the Periodontopathic Bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola
... acid amplification is one of the most valuable meth- ods for research in the life sciences; the new technique has particularly benefited amplicon-oriented sciences, including studies concerned with the diagnosis ... See full document
7
Diagnostic performance of a novel loop mediated isothermal amplification (LAMP) assay targeting the apicoplast genome for malaria diagnosis in a field setting in sub Saharan Africa
... 95 % power, 5 % precision, an effect size of 0.25, and 5 degree of freedom (df). The performance characteristics of the LAMP assay as well as that of microscopy and RDT were calculated using the standard PCR assay ... See full document
6
Field evaluation of malaria malachite green loop mediated isothermal amplification in health posts in Roraima state, Brazil
... malaria diagnostic test in remote health posts in a malaria-endemic ...published LAMP- based assays, which claim detection limits below 16 para- ...of diagnostic accuracy as the PET-PCR assay in ... See full document
7
Future developments in biosensors for field-ready Zika virus diagnostics
... Helicase-dependent amplification; LAMP: Loop-mediated isothermal amplification; MAC-ELISA: IgM antibody capture enzyme-linked immunosorbent assay; NAATs: Nucleic acid ... See full document
9
Loop Mediated Isothermal Amplification for Rapid and Reliable Diagnosis of Tuberculous Meningitis
... the LAMP assay was more sensitive than nested ...be LAMP positive but nested-PCR ...these LAMP-positive but nested-PCR-negative samples have a low OD value with LAMP strongly suggests the ... See full document
5
An innovative diagnostic technology for the codon mutation C580Y in kelch13 of Plasmodium falciparum with MinION nanopore sequencer
... [10]. Amplification is mediated by a loop struc- ture and relies on an auto-cycling procedure performed with strand-displacement of Bst DNA polymerase under isothermal ...the ... See full document
11
Original Article Development and validation of a rapid and simple fluorescence real-time loop-mediated isothermal amplification assay for detection of Burkholderia pseudomallei
... The LAMP method requires much less time than traditional culture and is more cost-effec- tive [29, ...the LAMP assay was shown to be completed in a short time and that the reaction products could be ... See full document
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