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[PDF] Top 20 PR Domain of Rous Sarcoma Virus Gag Causes an Assembly/Budding Defect in Insect Cells

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PR Domain of Rous Sarcoma Virus Gag Causes an Assembly/Budding Defect in Insect Cells

PR Domain of Rous Sarcoma Virus Gag Causes an Assembly/Budding Defect in Insect Cells

... RSV Gag in the baculovi- rus overexpression system, in effect preventing proper ...possible causes of the RSV-specific bud- ding defect in insect cells, we created three recombinant ... See full document

6

Specificity of Plasma Membrane Targeting by the Rous Sarcoma Virus Gag Protein

Specificity of Plasma Membrane Targeting by the Rous Sarcoma Virus Gag Protein

... the budding defect associated with the B1c deletion resulted from a disruption of membrane binding for ...QT6 cells using subcellular fractionation followed by membrane ...of Gag that deletes ... See full document

11

Insertion of a Classical Nuclear Import Signal into the Matrix Domain of the Rous Sarcoma Virus Gag Protein Interferes with Virus Replication

Insertion of a Classical Nuclear Import Signal into the Matrix Domain of the Rous Sarcoma Virus Gag Protein Interferes with Virus Replication

... The Rous sarcoma virus Gag protein undergoes transient nuclear trafficking during virus ...in Gag would affect virus assembly or ...MA domain of Gag ... See full document

9

Rous Sarcoma Virus Gag Protein-Oligonucleotide Interaction Suggests a Critical Role for Protein Dimer Formation in Assembly

Rous Sarcoma Virus Gag Protein-Oligonucleotide Interaction Suggests a Critical Role for Protein Dimer Formation in Assembly

... protein Gag is necessary and sufficient to direct the assembly, budding, and release of virus- like particles (VLPs) from the plasma ...including Gag-RNA inter- action, ... See full document

11

In vitro assembly of virus-like particles with Rous sarcoma virus Gag deletion mutants: identification of the p10 domain as a morphological determinant in the formation of spherical particles.

In vitro assembly of virus-like particles with Rous sarcoma virus Gag deletion mutants: identification of the p10 domain as a morphological determinant in the formation of spherical particles.

... protein, Gag, leads to budding of virus-like particles into the extracellular ...which virus-like particles are formed spontaneously in vitro from fragments of Rous sarcoma ... See full document

11

Repositioning Basic Residues in the M Domain of the Rous Sarcoma Virus Gag Protein

Repositioning Basic Residues in the M Domain of the Rous Sarcoma Virus Gag Protein

... M domain mutants in avian ...M domain prevent Gag targeting to the plasma membrane, we used a construct in which GFP is fused to RSV Gag ...living cells. Gag-GFP was cre- ated by ... See full document

8

Fine mapping and characterization of the Rous sarcoma virus Pr76gag late assembly domain.

Fine mapping and characterization of the Rous sarcoma virus Pr76gag late assembly domain.

... the Rous sarcoma virus (RSV) Gag polyprotein contains an assembly domain, which is required late in replication for efficient budding of virus-like particles from ... See full document

6

Analysis of cleavage site mutations between the NC and PR Gag domains of Rous sarcoma virus.

Analysis of cleavage site mutations between the NC and PR Gag domains of Rous sarcoma virus.

... of Gag, Gag-Pro, or Gag-Pro-Pol precursor ...avian sarcoma and leukemia viruses (ASLV), PR forms the C-terminal domain of ...NC domain and the PR domain, the ... See full document

7

Mutations in the Spacer Peptide and Adjoining Sequences in Rous Sarcoma Virus Gag Lead to Tubular Budding

Mutations in the Spacer Peptide and Adjoining Sequences in Rous Sarcoma Virus Gag Lead to Tubular Budding

... protein, Gag, which is necessary and sufficient for the assembly and budding of enveloped virus-like particles from the ...The Gag proteins of Rous sarcoma virus ... See full document

10

RNA Dimerization Defect in a Rous Sarcoma Virus Matrix Mutant

RNA Dimerization Defect in a Rous Sarcoma Virus Matrix Mutant

... retrovirus Gag polyprotein directs the assembly and budding of virus particles from the plasma membrane of in- fected ...several Gag pro- teins has led to the identification of three ... See full document

9

Transport and processing of the Rous sarcoma virus Gag protein in the endoplasmic reticulum.

