[PDF] Top 20 Evaluation of Methods for Testing the Susceptibility of Clinical Mycobacterium tuberculosis Isolates to Pyrazinamide
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Evaluation of Methods for Testing the Susceptibility of Clinical Mycobacterium tuberculosis Isolates to Pyrazinamide
... M. tuberculosis cells as the testing well with PZA, was ...M. tuberculosis cells of the testing well with PZA, was also ...M. tuberculosis strains in which the MIC was ⱕ 100 mg/liter ... See full document
7
Direct Susceptibility Testing of Mycobacterium tuberculosis for Pyrazinamide by Use of the Bactec MGIT 960 System
... drug susceptibility testing ...PZA susceptibility testing was performed as described previously by Siddiqi et ...PZA susceptibility testing ... See full document
6
Testing of Susceptibility of Mycobacterium tuberculosis to Pyrazinamide with the Nonradiometric BACTEC MGIT 960 System
... 960 pyrazinamide (PZA) kit (Becton Dickinson Microbiology Systems, Sparks, ...for testing of susceptibility of Mycobacterium tuberculosis to ...proficiency testing (phase I) and ... See full document
5
High Resolution Melting Curve Analysis for Rapid Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis Clinical Isolates
... for tuberculosis (TB) treat- ...M. tuberculosis [4]. The lack of reliable cul- ture-based methods which require an acidic condition and high cost of the molecular techniques makes the testing ... See full document
18
Rapid Detection of Mycobacterium tuberculosis and Pyrazinamide Susceptibility Related to pncA Mutations in Sputum Specimens through an Integrated Gene to Protein Function Approach
... phenotypic methods for testing PZA susceptibility of M. tuberculosis, such as their lengthiness, high cost and low accuracy, DNA sequencing of the pncA gene is being used more and ...for ... See full document
8
Evaluation of Etest for Susceptibility Testing of Multidrug Resistant Isolates of Mycobacterium tuberculosis
... Tuberculosis (TB) is a growing global health problem, both in terms of disease burden and in terms of resistance to con- ventional chemotherapy (7). The regions where TB is more prevalent lack the resources to ... See full document
5
Evaluation of the BACTEC radiometric method for detection of 1% resistant populations of Mycobacterium tuberculosis
... BACTEC and conventional methods of antimicrobial susceptibility testing were compared with the use of artificial mixtures of 1% resistant and 99% susceptible Mycobacterium tuberculosis s[r] ... See full document
6
Susceptibility Testing for the Polymyxins: Two Steps Back, Three Steps Forward?
... standardizing susceptibility testing for the polymyxins have become pressing issues, given the rise in multidrug-resistant Gram-negative ...Enterobacteriaceae isolates (21 of which were mcr-1 ...this ... See full document
10
Photographic and Luminometric Detection of Luciferase Reporter Phages for Drug Susceptibility Testing of Clinical Mycobacterium tuberculosis Isolates
... of tuberculosis (TB) and drug-resistant TB allows the prescription of appropriate antibiotic regimens, leading to more efficient control of the disease ...drug susceptibility techniques ...M. ... See full document
5
Pyrazinamide Resistance among South African Multidrug Resistant Mycobacterium tuberculosis Isolates
... and pyrazinamide-susceptible isolates of M. tuberculosis have established an association between mutations in this gene and pyrazinamide ...resistant isolates which have a wild-type ... See full document
6
Multicenter Laboratory Evaluation of the MB/BacT Mycobacterium Detection System and the BACTEC MGIT 960 System in Comparison with the BACTEC 460TB System for Susceptibility Testing of Mycobacterium tuberculosis
... evaluated methods were re- solved by means of reanalysis with the two methods (12) or with the proportion method on Lo ¨wenstein-Jensen slants (3–5, 22) or in Middlebrook 7H11 agar ...MB/BacT ... See full document
5
Evaluation of Colorimetric Methods Using Nicotinamide for Rapid Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis
... macrophages, pyrazinamide (PZA) is one of the most effect frontline drugs used in the short-course chemotherapy of tuberculosis (TB) and also in retreatment regimens for multi- drug-resistant TB patients ... See full document
5
Genotypic Susceptibility Testing of Mycobacterium tuberculosis Isolates for Amikacin and Kanamycin Resistance by Use of a Rapid Sloppy Molecular Beacon Based Assay Identifies More Cases of Low Level Drug Resistance than Phenotypic Lowenstein Jensen Testing
... phenotypic susceptibility test methods principally involved isolates with eis promoter muta- ...MIC testing to more care- fully explore the relationship between rrs and eis promoter muta- ... See full document
9
Current Perspectives on Drug Susceptibility Testing of Mycobacterium tuberculosis Complex: the Automated Nonradiometric Systems
... broth-based methods with a shortened incubation time were introduced into clinical laboratories to fulfill CDC ...of susceptibility to first- line ...the Clinical and Laboratory Standards ... See full document
9
Discordance across Several Methods for Drug Susceptibility Testing of Drug Resistant Mycobacterium tuberculosis Isolates in a Single Laboratory
... quantitative susceptibility method for MDR-TB detection, such as the Trek MycoTB plate or the phage qPCR ...these methods were readily established in the laboratory, provided information for a broad range ... See full document
8
Direct Detection of Pyrazinamide Resistance in Mycobacterium tuberculosis by Use of pncA PCR Sequencing
... in clinical strains in our current study, it is still important to consider the potential occurrence of pncA mutations in phenotypic PZA-susceptible ...the clinical setting in our ...MDR-TB isolates ... See full document
10
Targeted Drug Identification and Susceptibility Testing of Typical Mycobacterium Tuberculosis Directly from Versa Trek Mycobottles with Trouble Shooting Approac
... Although clinical trials and experiments involving whole animals are important in natural product screening but because of financial, ethical and time limitations, importance of invitro screening is gaining ... See full document
15
Mycobacterium tuberculosis Pyrazinamide Resistance Determinants: a Multicenter Study
... M. tuberculosis complex. Strains were tested for PZA susceptibility and included in the study regardless of testing for other antitubercular ...drug susceptibility testing (DST) was ... See full document
10
Sequencing of the pncA Gene in Members of the Mycobacterium tuberculosis Complex Has Important Diagnostic Applications: Identification of a Species Specific pncA Mutation in “Mycobacterium canettii” and the Reliable and Rapid Predictor of Pyrazinamide Resistance
... ceptibilities to PZA has been complicated by the fact that this drug is active only at acidic pH; a significant number of M. tuberculosis isolates would not grow at pH 5.5 in conventional solid media (4, ... See full document
5
Polymorphisms Associated with Resistance and Cross Resistance to Aminoglycosides and Capreomycin in Mycobacterium tuberculosis Isolates from South Korean Patients with Drug Resistant Tuberculosis
... (68%) isolates were resistant to both INH and RIF (MDR); among these, 18 isolates were pre-XDR (being resistant to either KM or a fluoroquinolone but not both) and 14 (13%) fit the formal definition of XDR ... See full document
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