Top PDF HER 2 NEU and BMI 1 gene expression in Invasive Ductal Carcinoma Breast and Its correlation with hormone receptors and other known prognostic factors

HER 2 NEU and BMI 1 gene expression in Invasive Ductal Carcinoma Breast and Its correlation with hormone receptors and other known prognostic factors

HER 2 NEU and BMI 1 gene expression in Invasive Ductal Carcinoma Breast and Its correlation with hormone receptors and other known prognostic factors

Detailed history of the cases regarding age, sex, menstrual history, side of the breast, type of procedure, history of neo adjuvant therapy, details of gross characteristics such as tumor size, nodal status details were obtained for all the 238 mastectomy cases reported during the period from Surgical Pathology records. Freshly cut and Hematoxylin Eosin stained 4 µ thick sections of the paraffin tissue blocks of mastectomy specimens were reviewed and graded using the Nottingham modification of the Scarf Bloom Richardson Grading system (Annexure III) and they are further evaluated for the presence of necrosis, lymphocytic response and lymphovascular invasion by tumor. 20 cases of each grade from Invasive ductal carcinoma NOS subtype were randomly selected from the total cases and their representative formalin fixed paraffin embedded tissue samples were subjected to Immunohistochemistry for a panel of 4 markers and to real time reverse transcriptase polymerase chain reaction for gene analysis. The results were recorded with photographs. Follow up data of breast cancer patients regarding the adjuvant therapy, dose, duration, recurrence, disease free survival were obtained from Medical Records Section of Department of Oncology.
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Prognostic Implications of ER, PR and HER2/Neu Protein Expression in a Cohort of Breast Carcinoma

Prognostic Implications of ER, PR and HER2/Neu Protein Expression in a Cohort of Breast Carcinoma

Abstract: ER, PR and HER 2/neu receptor studies are known to be prognostic and predictive biomarkers of breast carcinoma. Hence the status of ER, PR and HER 2/neu receptors are routinely assessed in breast carcinoma by immunohistochemistry using paraffin embedded tissue blocks. The reason for assessing the receptor status is to decide on the best treatment regime for breast cancer patients. Ki 67 which is a biomarker of cellular proliferation is also assessed to guide the oncologist by determining the proliferative capacity of the tumour. A descriptive cross-sectional study conducted during January 2016 to December 2018 in three specialized surgical centres. Study sample included 92 patients with histologically confirmed breast carcinoma. ER, PR, HER2/neu receptor status and Ki 67 proliferative index were assessed to determine the prognostic implications of breast carcinoma. Mean age at presentation was 53.99 years and the most common histological type was invasive ductal carcinoma (84.78%). In the cohort of 92 patients with breast carcinoma 73.91% were ER positive, 58.69% were PR positive, and 11.95% were HER2/neu positive. Lymph nodal involvement was seen in 31.52% of the patients. There was no statistically significant association with HER2/neu status and nodal involvement (p = 0.629). Distant metastasis was seen in 4.35% cases. The association with HER2/neu status and distant metastasis was not statistically significant (p = 0.085). ER status showed a significant negative correlation with HER2 status (rho = -0.634, p < 0.0001). PR status showed a significant negative correlation with HER2 status (rho = -0.834, p < 0.0001). Ki67 index showed a significant positive correlation with HER2 status (rho = 0.248, p = 0.017).
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Correlation of Hormonal Receptors Estrogen Receptor, Progesterone Receptor and Her 2/Neu with Tumor Characteristics in Breast Carcinoma: Study of 100 Consecutive Cases

Correlation of Hormonal Receptors Estrogen Receptor, Progesterone Receptor and Her 2/Neu with Tumor Characteristics in Breast Carcinoma: Study of 100 Consecutive Cases

