[PDF] Top 20 Encapsidation of the Flavivirus Kunjin Replicon RNA by Using a Complementation System Providing Kunjin Virus Structural Proteins in trans
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Encapsidation of the Flavivirus Kunjin Replicon RNA by Using a Complementation System Providing Kunjin Virus Structural Proteins in trans
... Kunjin virus (KUN) replicon RNA was encapsidated by a procedure involving two consecutive electropora- tions of BHK-21 cells, first with KUN replicon RNA C20DXrep (with prME and ... See full document
11
Subgenomic replicons of the flavivirus Kunjin: construction and applications.
... Several Kunjin virus (KUN) subgenomic replicons containing large deletions in the structural region (C-prM-E) and in the 3 * untranslated region (3 * UTR) of the genome have been ...constructed. ... See full document
9
Translation of the Flavivirus Kunjin NS3 Gene in cis but Not Its RNA Sequence or Secondary Structure Is Essential for Efficient RNA Packaging
... complemented replicon RNAs with double frameshift mu- tations in ...helper replicon (C20DXrepNeo) and the complemented puromycin-selectable KUN replicons with the deletions/frameshift mutations in NS3 and a ... See full document
10
Coupling between Replication and Packaging of Flavivirus RNA: Evidence Derived from the Use of DNA-Based Full-Length cDNA Clones of Kunjin Virus
... Experimental system. In order to provide structural proteins in cis for KUN RNA packaging, we prepared two plasmid DNA constructs, pKUN1 and pKUN1dGDD, which allowed production in transfected ... See full document
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Complementation Analysis of the Flavivirus Kunjin NS3 and NS5 Proteins Defines the Minimal Regions Essential for Formation of a Replication Complex and Shows a Requirement of NS3 in cis for Virus Assembly
... Dual replicon complementation system. The dual replicon complementation system employs the use of repBHK cells persistently producing KUN replicon RNA which also ... See full document
10
Efficient trans-Complementation of the Flavivirus Kunjin NS5 Protein but Not of the NS1 Protein Requires Its Coexpression with Other Components of the Viral Replicase
... efficient trans-complementation of YF NS1 shown by Linden- bach and Rice (18, 19) when providing individually expressed NS5 and NS1 proteins, ...with complementation of YF NS1 were ... See full document
9
NS2 is dispensable for efficient assembly of hepatitis C virus-like particles in a bipartite trans-encapsidation system.
... HCV structural proteins, C-p7, in place of luciferase or NPT in the first ...HCV RNA, which would otherwise ultimately be destined to be packaged, this appears to prevent the ability of those same ... See full document
15
Encapsidation of poliovirus replicons encoding the complete human immunodeficiency virus type 1 gag gene by using a complementation system which provides the P1 capsid protein in trans.
... in trans from a recombinant vaccinia virus, VV-P1, to encapsidate these genomes (22, 24, ...of virus particles containing the replicon ...this system to further define the role that the ... See full document
8
Efficient trans-Encapsidation of Hepatitis C Virus RNAs into Infectious Virus-Like Particles
... plus-strand RNA viruses, HCV induces intracellular membrane alterations and repli- cates its genome in conjunction with vesicular membrane struc- tures, the so-called “membranous web” (10, ...in RNA ... See full document
13
trans-Complementation of Flavivirus RNA Polymerase Gene NS5 by Using Kunjin Virus Replicon-Expressing BHK Cells
... predicted RNA polymerase motif GDD (producing FLdGDD) and of one of the predicted methyltransferase motifs (S-adenosylmethionine [SAM] binding site, producing FLdSAM) were introduced separately into ...An ... See full document
10
Efficient Rescue of Hepatitis C Virus RNA Replication by trans-Complementation with Nonstructural Protein 5A
... for trans-complementation of the pestivirus BVDV ...by trans-complemen- tation. However, in that study complementation only was pos- sible when mutants were transfected into cell clones that ... See full document
Replicon System for Lassa Virus
... T7 RNA polymerase supports transcription and replication of the ...recombinant virus were reported previously in the establishment of reverse genetic systems for filoviruses ...transcribing RNA from ... See full document
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cis- and trans-Acting Elements in Flavivirus RNA Replication
... complemented RNA from day 2 to day 4 after transfection was detected by Northern blot analysis ...complemented RNA from days 4 to 6 after transfection (4d in ...complemented RNA later in transfection ... See full document
11
trans-Complementation of yellow fever virus NS1 reveals a role in early RNA replication.
... viral RNA in the absence of NS1 ...wild-type virus appears to begin very soon after infection, since a minor increase is seen even by 3 ...of complementation, this virus failed to show a ... See full document
10
Development of a foot and mouth disease virus replicon system for the study of RNA replication
... T7 RNA polymerase has been described (Du et ...E.coli RNA polymerase and form stem-loop structures in the nascent RNA, causing the polymerase to disengage from the template strand, halting ...that ... See full document
201
Functional complementation of nuclear targeting-defective mutants of simian virus 40 structural proteins.
... Since what was observed in host cells during infection by polyomaviruses 10, 13, 19, 22, 23, 26 does not reveal the role of individual protein components for nuclear targeting, these obs[r] ... See full document
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Inhibition of Interferon Signaling by the New York 99 Strain and Kunjin Subtype of West Nile Virus Involves Blockage of STAT1 and STAT2 Activation by Nonstructural Proteins
... The interferons (IFNs) are a large family of multifunctional secreted cytokines involved in antiviral defense, cell growth regulation, and immune activation. IFNs are produced by the majority of cells and include 14 ... See full document
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cis-active structural motifs involved in specific encapsidation of Moloney murine leukemia virus RNA.
... or virus RNAs from M-MuLV- infected 3T3 (lanes 3 and 12) or untransfected Psi2 (lanes 4, 11, 14, and 23) ...neo RNA were detected with all cellular RNAs from stably trans- fected Psi2 cells (lanes 8 ... See full document
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The Naturally Attenuated Kunjin Strain of West Nile Virus Shows Enhanced Sensitivity to the Host Type I Interferon Response
... WNV virus in the spleen (E to H) and brain (I to L) were determined from samples harvested on the indicated days using quantitative reverse transcription-PCR (qRT-PCR; serum) or viral plaque assay (spleen ... See full document
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Structural Properties of the C Terminus of Vesicular Stomatitis Virus N Protein Dictate N-RNA Complex Assembly, Encapsidation, and RNA Synthesis
... VSV replicon construction. A VSV replicon plasmid encoding en- hanced green fluorescent protein (eGFP) was prepared with the backbone vector ...by using a previously described cloning strategy to ... See full document
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