[PDF] Top 20 Development of a Quantitative Real Time PCR Assay for Detection of Mycoplasma genitalium
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Development of a Quantitative Real Time PCR Assay for Detection of Mycoplasma genitalium
... LightCycler assay failed to detect four specimens ...TaqMan assay, and the copy number proved to be very low ( ⬍ 3 copies/ ...TaqMan assay has the advan- tage of being performed with a total volume ... See full document
8
Development and Validation of a Quantitative Real Time PCR Assay Using Fluorescence Resonance Energy Transfer Technology for Detection of Aspergillus fumigatus in Experimental Invasive Pulmonary Aspergillosis
... The detection of galactomannan antigen in serum by enzyme immunoassay is an important advance in the detection of invasive aspergillosis (13, 21, ... See full document
7
Quantitative Detection of Mycoplasma genitalium from First Pass Urine of Men with Urethritis and Asymptomatic Men by Real Time PCR
... M. genitalium has been reported to be low and significantly less than that of ...M. genitalium loads of ⬍5 ⫻ 10 ...M. genitalium, which requires strict conditions for growth, to colonize and ... See full document
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Detection of Mycoplasma genitalium by PCR Amplification of the 16S rRNA Gene
... different PCR assays on clinical specimens (5, ...diagnostic PCR assay, and therefore, the 16S rRNA gene was selected as the target for development of a new ...that time, the sequence ... See full document
6
A Novel SimpleProbe PCR Assay for Detection of Mutations in the 23S rRNA Gene Associated with Macrolide Resistance in Mycoplasma genitalium in Clinical Samples
... ycoplasma genitalium is a small bacterium with the ability to cause urethritis in men and urethritis, cervicitis, and pelvic inflammatory disease (PID) in women ...M. genitalium in Scandi- navia is ...avoid ... See full document
5
Performance of the Gen Probe Transmission Mediated Amplification Research Assay Compared to That of a Multitarget Real Time PCR for Mycoplasma genitalium Detection
... MTRT PCR primers and probes. Previously published sets of primers and probes were utilized in the further development of this assay (14, 29). For the MgPa gene target, the primers and probe were ... See full document
6
Multicenter Clinical Evaluation of a Novel Multiplex Real Time PCR (qPCR) Assay for Detection of Fluoroquinolone Resistance in Mycoplasma genitalium
... qPCR assay is more efficient and potentially less expensive than performing Sanger sequencing, although, unlike Sanger sequencing, it has a limitation in that it detects only a defined set of ...(beta) assay ... See full document
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Development of a real time quantitative PCR assay for detection of a stable genomic region of BK virus
... develop supporting evidence for the sequence stability of these regions, an interspecies amino acid sequence com- parison of each of the regions was performed. Among the four potential target regions, the VP2 C-terminus ... See full document
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Duplex quantitative real time PCR assay for the detection and discrimination of the eggs of Toxocara canis and Toxocara cati (Nematoda, Ascaridoidea) in soil and fecal samples
... Microscopic examination of dog and cat stools or soil samples is commonly used for identification of Toxocara eggs. The method includes an enrichment pre-analytical step through the use of centrifuge-flotation techniques ... See full document
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Use of TaqMan 5′ Nuclease Real Time PCR for Quantitative Detection of Mycoplasma genitalium DNA in Males with and without Urethritis Who Were Attendees at a Sexually Transmitted Disease Clinic
... TaqMan assay was less prone to inhibition than our conventional PCR for detection of the 16S rRNA gene, as experienced during parallel testing by the two ...M. genitalium-negative specimens, ... See full document
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Comparative evaluation of a laboratory developed real time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
... qPCR assay was shown to be 100% specific for HAdV with no cross-reactivity against 32 human viral patho- ...virus PCR Kit which is also 100% specific when tested against 35 different viral and bacterial ... See full document
9
Comparison of Transcription Mediated Amplification and PCR Assay Results for Various Genital Specimen Types for Detection of Mycoplasma genitalium
... differential detection of M. genitalium by specimen type may reflect differences in the bacterial load between vaginal swab, cervical swab, and urine ...M. genitalium at different genital sites in ... See full document
7
Development of a loop mediated isothermal amplification technique and comparison with quantitative real time PCR for the rapid visual detection of canine neosporosis
... by PCR, a cat that was positive in blood would likely have shed oocysts within the previous 35 days [54, ...early detection of ...if detection of N. caninum DNA in blood corresponds to the ... See full document
10
Development and Evaluation of a Novel Single Nucleotide Polymorphism Real Time PCR Assay for Rapid Detection of Fluoroquinolone Resistant Mycoplasma bovis
... PG49, Mycoplasma alkalescens PG31/D12, Mycoplasma alvi Isley, Mycoplasma arginini G230, Mycoplasma bovigenitalium PG11, Mycoplasma bovirhinis PG43, Mycoplasma bovoculi M165/69, ... See full document
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Development and validation of a real time PCR assay for the detection of clinical acanthamoebae
... is time consuming, particu- larly when we need to arbitrate discordance with use of a third end-point PCR-based assay [5, ...Riviere assay [6] was designed as a molecular diagnostic test ... See full document
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Comparison of a New Quantitative ompA Based Real Time PCR TaqMan Assay for Detection of Chlamydia pneumoniae DNA in Respiratory Specimens with Four Conventional PCR Assays
... one quantitative run. Amplifi- cation and detection of the PCR product were performed with an ABI Prism 7700 sequence detection instrument (Applied Biosystems), as suggested by the ... See full document
9
A duplex real time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae
... pathogen detection to be used as a clinical diagnostic tool, sensitivity is a key criterion ...developed assay, as few as 2 x 10 1 copies could be detected for both ARV and MS, which was more sensitive than ... See full document
9
Molecular Detection and Species Specific Identification of Medically Important Aspergillus Species by Real Time PCR in Experimental Invasive Pulmonary Aspergillosis
... sensitive quantitative PCR (qPCR) assays for genus- and species-specific identification of Aspergillus infections by use of TaqMan ...of real-time qPCR primers and probes was developed by ... See full document
8
Development and evaluation of a real time PCR assay for the quantitative detection of Theileria annulata in cattle
... Although haematological parameters of these animals remained close and occasionally below the lowest values considered normal for cattle, no correlation was found between parasitaemia and haematology values. Major ... See full document
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A triplex quantitative real time PCR assay for differential detection of human adenovirus serotypes 2, 3 and 7
... traditional PCR method to detect serotypes of adenovirus [25, 26] needs gel electrophor- esis and sequencing, which significantly increases the risk of cross-contamination and is also time consuming and ... See full document
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