Breast cancer is the most common cancer of women in developed countries and 12% breast cancers occur be- tween 20 - 34 years [1]. In India, breast cancer is second to cancer of the cervix among women, but is consi- dered the leading cancer in certain metros such as Mumbai and Bangalore. It is estimated that approximately 80,000 cases occur annually. Breast cancer is strongly related to age, with only 5% of all breast cancers occur- ring in women under 40 years old [2]. Several pathological features have prognostic significance in breast car- cinoma which includes histologic subtypes, grade, lymph node status, estrogen receptor (ER)/progesterone re- ceptor (PR) status, growth factor and its receptors, proliferation activity and DNA content with tumor suppressor genes and oncogenes [3]. Prognosis of breast carcinoma is worse with higher grade, tumor subtypes like medul- lary and lobular carcinoma, lymph node metastasis, negativity for ER, PR and positivity for Her-e/neu, with excess of oncogenes and also presence of BRCA gene. Out of these, ER, PR and Her-2/neu immunostaining are a very useful tool for rapid diagnosis, treatment and prognosis of the patient. Determination of ER, PR status on biopsy specimens prior to therapeutic intervention is advocated as a standard practice [4]. Due to this, breast cancer is better represented by its combined receptor expression than by a single receptor status alone. Recent management protocol is focusing on separating triple negative and triple positive tumors for treatment and prognosis. With this background, the present study is undertaken to analyze the pattern of expression of hor- mone receptor ER, PR and Her-2/neu in invasive breast carcinoma and to correlate them with various clinicopa- thological parameters, especially in this part of country where studies are limited.
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Evaluation of HER-2/neu status in Gastric Carcinoma

Evaluation of HER-2/neu status in Gastric Carcinoma

A HER-2/neu:CEP17 (centromeric probe 17) ratio of >2.2 is now used to define HER-2/neu positivity (amplification). Ratios of 1.8–2.2 to define equivocal and <1.8 are used to denote negative categories. FISH is not routinely used for testing, because it is a difficult, cumbersome and expensive technique that requires trained personnel which is not available in every pathology laboratory. Moreover, fluorescence fades upon storage for a long time thus making it difficult to preserve the slides for further reference if needed. In addition, the fluorescent probes in the kits have a limited half life. Detailed morphological features of the tumor are also usually difficult to observe due to the required protein digestion and the fluorescent mode, and heterogeneity can be missed since spots are evaluated at ×100 magnification using oil immersion. (33)
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Differences in oestrogen and progesterone receptors, HER-2, p53 expression and proliferation in ductal breast cancers in relation to histopathological grade

Differences in oestrogen and progesterone receptors, HER-2, p53 expression and proliferation in ductal breast cancers in relation to histopathological grade

veillance. In 1999, under the auspices of the College of American Pathologists, a multi-disciplinary group of clinicians, pathologists and statisticians examined prognostic and predictive factors related to breast cancers and categorised them according to their value and the number of respective publications [4]. Three categories of prognostic factors were distin- guished. Factors of the first category carried an es- tablished clinical value and continue to be used in daily clinical practice (tumour size, metastases to the lymph nodes, grade, histological type, mitotic index and receptors for oestrogen and progesterone). The second category grouped factors whose significance required confirmation in subsequent clinical studies (HER-2, p53, Ki67, PCNA). Factors of the third cate- gory are at present at the stage of testing and their prognostic value has not yet been established (DNA ploidy, neoplastic angiogenesis, EGFR, TGF alpha, Bcl-2, pS2 and cathepsin D).
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Correlation of breast cancer risk factors with HER-2/neu protein overexpression according to menopausal and estrogen receptor status

Correlation of breast cancer risk factors with HER-2/neu protein overexpression according to menopausal and estrogen receptor status

Numerous epidemiological and experimental studies have shown the strong relationship between HER-2/neu- positivity and lack of hormone receptor expression in breast tumours [2,10,18,22]. In our study, HER-2/neu positive tumours were weakly related with the absence of estrogen receptors, although this was not statistically sig- nificant (see table 2). Because different ER status can result in different correlations between risk factors and HER-2/ neu+ breast cancer, it is always important to examine these interactions under ER stratification [8]. Since antiestro- gens can lower HER-2/neu levels in ER negative tumours, it is possible that an excess of estrogens can stimulate HER-2/neu in these tumours [8,18]. This mechanism could explain the stronger association between obesity (a situation with an overload of estrogens as mentioned above) and HER-2/neu-positivity among ER negative patients that was found in the present study (see table 5). The interview was conducted during the subjects' first visit to the unit and before clinical examination or any other intervention took place. This constitutes an advantage, because there was no chance that the subjects (both cases and controls) would be influenced by the diagnosis and might therefore falsely inflate the relative risk. Thus, the
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Prognostic value of ductal carcinoma in situ component in invasive ductal carcinoma of the breast: a Surveillance, Epidemiology, and End Results database analysis

