[PDF] Top 20 Evaluating the Adsorbed Water Layer on Polar Stationary Phases for Hydrophilic Interaction Chromatography (HILIC)

... the polar stationary phases selected for this study were either purchased from or kindly donated by the column ...the stationary phases including stationary phase chemistry, ... See full document

... Hydrophilic interaction liquid chromatography (HILIC) is becoming increasingly popular for the separation of polar or ionised ...In HILIC, retention is thought to occur ... See full document

... Hydrophilic interaction liquid chromatography (HILIC) is fast becoming an established tool for the separation of polar and/or ionised ...methods. Stationary phases in ... See full document

... term,‟Hydrophilic interaction chromatography” in 1990. This mode of chromatography dates back to earliest days of liquid ...column. Water saturated chloroform as mobile phase was used ... See full document

... four HILIC columns of substantially different ...mobile phases in terms of retention or column efficiency for the neutral ...on stationary phases with strong ionic retention characteristics ... See full document

... liquid chromatography (UPLC) with negative electrospray ioniza- tion mass spectrometry (MS) with the aid of synthesized organosulfate ...drophilic interaction liquid chromatography (HILIC) ... See full document

... The comprehensive understanding of the role of those chemical entities used on the preparation of MIPs is a very demanding task [7]. Attending to the literature some remarks could be considered: the relevance of the ... See full document

... modern stationary phases were developed with different optimized mobile phase composition and gradient elution to achieve efficient separation of all eight ... See full document

... of water masses and interactions with ambient water from the continental slope and the tidewater glacier ...subsurface water is associated with the Pacific water outflow from the Arctic ... See full document

... mobile phases it has limitations based on pH ...of stationary phase stability is to fundamentally alter this bonding reaction and to produce a direct silicon-carbon bond at the ... See full document

... causes water to bead up on the surface, and pick up the contaminants as it rolls off the surface, and thus prevent attachment of any cells onto the surface [11, ...of water, sloughs off the organism of the ... See full document

... As expected, the bulk of peptides could be observed in the flow-through fractions from 1 min to 12 min (see Fig. 1). They comprise non-glycosylated peptides, whereas high abundant proteins such as glycoprotein IIb/IIIa ... See full document

... via hydrophilic interaction liquid chromatography with fluorescence detection (HILIC-fluorescence) provided evidence of predominantly high mannose glycans (M2–9), with the occurrence of ... See full document

... of chromatography are defined essentially according to the nature of the interactions between the solute and the stationary phase, which may arise from hydrogen bonding, Vander walls forces, electrostatic ... See full document

... the competing effects of the expanding interface on the one hand and equilibration of bulk and interface concentration through adsorption on the other hand. For an initially surfactant free interface, the droplet wets ... See full document

... Stability is an important and essential aspect of validation in thin layer chromatographic analysis. Highly sensitive samples should not decompose during development of the chromatogram and should be stable in ... See full document

... values for BSA, 2-NSA and p-XSA are relatively modest (-1.5, -0.5, -0.8 respectively). The experiments were repeated using columns from a different manufacturer (Agilent Glycan amide and bare silica columns) in order to ... See full document

... ionisable functional groups into the sugars thereby both improving separation and the detection of the poorly ionising sugars by electrospray mass spectrometry [7-19]. A frequently adopted strategy for sugar ... See full document

... analysis. Chromatography is based on principle where the mixture of substances is separated according to their affinity towards stationary phase and mobile phase is used for elution of separated ... See full document

... crystal stationary phases according to their polarities, also, it is investigate the correlation between the molecular structure and the polarities of these liquid crystals towards each type of ... See full document