[PDF] Top 20 Development of simple multiplex real-time PCR assays for foodborne pathogens detection and identification on LightCycler
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Development of simple multiplex real-time PCR assays for foodborne pathogens detection and identification on LightCycler
... Plus (Capillary) with 7,5 mM MgCl 2 , 5 µl of primer cocktail (0.5 µl of each forward and reverse primer) and 10 ml of molecular grade water. Tenfold dilutions of the target genomic DNA (1 ng/ml and 100 pg/ml) were ... See full document
6
Multiplex Real-Time PCR Assay for Detection of Respiratory Pathogens among Pneumonia Affected Children
... countries, Multiplex real-time PCR pathogen detection assays are currently being used for the detection of the presence of potentially harmful pathogens in high ... See full document
5
Development and Validation of Multiplex One Step Real Time TaqManqRT PCR Assays for Detection and Quantification of Arboviral Encephalitis Viruses
... one-step real-time qRT-PCR assays for multiplex detection of 15 viruses, which covered nearly all the important viral pathogens that cause arboviral encephalitis, in- ... See full document
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Development of multiplex real time PCR assays for identification of members of the Anopheles funestus species group
... the PCR products and a resulting decrease in SYBR green fluorescence as the dye is ...the detection of ...species identification trial (Table 2) where the MCA exhibited a low failure rate but ... See full document
9
Evaluation of Multiplex Tandem Real Time PCR for Detection of Cryptosporidium spp , Dientamoeba fragilis, Entamoeba histolytica, and Giardia intestinalis in Clinical Stool Samples
... first development and evaluation of a multiplex tandem PCR (MT-PCR) assay for the detection and identification of 4 common pathogenic protozoan parasites, Cryptospo- ridium ... See full document
6
Molecular approaches for detection and identification of foodborne pathogens
... the foodborne viruses is very ...immune-sorbent assays (ELISA); but their sensitivity is not high enough to detect low number of ...viruses’ detection (Bosch et ...by real-time-RT ... See full document
6
Rapid Identification of Mycobacterium tuberculosis and Nontuberculous Mycobacteria by Multiplex, Real Time PCR
... for identification. To address the need for a simple and rapid molecular assay with a broader identification scope, we developed a multiplex, real-time PCR assay that can ... See full document
6
Development of multiplex real-time PCR assays for identification of members of the Anopheles funestus species group
... the PCR products and a resulting decrease in SYBR green fluorescence as the dye is ...the detection of ...species identification trial (Table 2) where the MCA exhibited a low failure rate but ... See full document
10
Duplex Real Time SYBR Green PCR Assays for Detection of 17 Species of Food or Waterborne Pathogens in Stools
... and identification of food- or waterborne pathogens from stool specimens in food poisoning outbreaks are time-consuming and ...the time required for identification of food- or ... See full document
13
Evaluation of Multiplex Type Specific Real Time PCR Assays Using the LightCycler and Joint Biological Agent Identification and Diagnostic System Platforms for Detection and Quantitation of Adult Human Respiratory Adenoviruses
... The impact and distribution of different types varies over time and can be drastically altered through use of type-specific vaccines (14, 22, 23). An effective vaccine for HAdV-B7 and HAdV-E4 was used in the ... See full document
7
Clinical Validation of Multiplex Real Time PCR Assays for Detection of Bacterial Meningitis Pathogens
... Real-time PCR is a powerful tool for detection of human patho- gens because of the high sensitivity and throughput ...enables development of a multiplex platform for amplifica- ... See full document
7
Development of a melting curve based multiplex real time PCR assay for simultaneous detection of Streptococcus agalactiae and genes encoding resistance to macrolides and lincosamides
... direct detection of resistance genes in GBS from pregnant women will contribute to prompt and ap- propriate administration of antimicrobial during the intrapartum ...GBS detection, the determination of its ... See full document
11
Detection of Mycobacterium bovis in Bovine Clinical Specimens Using Real Time Fluorescence and Fluorescence Resonance Energy Transfer Probe Rapid Cycle PCR
... Lymph nodes were collected from 38 cattle exhibiting lesions at slaughter. These cattle were either intradermal skin test positive or identified as having lesions during routine meat inspection. Specimens were subjected ... See full document
7
Comparison of Real Time Multiplex Human Papillomavirus (HPV) PCR Assays with the Linear Array HPV Genotyping PCR Assay and Influence of DNA Extraction Method on HPV Detection
... HPV PCR versus m-LA with Qiagen Spin blood kit DNA ...the multiplex HPV PCR assay and the Linear Array (LA) assay, (4 l DNA sample per PCR versus 50 l DNA sample, respectively), we ... See full document
8
<p>Detection of <em>Mycoplasma pneumoniae</em> in Mexican children with community-acquired pneumonia: experience in a tertiary care hospital</p>
... the real-time PCR, primers were designed using the Primer 3 program ...of detection of the method. The detection limit was C T ≤ ... See full document
11
Multiplex Real Time PCR for Detection of Campylobacter, Salmonella, and Shigella
... FIG 1 (A) Real-time PCR simultaneously detects three different genes. Data from individual tubes, each containing the ATCC strains S. enteritidis 13076,S. flexneri 12022, and C. jejuni subsp. jejuni ... See full document
8
Real Time PCR Assays for Detection of Bocavirus in Human Specimens
... specific real-time TaqMan PCR assays that target the HBoV NS1 and NP-1 ...Both assays could reproducibly detect 10 copies of a recombinant DNA plasmid containing a partial region of the ... See full document
5
Development and Evaluation of Real Time PCR Based Fluorescence Assays for Detection of Chlamydia pneumoniae
... by PCR and culturing, two of four culture-positive specimens were found positive by the VD2 and VD4 real-time PCR ...and PCR have been reported. In a study to assess the utility of ... See full document
9
Development of Conventional and Real Time PCR Assays for Detection of Legionella DNA in Respiratory Specimens
... evaluated assays to detect Legionella DNA in respiratory tract specimens and sometimes also in serum or urine (1, 6–8, 9–12, 14–16, ...23). PCR is more time-consuming and complex to perform than ... See full document
7
Multiplex Real Time PCR Targeting the RNase P RNA Gene for Detection and Identification of Candida Species in Blood
... the multiplex PCR, 10,000 cells of the four species diluted in 1 ml phosphate-buffered saline were centrifuged, the buffer was removed, and the DNA was extracted as described ...in multiplex and ... See full document
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