Prognostic value of ductal carcinoma in situ component in invasive ductal carcinoma of the breast: a Surveillance, Epidemiology, and End Results database analysis

The following variables were extracted from the SEER database: age, race/ethnicity, tumor grade, tumor (T)-stage, nodal status, breast cancer subtype, radiotherapy, chemo- therapy, and surgical procedure, including breast-conserving surgery and mastectomy. The TNM classification was based on the seventh edition of the American Joint Committee on Cancer/Union for International Cancer Control breast cancer staging system. The T-stage was defined as the entire tumor size because the size of invasive component was not stated in the SEER program. Four major subtypes of breast cancer subtypes were defined as follows: hormone receptor (HoR) + /human epidermal growth factor receptor 2 (HER2)−, HoR + /HER2 + , HoR−/HER2 + , and HoR−/HER2. The HoR- positive disease was defined as estrogen receptor (ER) and/ or progesterone receptor (PR) positive. Patients were clas- sified into three groups in accordance with the DCIS com- ponent: No-DCIS, no DCIS component reported; L-DCIS, minimal DCIS component present ( < 25%); and H-DCIS, extensive DCIS component present ( ≥ 25%). The patterns of local and distant recurrence were not included in the SEER database. Therefore, we used breast cancer-specific survival (BCSS) as the primary survival outcome. BCSS was defined as the time from initial diagnosis to the date of breast cancer- related death.
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Bcl2 expression in breast carcinoma and its association with other clinicopathologic factors

Bcl2 expression in breast carcinoma and its association with other clinicopathologic factors

These data promise on a new approach to enhance the efficacy of endocrine treatment in estrogen receptor-positive (ER+) breast cancer by negating the antiapoptotic effect of BCL-2 using BH3 mimetics . Teixeira et al. (1995) reported that ER positive(MCF7 human) breast cancer cells had increased sensitivity to the cytotoxic agent doxorubicin when treated with antisense BCL-2(BH3 mimetics). Lastly, ER+ tumors were found to have a reduced likelihood of complete pathological eradication with chemotherapy or anti HER2 therapies than ER negative tumors. Hence Bcl2 antagonism in these ER+ tumors might increase the effectiveness of these predominately proapoptotic drugs when compared to the conventional treatment.
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To Study the Immunohistochemical expression of BRCA 1 in breast cancer and to assess its association with expression
of ER, PR and HER 2 receptors

To Study the Immunohistochemical expression of BRCA 1 in breast cancer and to assess its association with expression of ER, PR and HER 2 receptors

Its a rare type of breast carcinomas. It has low aggressive behaviour and is a counterpart of histologically similar tumor arising in salivary glands. Usually these tumors are bilateral and are situated around sub-periareolar region. Macroscopically they are well circumscribed lesion, cut surface of which appears pink or tan. Histopathologically three patterns can be made out (i) trabecular- tubular (ii) cribriform (iii) solid. Classically the cells are arranged in fenestrated nests or in tubules, cells can be either basaloid in nature with scant cytoplasm or cells with eosinophilic abundant cytoplasm with basaloid type of nucleus. It is a low grade malignant tumor and has a good prognosis, doesn’t spread via lymphatics.
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ROS1 Expression in Invasive Ductal Carcinoma of the Breast Related to Proliferation Activity

ROS1 Expression in Invasive Ductal Carcinoma of the Breast Related to Proliferation Activity

