[PDF] Top 20 Evaluation of Aspergillus PCR Protocols for Testing Serum Specimens
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Evaluation of Aspergillus PCR Protocols for Testing Serum Specimens
... human serum samples spiked with various amounts of Aspergillus fumigatus genomic DNA was distributed to 23 centers within the European Aspergillus PCR Initiative to determine analytical ... See full document
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Evaluation of Analytical and Preliminary Clinical Performance of Myconostica MycAssay Aspergillus When Testing Serum Specimens for Diagnosis of Invasive Aspergillosis
... Aspergillus PCR has been used to test a variety of specimens (bronchoalveolar lavage [BAL] fluid/sputum, serum/plasma, whole blood, and tissue) with various degrees of success, al- though ... See full document
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Analytical Comparison of In Vitro Spiked Human Serum and Plasma for PCR Based Detection of Aspergillus fumigatus DNA: a Study by the European Aspergillus PCR Initiative
... board. Serum and plasma were fraction- ated by centrifugation and pooled, respectively, before being distributed to Public Health Wales, Microbiology Cardiff, Cardiff, United Kingdom, to develop the ...through ... See full document
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A Comparison of Aspergillus and Mucorales PCR Testing of Different Bronchoalveolar Lavage Fluid Fractions from Patients with Suspected Invasive Pulmonary Fungal Disease
... and protocols for the diagnosis of ...5], serum [6], plasma [7], or a combination of them [8]) have prospectively been used to both confirm and exclude IMD using real-time PCR assays amplifying ... See full document
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Molecular Diagnostic Testing for Aspergillus
... clinical specimens have been found to display decreased susceptibility to azole antifungal ...resistance testing has been applied to cultured isolates as a part of epidemiologic drug resistance surveillance ... See full document
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Multicenter Comparison of Serum and Whole Blood Specimens for Detection of Aspergillus DNA in High Risk Hematological Patients
... both serum and WB specimens, there have been only limited comparisons of their ...of Aspergillus in serum and WB specimens ob- tained from patients at high risk for ...by PCR, ... See full document
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Evaluation of the Novel Helicobacter pylori ClariRes Real Time PCR Assay for Detection and Clarithromycin Susceptibility Testing of H pylori in Stool Specimens from Symptomatic Children
... real-time PCR protocols on the basis of biprobes (1, 4) or hybridization probes (13, 22) for the detection of point mutations in the 23S rRNA gene asso- ciated with resistance to clarithromycin has ... See full document
5
Comparison of Performance Characteristics of Aspergillus PCR in Testing a Range of Blood Based Samples in Accordance with International Methodological Recommendations
... European Aspergillus PCR Initiative for the testing of whole blood, serum, and ...of Aspergillus PCR when testing these different sample types has been performed, no ... See full document
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Clinical Performance of Aspergillus PCR for Testing Serum and Plasma: a Study by the European Aspergillus PCR Initiative
... clinical evaluation supports the analytical evidence confirm- ing free DNA to be more abundant in plasma than in ...Plasma PCR sensitivity (94.7%) was higher than that of serum PCR ...to ... See full document
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Comparison of Nonculture Blood Based Tests for Diagnosing Invasive Aspergillosis in an Animal Model
... of Aspergillus in clinical specimens using mo- lecular methods is yet to be included in criteria for defining inva- sive fungal disease (IFD), which is a result of limited standardiza- tion ...European ... See full document
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Optimized Diagnosis of Acute Dengue Fever in Swedish Travelers by a Combination of Reverse Transcription PCR and Immunoglobulin M Detection
... multiplex PCR primers were targeting a differ- ent region in the dengue virus genome (C versus NS-5) of another DEN-1 strain (Hawaii versus West Pac) compared to the DEN-1 TaqMan ...TaqMan PCR were all ... See full document
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Replicate PCR Testing and Probit Analysis for Detection and Quantitation of Chlamydia pneumoniae in Clinical Specimens
... of PCR inhibitors. We suggest adding the issue of PCR replicates to their list of areas where standardization is ...many PCR replicates were done by a lab- oratory and how a positive specimen was ... See full document
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Detection of human immunodeficiency virus type 1 infection in young pediatric patients by using polymerase chain reaction and biotinylated probes
... Polymerase chain reaction PCR testing using up to four primer pairs and biotinylated probes was 97.9% sensitive 188 of 192 specimens positive and 100% specific 267 of 267 specimens negat[r] ... See full document
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Pooling Nasopharyngeal/Throat Swab Specimens To Increase Testing Capacity for Influenza Viruses by PCR
... acid. PCR was performed according to the CDC protocol (24) using the SuperScript III platinum one-step quantitative RT-PCR kit (Invitrogen Corporation, Carlsbad, CA) on the Applied Biosystems (ABI) 7500 ... See full document
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Transfer and Evaluation of an Automated, Low Cost Real Time Reverse Transcription PCR Test for Diagnosis and Monitoring of Human Immunodeficiency Virus Type 1 Infection in a West African Resource Limited Setting
... real-time PCR test provided a significant virologic contribution to the early diagnosis of HIV infection in children, the reliable quantification of non-B HIV-1 subtypes circulating in Co ˆte d’Ivoire, and the ... See full document
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Specific antibody detection in invasive aspergillosis by analytical isoelectrofocusing and immunoblotting methods
... (16 serum specimens) were placed in one of three groups, either proven, highly probable, or probable IA (Table ...positive Aspergillus cultures from biopsy and deep tissue ...positive ... See full document
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Comparison of two different methods for inactivation of viruses in serum.
... compare protocols for inactivation of viruses potentially present in biological specimens, three different model viruses were treated in bovine serum by two different inactivation methods: samples ... See full document
5
Evaluation of Amplicor PCR for direct detection of Mycobacterium tuberculosis from sputum specimens
... Amplicor PCR assay (Roche Molecular Systems, Branchburg, ...532 specimens from 270 patients were decontaminated and stored at 4 or 2 75 & C until assayed by ...Amplicor PCR results were compared ... See full document
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The Effect Analysis of Different Experimental Methods for the Diagnosis of Invasive Pulmonary Aspergillosis in a Rat Model
... of Aspergillus infec- tion have been sought for at least four decades, but in- adequate sensitivity has restricted the general application of many methods ... See full document
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Detection of Circulating Aspergillus fumigatus DNA by Real Time PCR Assay of Large Serum Volumes Improves Early Diagnosis of Invasive Aspergillosis in High Risk Adult Patients under Hematologic Surveillance
... hematological malignancies. Indeed, increasing the starting se- rum volume for DNA extraction from 100 l to 1 ml increased the sensitivity and NPV of the test to 100% without affecting the other performance parameters. ... See full document
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