[PDF] Top 20 Evidence for direct involvement of T7 RNA polymerase bacteriophage DNA replication.
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Evidence for direct involvement of T7 RNA polymerase bacteriophage DNA replication.
... The experiments scripts are used as primers for DNA synthesis indicated that the inactivation of the gene 1 18, 19, In other cases this has not yet been RNA polymerase caused the cessati[r] ... See full document
6
A Complex of the Bacteriophage T7 Primase-Helicase and DNA Polymerase Directs Primer Utilization
... the replication fork moves along a DNA duplex, DNA primase periodically deposits short RNA primers at specific priming sequences on the lagging strand, triggering the syn- thesis of Okazaki ... See full document
14
Direct removal of RNA polymerase barriers to replication by accessory replicative helicases
... full-length replication products. Thus accessory helicase action during replication-transcription collisions promotes continued replication without leaving gaps in the ...with evidence that ... See full document
27
An Interaction between DNA Polymerase and Helicase Is Essential for the High Processivity of the Bacteriophage T7 Replisome
... the polymerase is maintained during leading strand synthe- ...strand DNA. gp5/trx is recruited to the replication fork by an initial interaction with the acidic C-terminal tail of gp4 through its ... See full document
13
Involvement of DNA gyrase in replication and transcription of bacteriophage T7 DNA.
... This is particularly true of RNA synthesis, where only late transcription appears to be inhibited by coumermycin; but with DNA synthesis, which is more completely inhibited by the antibi[r] ... See full document
7
Virus-specific cytotoxic T-lymphocyte activity elicited by coimmunization with human immunodeficiency virus type 1 genes regulated by the bacteriophage T7 promoter and T7 RNA polymerase protein.
... recombinant T7 RNAP are thought to amplify expression of genes controlled by the T7 promoter through an autocrine mechanism (3, 5) and thus were evaluated as a substitute for the VTF7-3 ...and T7 ... See full document
5
Direct removal of RNA polymerase barriers to replication by accessory replicative helicases
... represent +/- one standard error of four experiments. (C) Denaturing agarose gel showing a time course of replication products when RNA polymerase is stalled on pPM872 in the absence of CTP. ... See full document
13
DNA packaging in vitro by an isolated bacteriophage T7 procapsid.
... It was shown here that capsid I, isolated by electrophoresis in metrizamide density gradients, packaged DNA and formed infectious phage particles when incubated in vitro with extracts de[r] ... See full document
5
Involvement of the bacterial groM gene product in bacteriophage T7 reproduction. I. Arrest at the level of DNA packaging.
... 9 and 10 and analyzed by electron microscopy Infected host BE K-12-M Fraction 16 30 35 50 16 31, 34 45 50 54 Observed structures Phages DNA capsid complexes, prehead II, free DNA Prehead[r] ... See full document
17
Bacillus subtilis DNA Polymerase III Is Required for the Replication of the Virulent Bacteriophage φe
... The immediate arrest of phage-DNA synthesis in oe-infected 2355pol IIP' cells upon transfer from 37 to 48 C indicates that DNA polymerase III is required for phage-DNA synthesis througho[r] ... See full document
6
Amino Acid Residues Critical for the Interaction between Bacteriophage T7 DNA Polymerase and Escherichia coli Thioredoxin
... of T7 gene 5 protein bound to DNA based on the structure of an ...coli DNA polymerase I Klenow fragment-DNA ...coli DNA polymerase I bound to DNA was determined by ... See full document
12
Purification and structures of recombining and replicating bacteriophage T7 DNA.
... This structure was seen mn at 15 in a sample of EcoRI treated intracellular DNA taken after infection and sedimenting faster than unit-sized phage DNA.. The region at c is therefore undo[r] ... See full document
8
A Single-stranded DNA-binding Protein of Bacteriophage T7 Defective in DNA Annealing
... for T7 survival. DNA annealing is important in both DNA repair and ...age T7 has a high rate of recombination, and mutations in gene ...the T7 chromosome without subsequent annealing to ... See full document
10
Regulated expression of foreign genes in vaccinia virus under the control of bacteriophage T7 RNA polymerase and the Escherichia coli lac repressor.
... FUERST1* Department of Molecular Genetics, MedImmune Inc., Gaithersburg, Maryland 20878,1 and Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesd[r] ... See full document
9
RNA Polymerase Activity Associated with Bacteriophage φ6
... Experiments to determine if the radioactivity incorporated in the assay mixture was associated with the phage particle could not be conducted since the short heat treatment used to stimu[r] ... See full document
8
The Template Specificity of Bacteriophage Φ6 RNA Polymerase
... double-stranded RNA (dsRNA) genomic segments, L, M, and S. The RNA is located inside a core particle composed of multiple copies of a major structural protein, an RNA-dependent RNA ... See full document
5
Role of gene 2 in bacteriophage T7 DNA synthesis.
... It was observed that after infection with T7 wild type a major portion of the newly synthesized DNA sedimented in both alkaline and neutral sucrose gradients 1.3 to 1.6 times as fast as [r] ... See full document
7
Electron microscopic analysis of partially replicated bacteriophage T7 DNA.
... 45[Suppl.]:51-74, 1955 as an explanation for the distribution of marker rescue frequencies during cross-reactivation; i.e., replication proceeds in a bidirectional manner from an origin [r] ... See full document
8
In vitro recombination of bacteriophage T7 DNA damaged by UV radiation.
... DNA from T7%8 phage was irradiated and incubated with extracts from T73- or T73,6-infected strain W3110 under conditions that would permit both replication and recombination.. Controls n[r] ... See full document
10
Revised transcription map of the late region of bacteriophage T7 DNA.
... Through the use of an agarose-formaldehyde gel system that permits the resolution of high-molecular-weight RNAs, transcription of template DNA that had been cut with various restriction [r] ... See full document
7
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