[PDF] Top 20 Gene-expression analysis of a colorectal cancer-specific discriminatory transcript set on formalin-fixed, paraffin-embedded (FFPE) tissue samples

... 04897030001). Gene expression analysis was per- formed for eleven colorectal cancer specific markers [2] (CA7, COL12A1, CXCL1, CXCL2, CHI3L1, GREM1, IL1B, IL1RN, IL8, MMP3, ... See full document

... ovarian cancer, in particular with endometrioid ovarian ...from formalin-fixed paraffin-embedded (FFPE) samples leading to the identification of miRNAs that could be ... See full document

... Breast cancer remains a major public health problem in the western world, with a significant impact on mortality and ...breast cancer [1, ...Breast cancer is a heterogeneous disease and large numbers ... See full document

... from Formalin-Fixed Paraffin Embedded FFPE tissues is partially degraded, thus transcriptomic ana- lysis of FFPE tissues was considered challenging [13, ...transcriptomic ... See full document

... matic analysis, provisional mutation calls include numerous false positives and negatives ...during tissue handling and sequencing procedures, such as preparation of tissues into ... See full document

... from FFPE CNB and RP ...an FFPE optimized microarray containing probes targeting cancer specific content from multiple cancer ... See full document

... that cancer genome sequencing is likely to be integrated into routine clinical decision-making in the near future, many leading cancer research institutions and national cancer agencies have recently ... See full document

... to tissue slides containing primers at a operative solution concentration of ...were fixed in 4% formaldehydum polymerisatum, rinsed with distilled water, dehydrated in a graded series of alcohol and air ... See full document

... Briefly, FFPE tissues were obtained from the hysterec- tomy specimens. FFPE tissue blocks were sectioned on a standard microtome (Leica-microsystems RM 2145) to generate successive 10 μm ... See full document

... cytoplasmic expression of NF – κ B was seen in 85% of the ...cytoplasmic expression. Our study also showed nuclear expression of NF – κ B in 30% of ...more expression than differentiated ... See full document

... −203, −222-3p, −451, −490, −492, −509-5p, −571, −614, −622, and miR-93 [27]) which were significant at a 1% significance level, to account for multiple testing and with less than 10% missing values (indices II and V) and ... See full document

... 4-μm-thick FFPE tissue ...the Paraffin Pretreatment Kit (Vysis). Tissue sections were then digested with Di- gest All-3 (Zymed, San Francisco, CA, USA) twice for 5 ...min. Tissue ... See full document

... and embedded in paraffin as described previously ...two FFPE tonsil tissue blocks were sliced with a microtome and mounted onto glass ...adjacent tissue slices were stained with ... See full document

... 60 FFPE tissue sections used in this study were from the same SEER cases used in a previous study of whole exome sequencing (WES), with subject and specimen selection information described previously ... See full document

... the tissue microenvironment ...for tissue from multiple sections or slides to be collected into the same tube, minimizing potential for sample ...larger tissue sections or multiple tissue ... See full document

... method. Specific forward (5’-TCTTCCTCTGGGTATGGGTC-3’) and reverse (5’-ATTGGGTGTGCTGGATTCTC-3’) primers for LT Ag from previous authors works were used (17, ...the analysis, a duplicate test was performed ... See full document

... breast cancer patients who underwent excision of BM (Table ...HER2 expression, four primary tumor phenotypes were identified: hormone-receptor + and HER2– (23 cases), hormone-receptor + and HER2+ (21 ... See full document

... soft tissue tumors were collected from the Institute of Pathology of the Uni- versity Hospital in Heidelberg (Germany), in Kiel (Ger- many), in Jena (Germany), in Nijmegen and in Rotterdam (both the Netherlands), ... See full document

... UTUC is biologically distinct from UBUC. This is be- cause, in part, of the relative rarity of UTUC hindering large-scale molecular studies. Our study focused on FGFR3 aberrations in muscle-invasive UC and compared UTUC ... See full document

... 60 samples to the cobas BRAF V600 Mutation Test, a ...60 samples was expected to be adequate to demonstrate concordance of 95%–100% (kappa ...tumor samples (70%). Of these 60 FFPE tumor ... See full document