[PDF] Top 20 Expression of polyomavirus large T antigen by using a baculovirus vector.
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Expression of polyomavirus large T antigen by using a baculovirus vector.
... An intronless construct of the PyTAg gene was obtained from plasmid pspLT5 28 by BamHI and XhoI digestion, purified by gel electrophoresis, and inserted into KpnI- and XhoI-digested pEV-[r] ... See full document
5
J Domain-Independent Regulation of the Rb Family by Polyomavirus Large T Antigen
... (A) Expression of wild-type LT induced loss of C2C12 ...control vector (CON) or wild-type (WT) ...control vector (CON) or wild-type LT in the presence or absence of 1 g of Bcl2 and treated as ... See full document
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Merkel Cell Polyomavirus Large T Antigen Has Growth-Promoting and Inhibitory Activities
... MCPyV large T antigen Exon 3, green fluorescent protein (GFP), or empty ...control vector in BJ-hTERT cells that stably expressed H-Ras and SV40 large and small T antigens ... See full document
9
Polyomavirus Large T Antigen Binds Symmetrical Repeats at the Viral Origin in an Asymmetrical Manner
... NG59RA polyomavirus origin-containing plasmid pFLORI40 or their respective mutants P1, P2, P3, or P4, together with LT, for 48 ...amplified using PCR analysis with specific primers and visualized by ... See full document
9
Polyomavirus T Antigen Induces APOBEC3B Expression Using an LXCXE Dependent and TP53 Independent Mechanism
... types. Polyomavirus and papillomavirus oncoproteins induce APOBEC3B overexpression, perhaps to their own benefit, but little is known about the cellular mechanisms hijacked by these viruses to do ...the ... See full document
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Polyomavirus Large T Antigen Induces Alterations in Cytoplasmic Signalling Pathways Involving Shc Activation
... the expression of receptors, and (iii) the activities of kinases or phosphatases involved in the control of Shc phosphorylation, as well as, of course, a simultaneous alteration of the above-mentioned ... See full document
11
Polyomavirus Large T Antigen Binds the Transcriptional Coactivator Protein p300
... By using the calcium phosphate precipitation method, a GAL4-p300 construct (kindly provided by Antonio Giordano) was transfected into NIH 3T3 cells together with a promoter- CAT construct carrying GAL4 binding ... See full document
6
The Minimum Replication Origin of Merkel Cell Polyomavirus Has a Unique Large T-Antigen Loading Architecture and Requires Small T-Antigen Expression for Optimal Replication
... wild-type T-antigen genomic expression construct ...genomic T antigen cassette as TAg, which expresses sT and 57kT proteins from alterna- tively spliced mRNAs in addition to LT protein ... See full document
11
Elements of the polyomavirus replication origin required for homologous recombination mediated by large T antigen.
... SNV-derived vector (p k B4) bearing two copies of an oligonucleotide with two NF- k B mo- tifs at the late border of ori-core ...with polyomavirus large T-ag with and without various Rel/ k B ... See full document
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Zinc-binding and protein-protein interactions mediated by the polyomavirus large T antigen zinc finger.
... entire polyomavirus origin used as a probe. Baculovirus-expressed LT was used to bind DNA con- sisting of an EcoRI-DdeI double digest of ...the polyomavirus origin ...the polyomavirus origin ... See full document
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Cyclin-dependent kinase regulation of the replication functions of polyomavirus large T antigen.
... Py large T Ag lacking sequences between bp 962 and 1338 bp and there- fore lacking T187 was both capable of initiating DNA replica- tion and able to complete a viral infection cycle in rapidly growing mouse ... See full document
7
Tiny T antigen: an autonomous polyomavirus T antigen amino-terminal domain.
... tiny T antigen protein. Previous analyses of the expression of muPy T antigens in infected or transformed cells did not detect tiny T antigen, probably because the electro- ... See full document
7
Altered phosphorylation pattern of simian virus 40 T antigen expressed in insect cells by using a baculovirus vector.
... 4799 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The phosphorylation pattern of simian virus 40 SV40 large tumor T antigen purified from insect cells infected with [r] ... See full document
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Abundant expression of polyomavirus middle T antigen and dihydrofolate reductase in an adenovirus recombinant.
... Two cDNA segments have been inserted into this adenovirus vector and expressed at high levels: one encoding the pymT Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest -p [r] ... See full document
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Novel Human Polyomavirus Noncoding Control Regions Differ in Bidirectional Gene Expression according to Host Cell, Large T-Antigen Expression, and Clinically Occurring Rearrangements
... viral expression profile is intriguing and suggests a first functional characterization of secondary HPyV host cell tropism through this bidirec- tional reporter ...reporter vector should be amenable to ... See full document
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The Retinoblastoma Protein Alters the Phosphorylation State of Polyomavirus Large T Antigen in Murine Cell Extracts and Inhibits Polyomavirus Origin DNA Replication
... Py T Ag, human pRb, or human ...Py T Ag (84) or p53 (82) was immunopurified on PAb F5 or PAb 421 columns, respectively, as previously ...pRb baculovirus generously provided by ... See full document
10
Activation of CREB/ATF Sites by Polyomavirus Large T Antigen
... of polyomavirus large T) (33), pCMV P43S LT (which contains a mutation in the HPD loop of the J domain) (67), pCMV NT (residues 1 to 259) (33), and pCMV-CT (residues 264 to 785) (27) have been ... See full document
11
Transgenic Expression of Polyomavirus Middle T Antigen in the Mouse Prostate Gives Rise to Carcinoma
... the expression of an MT transgene to only those tissues in which cre was expressed via either a second transgene or a viral vector ...recombinase expression (Probasin-Cre) in the prostate, MT ... See full document
12
Constitutive expression of simian virus 40 large T antigen in monkey cells activates their capacity to support polyomavirus replication.
... Similar results were observed with three different DNAs containing wild-type polyomavirus origins, and we have observed replication of polyomavirus DNAs in two other types of monkey cell[r] ... See full document
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Phosphorylation of polyomavirus large T antigen: effects of viral mutations and cell growth state.
... Early in infection of quiescent cells intermediate mobility forms of large T antigen with little or no phosphorylation, particularly of peptides 5 and 7, were seen, whereas peptides 5 an[r] ... See full document
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