[PDF] Top 20 The Enigma of Double-stranded RNA (dsRNA) Associated with Mushroom Virus X (MVX)
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The Enigma of Double-stranded RNA (dsRNA) Associated with Mushroom Virus X (MVX)
... origin. Double-stranded RNAs, some in non-encapsidated forms are increasingly becoming causal agents for sporadic diseases and are consistently associated with a complex profile of dsRNAs, presumably of ... See full document
20
Double-Stranded RNA-Mediated Interference with Plant Virus Infection
... viral RNA penetrates into the cell in combination with ...viral RNA for deg- ...54-kDa-protein RNA-expressing vector, or a mixture of both ...PMMoV RNA was assessed on inoculated (lanes 1 to ... See full document
10
In vitro selection of packaging sites in a double-stranded RNA virus.
... Packaging and replication. Most of the selected VBS se- quences were tested for incorporation into viral particles and for replication within viral particles (synthesis of minus strand). Cells lacking any ScVLa viral ... See full document
6
Comparison of Polymerase Subunits from Double-Stranded RNA Bacteriophages
... the same time, show a higher thermostability (Fig. 5). Most importantly, the purified enzymes have different tem- plate specificities, for both ssRNAs and dsRNAs (Fig. 6 to 8). Several generalizations are appropriate in ... See full document
8
Sequestration and Protection of Double-Stranded RNA by the Betanodavirus B2 Protein
... total RNA was prepared from the transfected cells using the Trizol reagent (Invitrogen) and treated with RQ1 RNase-free DNase (Promega) and the editing substrates were amplified by StrataScript reverse ... See full document
12
Polioviruses, Coxsackieviruses, and Echoviruses: Comparison of the Genomes by RNA Hybridization
... The methods for extraction and purification of viral double-stranded RNA dsRNA from infected cells by treatment with hot phenol, differential salt preparation of singlestranded RNA ssRNA[r] ... See full document
8
Nonspecific Nucleoside Triphosphatase P4 of Double-Stranded RNA Bacteriophage φ6 Is Required for Single-Stranded RNA Packaging and Transcription
... segmented double-stranded RNA genome. The genomic single-stranded RNA (ssRNA) precursors are packaged into a preformed protein capsid, the polymerase complex, composed of viral proteins ... See full document
6
Double-stranded RNA-activated protein kinase PKR of fishes and amphibians: Varying the number of double-stranded RNA binding domains and lineage-specific duplications
... In addition to the early duplication leading to fish PKZ and PKR genes, four independent duplication events are evident. Two of these duplications can be observed in T. nigroviridis: first, an early duplication that gave ... See full document
19
Visualization of Double-Stranded RNA in Cells Supporting Hepatitis C Virus RNA Replication
... HCV RNA synthesis, in which genome replication occurs within ER-derived vesicles of the membranous web whose formation is believed to be driven, at least in part, by the oligomerization of NS4B (10, 15, ...HCV ... See full document
14
A Kinome-Wide Small Interfering RNA Screen Identifies Proviral and Antiviral Host Factors in Severe Acute Respiratory Syndrome Coronavirus Replication, Including Double-Stranded RNA-Activated Protein Kinase and Early Secretory Pathway Proteins
... Coronavirus replication is associated with a cytoplasmic re- ticulovesicular network of modified ER, including double-mem- brane vesicles and convoluted membranes (28). Despite the in- depth characterization of ... See full document
16
RNA-Binding Activity of the Rotavirus Phosphoprotein NSP5 Includes Affinity for Double-Stranded RNA
... NSP5 is a dimeric (39), serine/threonine-rich protein of 198 amino acids that is slightly acidic and undergoes O-linked glycosylation (15) and phosphorylation in infected cells (2, 5). Due to variations in the extent of ... See full document
9
The RNA-Binding Protein of a Double-Stranded RNA Virus Acts like a Scaffold Protein
... The similarity of the CP fold and virion architecture within the same viral lineage entails the additional resemblance of structural components related to virion assembly (6) and genome packaging (7). Minor or transient ... See full document
14
[HOK], A NEW YEAST NON-MENDELIAN TRAIT, ENABLES A REPLICATION-DEFECTIVE KILLER PLASMID TO BE MAINTAINED
... Chromosomal genes essential for replication of a double-stranded RNA plasmid of Saccharomyces cereuisiae: the killer character of yeast. A comparison of the killer ch[r] ... See full document
16
Infectious Bursal Disease Virus Capsid Protein VP3 Interacts both with VP1, the RNA-Dependent RNA Polymerase, and with Viral Double-Stranded RNA
... VP3 interacts both with VP1 and with IBDV-specific dsRNA. To study the VP1-VP3 interaction per se, i.e., without the interference of RNA-mediated coimmunoprecipitation, we performed an RNase treatment on all the ... See full document
11
Homology between double-stranded RNA and nuclear DNA of yeast.
... Virus-like particles associated with the double-stranded RNA species found in killer and sensitive strains of the yeast Saccharomyces cervisiae.. Expression of the mitochondrial genome i[r] ... See full document
6
Heterologous recombination in the double-stranded RNA bacteriophage phi 6.
... Virus from a single plaque contained from about 0.1 to 10%/Y large clear-plaque forms of the virus; these were usually missing the kanamycin resistance gene, and in many cases, the resul[r] ... See full document
6
Structure of a VP1-VP3 Complex Suggests How Birnaviruses Package the VP1 Polymerase
... Infectious pancreatic necrosis virus (IPNV), a member of the family Birnaviridae, infects young salmon, with a severe impact on the commercial sea farming industry. Of the five mature proteins encoded by the IPNV genome, ... See full document
8
A deletion mutant of L-A double-stranded RNA replicates like M1 double-stranded RNA.
... Ethidium bromide-stained agarose gel A and autoradiogram B of the dsRNAs contained in purified VLPs from strain RE455 by cesium chloride density gradient centrifugation and the products [r] ... See full document
8
Separation of Ten Reovirus Genome Segments by Polyacrylamide Gel Electrophoresis
... Double-stranded RNA stranded RNA segments obtained from all three purified from reovirus type 3 was separated into reovirus serotypes have been resolved further by isolated distinct band[r] ... See full document
6
Electron Microscopy of the Nucleic Acid of Mouse Mammary Tumor Virus
... Some single-stranded RNA molecules have characteristics which distinguish them prepared from bacteriophage R17 and the known from double-stranded RNA molecules 11, molecular weight, Gran[r] ... See full document
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