Most of the reported studies, previously cited, investi- gated aberrant changes in ROS1 genes or proteins in hu- man tumors, not the ROS1 gene or protein itself. There is no study in which the ROS1 gene and protein was exam- ined in breast cancer or in which its expression was com- pared with the clinicopathologic parameters or survival rate in any organ. To date, this is the first and only study to show that the ROS1 gene and ROS1 protein were ex- pressed in IDC and that these expressions were associated with several well-established prognostic factors. Several recent studies of lung cancer and cholangiocarcinoma have reported that a novel targeted therapeutic agent, such as multitargeted tyrosine kinase inhibitor, could be effective for the cases with ROS1 rearrangement or ROS1 fu- sion. 17,19,27,28 Further studies about aberrant genomic chang-
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Expression of Her-2/neu in extrahepatic cholangiocarcinoma

Expression of Her-2/neu in extrahepatic cholangiocarcinoma

Her-2/neu-positive breast carcinoma was used as a positive control. For negative control, the primary antibody was omitted during the incubation step. The EHBC-TMA was incubated for 16 min with a Her-2/neu rabbit monoclonal antibody (clone 4B5, using the Benchmark XT; Ventana Medical Systems) and detected by ultraview universal DAB detection kit (using the Benchmark XT; Ventana Medical Systems, Inc.). Antigen retrieval was performed using the Ventana Cell Conditioner #1 for 32 min. The Her-2/neu protein expression was evaluated using the modified ToGA trial scoring criteria established for gastric and gastroesophageal carcinomas 12–14 as follows: 1) no
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Association between Overexpression of Her 2 and Other Clinicopathologic Prognostic Factors in Breast Cancer in Morocco

Association between Overexpression of Her 2 and Other Clinicopathologic Prognostic Factors in Breast Cancer in Morocco

analysis. This pattern of overexpression of Her-2 in large tumors has been demonstrated in other studies [27,28] while in some studies this association has not been found [24,29-32]. The prognostic value of grade is currently established. Hoff et al. demonstrated in their series that high-grade tumors were more likely to have a positive HER-2 status as low-grade tumors [33]. Similarly, other studies have also reported the association between HER-2 positive status and a high histological grade [29-31]. This finding was also confirmed in our study. However, some studies have not demonstrated any cor- relation between high-grade status and HER-2 positive [24,34].The axillary lymph node involvement is a major prognostic factor. Most studies that have examined the prognostic role of HER-2 in N + patients showed that amplification of HER-2 gene or overexpression of Her-2/ neu protein is associated with pejorative results whether in univariate or multivariate analysis [35-37]. Our study found this association in univariate analysis, but disap- peared in multivariate analysis. Extra capsular spread and the vascular space invasion are two parameters that have been poorly studied in the literature and found to be as- sociated with overexpression of the protein Her-2/neu in our study. Despite the vast differences at the levels of the positivity of the Her-2, all the researchers report an in- verse correlation between HER-2 status and the hormone receptors [24,28-30,32,35,38-42]. Our data are consistent with those reported in the literature.
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Unique clinicopathological features of metaplastic breast carcinoma compared with invasive ductal carcinoma and poor prognostic indicators

Unique clinicopathological features of metaplastic breast carcinoma compared with invasive ductal carcinoma and poor prognostic indicators

At initial diagnosis, all tumor specimens were reviewed by two pathologists at the 3rd AHHMU. Tumor specimens were histologically examined, and cases were included in the re-review (55 MBC cases) if adenocarcinomatous elements were identified as admixed with SCC, spindle cell, chondroid or osseus tumor basing on hematoxylin and eosin staining and/or immunohistochemistry (IHC). Four-micron tissue sections, prepared from a formalin-fixed paraffin-embedded representative of the tumor samples, were used. The histological grade was determined according to the modified Bloom-Richardson classification. IHC staining for ER and PR (Zhongshan-Bio Co., Beijing, China) was performed using a conventional detection method and ER and PR status was evaluated based on the percentage of positively stained nuclei [13]. Positive staining for HER2 (Zhongshan-Bio Co.) was defined based on the percentage of tumor cells and the intensity of membrane staining. HER2 was scored as 0 to 3+ according to the method recommended for the DakoHercepTest. Tumors with scores of 3 or with a >2.2- fold increase in HER2 gene amplification as determined by fluorescence in situ hybridization were considered to be positive for HER2 over-expression [14]. Cells stained for Ki-67 and P53 were counted and expressed as a percentage. Low expression was considered as Ki-67<14% [15] and P53<25% [5]. All protocols were reviewed and approved by the Ethical Committee of Harbin Medical University in Harbin, China. Informed consent was obtained from all the patients.
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Prognostic significance of RSPO1, WNT1, P16, WT1, and SDC1 expressions in invasive ductal carcinoma of the breast