Transport and processing of the Rous sarcoma virus Gag protein in the endoplasmic reticulum.

... RSV Gag proteins transiently expressed in COS-1 cells were labeled in the presence of increasing amounts of ...above, budding of the wild-type Gag protein was not affected at all by the ... See full document

10

Overlapping Roles of the Rous Sarcoma Virus Gag p10 Domain in Nuclear Export and Virion Core Morphology

Overlapping Roles of the Rous Sarcoma Virus Gag p10 Domain in Nuclear Export and Virion Core Morphology

... RSV Gag protein is an integral step in the virus assembly ...the Gag polyprotein through the nucleus is essential for the production of infectious virions is less ...the assembly of ... See full document

11

An LYPSL Late Domain in the Gag Protein Contributes to the Efficient Release and Replication of Rous Sarcoma Virus

An LYPSL Late Domain in the Gag Protein Contributes to the Efficient Release and Replication of Rous Sarcoma Virus

... DF1 cells were transfected with proviral RSV DNA containing either wild-type or mutant PPPY and LYPSL motifs and carrying a GFP ...the cells were diluted with a 10-fold excess of nontransfected cells ... See full document

12

Characterization of Rous Sarcoma Virus Gag Particles Assembled In Vitro

Characterization of Rous Sarcoma Virus Gag Particles Assembled In Vitro

... purified Gag protein or fragments of Gag, as studied most extensively for Mason-Pfizer monkey virus (50), RSV (14, 15, 46), and HIV-1 (13, 35, 41–44, 58, ...from Gag protein translated in ... See full document

12

Evidence for a second function of the MA sequence in the Rous sarcoma virus Gag protein.

Evidence for a second function of the MA sequence in the Rous sarcoma virus Gag protein.

... by budding from the plasma membrane of the host cell, and the Gag protein is the only viral gene product required for this ...ribosomes, Gag polypro- teins are transported to the plasma membrane, ... See full document

11

An assembly domain of the Rous sarcoma virus Gag protein required late in budding.

An assembly domain of the Rous sarcoma virus Gag protein required late in budding.

... In contrast, containing the NC-PR cleavage site are processed vitro 10, in agreement with the hypothesis among the first to be cleaved during particle maturation the p2-plO junction serv[r] ... See full document

14

Membrane Binding of the Rous Sarcoma Virus Gag Protein Is Cooperative and Dependent on the Spacer Peptide Assembly Domain

Membrane Binding of the Rous Sarcoma Virus Gag Protein Is Cooperative and Dependent on the Spacer Peptide Assembly Domain

... RSV Gag and murine leukemia virus (MLV) Gag, which on average adopts an elongated conformation (16, 43, ...binding domain (MBD)—the N-terminal end of MA—and p10 ...membrane-bound Gag ... See full document

13

Genetic analysis of the major homology region of the Rous sarcoma virus Gag protein.

Genetic analysis of the major homology region of the Rous sarcoma virus Gag protein.

... MHR domain of ...protease-defective Gag proteins: the D37I Gag protein (bearing a point mutation in the PR active site), mu- tant 3h (truncated after the first seven residues of PR), ... See full document

15

Detailed Mapping of the Nuclear Export Signal in the Rous Sarcoma Virus Gag Protein

Detailed Mapping of the Nuclear Export Signal in the Rous Sarcoma Virus Gag Protein

... anemia virus and feline immu- nodeficiency virus Rev-like proteins ...RSV Gag differs from the consensus sequence, the homolo- gous Gag protein of v-ErbA was reported to bind directly to Crm1 ... See full document

10

Comparison of Rous Sarcoma Virus-Specific Deoxyribonucleic Acid Polymerases in Virions of Rous Sarcoma Virus and in Rous Sarcoma Virus-Infected Chicken Cells

Comparison of Rous Sarcoma Virus-Specific Deoxyribonucleic Acid Polymerases in Virions of Rous Sarcoma Virus and in Rous Sarcoma Virus-Infected Chicken Cells

... The material which banded at 1.24 g/cc in These systems contained DNA polymerase acan equilibrium sucrose density gradient probably tivities and an endogenous RNA template and did not co[r] ... See full document

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