Top PDF Enhancement of Autophagy during Lytic Replication by the Kaposi's Sarcoma-Associated Herpesvirus Replication and Transcription Activator
Enhancement of Autophagy during Lytic Replication by the Kaposi's Sarcoma-Associated Herpesvirus Replication and Transcription Activator
... of autophagy is not an overexpression phenotype, a car- boxyl-terminal truncated RTA plasmid (mRTA) was overex- pressed in the 293T cells for GFP dot ...of autophagy, 3-MA ...
11
Kaposi's Sarcoma-Associated Herpesvirus Utilizes and Manipulates RNA N6-Adenosine Methylation To Promote Lytic Replication
... might block RTA pre-mRNA splicing. To test this hypothesis, we converted the RNAs for which the results are described in Fig. 7 into cDNAs using an ORF50 (RTA) transcript- specific primer (Fig. 8A), followed by qRT-PCR ...
21
Development of an ORF45-Derived Peptide To Inhibit the Sustained RSK Activation and Lytic Replication of Kaposi's Sarcoma-Associated Herpesvirus
... The lytic replication of Kaposi’s sarcoma-associated herpesvirus (KSHV) requires sustained extracellular signal-regulated kinase (ERK)-p90 ribosomal S6 kinase (RSK) activation, which is ...
13
Epstein-Barr Virus Inhibits Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication in Primary Effusion Lymphomas
... induce lytic viral ...Reverse transcription was performed with the use of the SuperScript reverse transcription system from Invitrogen, following the manufacturer’s recommended ...30 s, 62°C ...
11
Kaposi's Sarcoma-Associated Herpesvirus Transactivator RTA Promotes Degradation of the Repressors To Regulate Viral Lytic Replication
... Kaposi’s sarcoma-associated herpesvirus (KSHV)/human herpesvirus 8 (HHV-8) is a recently discovered human gam- maherpesvirus that is frequently found in AIDS patients ...Kaposi’s ...
14
Virus-Like Vesicles of Kaposi's Sarcoma-Associated Herpesvirus Activate Lytic Replication by Triggering Differentiation Signaling
... Kaposi’s sarcoma-associated herpesvirus (KSHV) mutant that is defective in small capsid protein and is unable to produce mature vi- ...ing lytic replication, but lacked capsid ...viral ...
18
Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 Replication and Transcription Activator Regulates Viral and Cellular Genes via Interferon-Stimulated Response Elements
... RTA K152E failed to activate RTA-responsive genes. Since RTA K152E has a reduced ability to bind to DNA, we tested its ability to activate RTA-responsive promoter constructs. RTA K152E was unable to activate several ...
13
Glycolysis, Glutaminolysis, and Fatty Acid Synthesis Are Required for Distinct Stages of Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication
... Virus. Titers of KSHV inocula from induced BCBL-1 cells were determined, and the inocula were used to infect TIME cells as previously described (36). Adenoviruses expressing the lytic replication and ...
15
Hsp70 Isoforms Are Essential for the Formation of Kaposi's Sarcoma-Associated Herpesvirus Replication and Transcription Compartments
... ’ s sarcoma-associated herpesvirus (KSHV) is an oncogenic herpesvirus associated with various AIDS-related ...KSHV lytic replication, including viral ...
42
Identification of the Essential Role of Viral Bcl-2 for Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication
... Kaposi’s sarcoma-associated herpesvirus (KSHV) evades host defenses through tight suppression of autophagy by targeting each step of its signal transduction: by viral Bcl-2 (vBcl-2) in vesicle ...
10
Caspase-Dependent Suppression of Type I Interferon Signaling Promotes Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication
... induction during KSHV lytic reac- tivation, we treated reactivated cells with the caspase-3/7 selective inhibitor Z-DEVD- FMK, the caspase-8 selective inhibitor Z-IETD-FMK, or the caspase-1 selective ...
17
Inhibition of Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication by HIV-1 Nef and Cellular MicroRNA hsa-miR-1258
... ated with immunosuppression (6). Immunosuppression clearly plays an important role in the development of AIDS-KS; however, it cannot explain the following problems. First, an elevated inci- dence of KS in patients with ...
14
Acetylation of the Latency-Associated Nuclear Antigen Regulates Repression of Kaposi's Sarcoma-Associated Herpesvirus Lytic Transcription
... and transcription factor Sp1 (4, 32, ...Sp1 associated with LANA in untreated BCBL1 cells but not in cell extracts that were pretreated with NaB for 6 h, a time too short to induce lytic ...
10
Transcriptional Downregulation of ORF50/Rta by Methotrexate Inhibits the Switch of Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus 8 from Latency to Lytic Replication
... the lytic rather than the latent cycle, and among them, foscarnet and cidofovir have the best activity against KSHV (27, ...early lytic gene ...early lytic genes are transcribed before viral DNA ...
12
β-Adrenoreceptors Reactivate Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication via PKA-Dependent Control of Viral RTA
... trans-activational enhancement by both PKA and PKC, it remained a functional transcription factor as shown by its capacity to induce the PAN promoter by an av- ...Posttranslational enhancement of RTA ...
10
The Human Cytomegalovirus UL112-113 Locus Can Activate the Full Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication Cycle
... KSHV lytic gene expression by HCMV in a number of these cell types, including human fibroblasts (HF), human umbilical cord endothelial cells, and keratinocytes, has been demonstrated ...KSHV lytic gene ...
5
Kaposi's Sarcoma-Associated Herpesvirus K3 and K5 Ubiquitin E3 Ligases Have Stage-Specific Immune Evasion Roles during Lytic Replication
... Notch-induced transcription factor RBP-J (39, 85, ...RTA during latency (87, ...the transcription start site (TSS) of K3 (40, 56), raising questions about the mechanism by which vIRF1 might regulate ...
15
Kaposi's Sarcoma-Associated Herpesvirus-Encoded Replication and Transcription Activator Impairs Innate Immunity via Ubiquitin-Mediated Degradation of Myeloid Differentiation Factor 88
... I nnate immunity is triggered by pattern recognition receptors, including the Nod-like receptors, RIG-like receptors, Toll-like receptors (TLRs), and cytosolic DNA receptors (1–4). Innate im- munity is the first-line ...
13
Evaluation of the Lytic Origins of Replication of Kaposi's Sarcoma-Associated Virus/Human Herpesvirus 8 in the Context of the Viral Genome
... that lytic repli- cation of human herpesvirus 8 (HHV8) is directed by two origins of DNA replication, oriLyt-L and oriLyt-R (2, ...these lytic origins play essential or ancillary roles in the ...
5
A Virally Encoded Small Peptide Regulates RTA Stability and Facilitates Kaposi's Sarcoma-Associated Herpesvirus Lytic Replication
... extensive transcription from noncoding regions, including both intergenic regions and noncoding regions antisense to known open reading frames (ORFs) ...be associated with these noncoding ...
10