Prognostic significance of RSPO1, WNT1, P16, WT1, and SDC1 expressions in invasive ductal carcinoma of the breast

This study included tumor tissues surgically resected from 100 patients who visited Uijeongbu Mary’s Hospital and were diagnosed with invasive ductal carcinoma of the breast between 2002 and 2004. Patients’ age ranged between 29 and 77 (mean, 49.5) years old. All patients were diagnosed as invasive ductal carcinoma; 43 cases received adjuvant chemotherapy, 35 cases received hormone therapy, and 21 cases received adjuvant radiotherapy. Disease-free survival data (median, 62.8 months; range, 12.8 to 103.3 months) was available. The disease relapsed in 19 patients and 6 patients died of the disease. Using the tissues, tissue microarray blocks were constructed and used for immunohistochemical staining. Human tissue acquisition and its use followed the Institutional Review Board-approved protocol (CUMC11U058) from the Cath- olic University of Korea, School of Medicine.
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The impact of nottinghem prognostic index (NPI) on the occurrence of relapses in her 2 / NEU positive breast cancer

The impact of nottinghem prognostic index (NPI) on the occurrence of relapses in her 2 / NEU positive breast cancer

sections with a thickness of 2.5 microns, were placed in special pretreated slides (Poly-l-lysine) and dried overnight at a temperature of 60 0 С. They were dewaxed in xylene and dehydrated in alcohol with different concentrations (100%, 96%, 80%). Epitope retrieval of HER2 / neu receptor was used in water bath at a temperature of 96 0 С, and forimmunohistochemical studies of proliferative index Ki67, and protein products of the p53 tumor suppressor gene citrate buffer was used in a microwave oven of 700W, according to the requirements of the manufacturer. Imunohistohemical analysis was performed by avidin-biotin complex technique. Evaluation of HER-2 / neu gene was performed by ready-to- use, HercepTest kit (DAKO, Glostrup, Denmark), and the for the immunohistochemical analysis of Ki67 proliferative index and the protein product of the tumor suppressor gene p53 prepared monoclonal murine antibody (DAKO, Glostrup, Denmark) and visualizing system (Dako REAL ™ EnVision ™ Detection System, Peroxidase / DAB +, Rabbit / Mouse) have been used with the dilution of 1:50. At the slides with a HER-2 / neu positive and negative controls were added for accurate evaluation and control staining. After the performed steps of immunohistochemical staining, slides were counterstained with hematoxylin. Evaluation of HER-2 / neu consists in detecting strong membrane staining in at least 10% of malignant cells in the invasive front of the tumor, which was considered as positive result and indicated with 3+ (Wolff et al., 2007). Evaluation of the nuclear signal for Ki67 proliferative index and the protein product of the tumor suppressor gene p53 was performed according to the rules of Bhargava (2009) and Reed (2000) by counting at least 10 high power fields (HPF, x40) which were considered as positive on 14% of nuclei of malignant cells for Ki67 and over 10% of nuclei of cancer cells for p53. The type of the antibody, clone, dilution, the manufacturer and the cut-off point of a positive signal for the tested antibodies are shown in Table 1.
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Association of tumour necrosis factor alpha and its receptors with thymidine phosphorylase expression in invasive breast carcinoma

Association of tumour necrosis factor alpha and its receptors with thymidine phosphorylase expression in invasive breast carcinoma

In light of previous studies showing TAMs to be the only major source of TNF-a in breast carcinoma Miles et al, 1994; Pusztai et al, 1994; Lewis and McGee, 1996, we have interpreted our [r]

