[PDF] Top 20 Expression of the α7 nicotinic acetylcholine receptor in human lung cells
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Expression of the α7 nicotinic acetylcholine receptor in human lung cells
... all cells and tissues. Expression of the α7 receptor by RT-PCR in fetal hamster PNECs has been previously published ...new human α7 prim- ers (forward 5'-gccaatgactcgcaaccactc-3' ... See full document
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α7 Nicotinic Acetylcholine Receptor (α7nAChR) Expression in Bone Marrow-Derived Non-T Cells Is Required for the Inflammatory Reflex
... T cells are necessary for the functional integrity of the inflamma- tory reflex ...T cells as pivotal for their activa- tion to produce acetylcholine, but it re- mained theoretically possible that ... See full document
5
A Human-Specific α7-Nicotinic Acetylcholine Receptor Gene in Human Leukocytes: Identification, Regulation and the Consequences of CHRFAM7A Expression
... analyses, cells were washed and fixed with Cytofix ac- cording to the manufacturer’s recom- mendations (BD Biosciences) for 10 min on ...ice. Cells were then incubated with la- beled bungarotoxin (BD ... See full document
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Activation of α7 nicotinic acetylcholine receptor by nicotine selectively up-regulates cyclooxygenase-2 and prostaglandin E2 in rat microglial cultures
... bungarotoxin. Cells were washed 3 times with BME medium and then fixed with 4% paraformaldehyde at room temperature for 15 ...microglial cells and PC12 cells (used as positive control) were analyzed ... See full document
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Small changes in bone structure of female α7 nicotinic acetylcholine receptor knockout mice
... differentiation and bone degradation [28] the reduction of nicotinic receptors in α7KO might provoke the down-regulation of cathepsin K expression. In male α7KO we did not investigate cathepsin K gene ... See full document
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Imaging of α7 nicotinic acetylcholine receptors in brain and cerebral vasculature of juvenile pigs with [18F]NS14490
... homomeric α7 nicotinic acetylcholine receptor (α7 nAChR), together with the heteromeric α4β2 nAChR, belongs to the cysteine-loop family of ligand-gated ion channels and they represent ... See full document
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Deficiency of α7 Nicotinic Acetylcholine Receptor Attenuates Bleomycin-Induced Lung Fibrosis in Mice
... of α7 nAChR could boost TGF-β1 signaling and tran- scription of fibrogenic ...rhTGF-β1–triggered expression of p-PTP1B, p-Samd2/3, collagen 1 and α-SMA (Figure ...that α7 nAChR is an important reg- ... See full document
16
Prenatal nicotine increases pulmonary α7 nicotinic receptor expression and alters fetal lung development in monkeys
... 32-week-old human fetus and are in the late saccular/early alveolar phase (24, ...both lung maturation and ...decreased lung volume, suggest that nicotine exposure may result in larger but fewer ... See full document
12
Modulation of TNF Release by Choline Requires α7 Subunit Nicotinic Acetylcholine Receptor-Mediated Signaling
... subunit-containing nicotinic acetylcholine receptor ( α 7nAChR) is an essential component in the vagus nerve-based cholinergic anti-inflammatory pathway that regulates the levels of TNF, high ... See full document
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The α7 Nicotinic Acetylcholine Receptor Agonist GTS-21 Improves Bacterial Clearance in Mice by Restoring Hyperoxia-Compromised Macrophage Function
... of lung cells in these mice, nuclear and cytoplas- mic extract was prepared by using the NE-PER Nuclear and Cytoplasmic Extrac- tion Reagents kit (Thermo Fisher Scien- tific, Waltham, MA, USA), according to ... See full document
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Role of a7-nicotinic acetylcholine receptor in nicotine-induced invasion and epithelial-to-mesenchymal transition in human non-small cell lung cancer cells
... the cells on the membrane for 15 min at room ...residual cells on the upper side. Cells migrating to the lower surface of the polycarbonate membrane were stained with ... See full document
10
Nicotine stimulates collagen type I expression in lung via α7 nicotinic acetylcholine receptors
... in lung without affecting the organ’s overall ...in lung architecture, how then do these subtle changes in lung matrix composition affect the lung? We reasoned that newly deposited colla- gen ... See full document
12
Host cell-specific folding of the neuronal nicotinic acetylcholine receptor α7 and α8 subunits
... L929 cells stably transfected with pMSG-a7, an alternative stable expression vector, pSVK3, was used (see Figure ...although expression of cloned gene products can be enhanced by the addition of ... See full document
225
PKCε stimulates vesicular exocytosis of α7 nicotinic ach receptor by targeting nsf
... NSF/α7 nicotinic ACh receptor in a PKCε-dependent ...12 cells were transfected with the negative control (NC) siRNA or the PKCε siRNA, and 48 h later Western blotting was carried out in the ... See full document
7
The assembly and folding of neuronal nicotinic acetylcholine receptors
... The Torpedo nAChR is the most thoroughly characterised receptor in the superfamily of ligand-gated ion channels. The nAChR is an integral membrane glycoprotein of approximately 250 kD. It is a pentameric complex ... See full document
226
Nicotinic receptors on rat alveolar macrophages dampen ATP-induced increase in cytosolic calcium concentration
... Total RNA was isolated from BAL cells (n = 7 rats and n = 5 C57BL6N mice) using RNeasy Mini Kit (Qiagen, Hilden, Germany). Genomic DNA contaminations were removed by DNase I digestion for 15 min at 37°C, 1 U/ ... See full document
16
A susceptibility locus for lung cancer maps to nicotinic acetylcholine receptor subunit genes on 15q25
... the nicotinic acetylcholine receptor subunits CHRNA5, CHRNA3 and CHRNB4, the IREB2 iron- sensing response element, PSMA4, which is implicated in DNA repair, and LOC123688, a gene of unknown function ... See full document
12
Alpha7 nicotinic acetylcholine receptor is required for blood brain barrier injury related CNS disorders caused by Cryptococcus neoformans and HIV 1 associated comorbidity factors
... Abbreviations AIDS: Acquired immune deficiency syndrome; α7 nAChR: Alpha7 nicotinic acetylcholine receptor; BBB: Blood-brain barrier; BMEC: Brain microvascular endothelial Cells; CAPE: C[r] ... See full document
11
Downregulation of α7 nicotinic acetylcholine receptors in peripheral blood monocytes is associated with enhanced inflammation in preeclampsia
... pathway. Furthermore, we found that the α7nAChR expres- sion levels were closely correlated with the severity of pre- eclampsia in terms of blood pressure and proteinuria levels. Previously, α7nAChR downregulation was ... See full document
9
α7 nicotinic acetylcholine receptor-mediated neuroprotection against dopaminergic neuron loss in an MPTP mouse model via inhibition of astrocyte activation
... Cells were collected and homogenized in 200 μl lysis buffer. After incubation for 20 minutes on ice, cell lysates were separated by centrifugation, and the protein concentration in the extracts was determined by ... See full document
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