[PDF] Top 20 Development of a Real Time PCR Assay for Detecting and Quantifying Human Bocavirus 2
Has 10000 "Development of a Real Time PCR Assay for Detecting and Quantifying Human Bocavirus 2" found on our website. Below are the top 20 most common "Development of a Real Time PCR Assay for Detecting and Quantifying Human Bocavirus 2".
Development of a Real Time PCR Assay for Detecting and Quantifying Human Bocavirus 2
... Plasmid standard construction. A 587-bp DNA subgenomic fragment of HBoV2 was amplified from a positive stool specimen using primers bracketing the real-time PCR target region in the NP-1 gene ... See full document
5
Development of a Real Time Quantitative PCR for Detecting Duck Hepatitis A Virus Genotype C
... the real-time PCR ...a real-time PCR assay for detecting mRNA expression of GAPDH were as follows: the forward primer (GAPDH FP) (5=-CACAGCCACACACGAAGACA-3=) and ... See full document
6
Development and validation of a real time PCR assay for the detection of clinical acanthamoebae
... is time consuming, particu- larly when we need to arbitrate discordance with use of a third end-point PCR-based assay [5, ...Riviere assay [6] was designed as a molecular diagnostic test ... See full document
8
Development of multiplexed real-time quantitative polymerase chain reaction assay for detecting human adenoviruses.
... the assay illustrated the complexities in multiplexing real time PCR ...or human genome tested. Using the 2-mixture AdV assay we have successfully detected AdV in serial ... See full document
28
Human bocavirus (HBoV) in children with respiratory tract infection by enzyme linked immunosorbent assay (ELISA) and qualitative polymerase chain reaction (PCR)
... 0.001). PCR detected four HBoV positive cases that were negative by ELISA, this could be explained by the high sensitivity of PCR over ELISA since it was (100% vs ...the real-time HBoV ... See full document
5
Laboratory Detection of Clostridium difficile in Piglets in Australia
... for human infections, and there are no published studies assessing their performance in Australian ...a real-time PCR for tcdB (GeneOhm Cdiff; Bec- ton Dickinson), two-component enzyme ... See full document
7
Development and Evaluation of an Enterovirus D68 Real Time Reverse Transcriptase PCR Assay
... a real-time reverse transcriptase PCR (RT-PCR) assay for the detection of human enterovirus D68 (EV-D68) in clinical ...This assay was developed in response to the ... See full document
7
Multiplex, Quantitative, Real Time PCR Assay for Cytomegalovirus and Human DNA
... The development of real-time PCR technology has simpli- fied nucleic acid quantification and is coming into more wide- spread ...use. Real-time, quantitative PCR has ... See full document
6
Development and Assessment of a Multiplex Real Time PCR Assay for Quantification of Human Immunodeficiency Virus Type 1 DNA
... of human immunodeficiency virus type 1 (HIV-1) disease progression varies greatly among HIV-1-infected indi- viduals (29), depending on several factors, such as baseline HIV RNA level, baseline CD4 ⫹ T-cell level, ... See full document
6
Development of a Quantitative Real Time PCR Assay for Detection of Mycoplasma genitalium
... Culture of other organisms. The following microorganisms and human cells were used in the study. Mycoplasma fermentans PG18 was grown in BEg medium (8). Mycoplasma arthritidis PG6, Mycoplasma buccale 20247, ... See full document
8
Real Time Reverse Transcription PCR Assay for Comprehensive Detection of Human Rhinoviruses
... HEV real-time RT-PCR assays (25, 38, 47), the development of comparable assays for HRVs has been hampered by their greater genetic variability and the paucity of published HRV sequence data ... See full document
7
Development of one step SYBR Green real time RT PCR for quantifying bovine viral diarrhea virus type 1 and its comparison with conventional RT PCR
... For accurate quantification of PCR products, the slope of the standard curve obtained with 10-fold dilutions should approach -3.3 in theory, but a slope from -3.1 to -3.6 was acceptable in practice. Furthermore, ... See full document
8
Real Time PCR Assays for Detection of Bocavirus in Human Specimens
... Clinical specimens. Respiratory specimens were collected from 1,178 hospi- talized pneumonia patients identified through active population-based surveil- lance for pneumonia in Sa Kaeo Province, Thailand, between 1 ... See full document
5
Real Time PCR for Diagnosis of Human Bocavirus Infections and Phylogenetic Analysis
... Quantitative real-time PCR of ...hBoV real-time PCR, 11 samples from the period of 1998 to 2001 and 823 samples from 2002 to September 2005 NPA that had previously been tested by ... See full document
7
Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... some human patients with severe pneumonia and even causes ...of human infection with H5N6 virus, including six deaths, have been reported to WHO from China since 2014 ...healthy development of the ... See full document
7
Multiplex Real Time PCR Assay for Detection of Influenza and Human Respiratory Syncytial Viruses
... multiplex real-time PCR assay was developed with a LightCycler instrument for detection of influenza viruses A and B and the human respiratory syncytial virus ...multiplex PCR ... See full document
7
Comparison of Multiplex PCR-ELISA and conventional Multiplex PCR for detection of HIV-1/HCV co-infection
... increase in sensitivity and specificity (because of using specific probes) are advantages of this method. We did not observe non-specific interactions between primers and the human genome, HBV or other pathogens. ... See full document
6
Evaluation of Novel Broad Range Real Time PCR Assay for Rapid Detection of Human Pathogenic Fungi in Various Clinical Specimens
... of real-time PCR and direct sequencing of positive PCR products improves the di- agnostic turnaround ...total time required for the de- tection of fungal pathogens in cervical swabs and ... See full document
8
Comparative evaluation of a laboratory developed real time PCR assay and RealStar® Adenovirus PCR Kit for quantitative detection of human adenovirus
... the development of HAdV quantitative PCR (qPCR) protocols ...qPCR assay that can detect HAdV serotypes 11, 34, and 35 and shown that per- sistence of HAdV in the lower respiratory tract is com- mon ... See full document
9
Multicenter Performance Evaluation of a New TaqMan PCR Assay for Monitoring Human Immunodeficiency Virus RNA Load
... The HPS-CTMHIV assay’s precision, as assessed by intra- and interassay reproducibility, is good and is comparable to that of the CAHIM assay. Further improvement is expected when the RNA extraction procedure, ... See full document
5
Related subjects