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Hormone receptor expression and survival patterns in operated cases of female invasive ductal breast carcinoma in Kerala: a retrospective cohort study

Hormone receptor expression and survival patterns in operated cases of female invasive ductal breast carcinoma in Kerala: a retrospective cohort study

4% of all cases reported in literature [25]. The study co- hort has a similar fraction of patients (5.3%) with ER − PR+ expression, and 90% survival has been noted in this particular group. The predictive value of PR in the ab- sence of ER expression is controversial [26,27]. Some re- ports suggest that positive PR in the absence of ER has a higher response to hormone therapy, but this finding is not universal [28-30]. Unfortunately, because of the rela- tively small number of patients in the study group who belonged to this subset, it is difficult to comment on the role of this particular type of receptor expression on dis- ease outcome.
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Expressions of FHIT, BCRP and Bcl-2 Proteins in Breast Invasive Ductal Carcinoma

Expressions of FHIT, BCRP and Bcl-2 Proteins in Breast Invasive Ductal Carcinoma

Numerous factors influence the occurrence and development of tumor cells with sophisticated interaction mechanisms. Studies have found that BCRP and Bcl-2 proteins may be engaged in the interaction between cells and between cells and substrate through adjusting the cell membrane receptor and the cellular signal transduction pathway. This will loosen the connection between cells and make the intercellular signal transmission abnormal, which down-regulates the expression of FHIT protein, thus preventing the apoptosis of the infiltrating ductal cancer cells and promoting the occurrence and development of invasive ductal carcinoma [19]. The results of this study show that in breast IDC, the expression of FHIT protein was negatively correlative with those of BCRP and Bcl-2, but the expression of BCRP protein was positively correlative with that of Bcl-2, indicating that they independently and cooperatively participated in the occurrence and development of breast IDC. In addition, the measurement and evaluation of ER, PR and Her-2 in breast cancer has been widely used in clinic. The expressions of ER and PR provide theoretical basis for the postoperative endocrine therapy. Her-2 also has become an important indicator in predicting tumor prognosis. However, this study does not find that there is a significant correlation between the expressions of FHIT, BCRP and Bcl-2 and those of ER, PR and HER-2 (Table 2), which is consistent with previous researches, surmising that the occurrence and development of breast IDC results from multiple factors. As each factor has phenotypic advantage and signal-regulated network, FHIT, BCRP and Bcl-2 in the breast IDC generation and development may not be directly associated with ER, PR and HER-2, and the specific mechanism cannot be explained clearly by genetic expression in a stationary phase, and needs to be further explored by functional morphology.
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A study on role of HER 2/NEU over expression in gastric carcinoma in south Indian population

A study on role of HER 2/NEU over expression in gastric carcinoma in south Indian population

Sex difference with male preponderance is correlating with others studies owing to risk factors such as smoking and drinkind are common among males. Gastric adeno carcinoma even thogh it is a disease of the 6 th and seventh decade , occurred at even young age of 20 years. A.Ieni and his colleagues studied the incidence of her2/neu in gastric carcinoma patients. The mean age in their study 68.3 years and the youngest incidence was 41 years{A.IENI, 2013 #2}. But our study reveals the mean age to be a decade less than Sicilian study. Antral carcinomas are common which could be explained by association with H.pylori infection.
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Study of the Expression of miR-4270 in Plasma of Patients with Breast Invasive Ductal Carcinoma

Study of the Expression of miR-4270 in Plasma of Patients with Breast Invasive Ductal Carcinoma

Forty blood samples were collected from women with invasive ductal breast cancer who were referred to Noor-Nejat Tabriz hospital from 2015 to 2017. The breast cancer patients were aged between 27 and 80 years (mean age of 51.8 years) and had undergone breast surgery. All blood samplings were performed in the pre- operative stage. Twenty-eight blood specimens were also taken from healthy women volunteers who had no history of cancer in their first-degree relatives. The research ethics committee of Noor-Nejat hospital approved the study in accordance with the institutional protocol and informed consent was obtained from all patients. The plasma was isolated from blood specimens and maintained at -80 ºC temperature. The clinical and pathological information of the patients is shown in table 1.
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