[PDF] Top 20 Gene Expression Profile of Adherent Cells Derived From Human Peripheral Blood: Evidence of Mesenchymal Stem Cells

... dayof culture. Five micrograms of total RNA isolated from PB-MSCs was subjected to DNase I treatment (Fermentas, Thermo Scientific, Waltham, MA, USA) to remove any DNA contamination. The electrophoresis on a 1% ... See full document

... aspirated from the posterior iliac crest after induction of ...mononuclear cells (MNCs) using Ficoll-paque density gradient separation method and the other mature blood cell lineages were ... See full document

... that human bone marrow-derived MSCs (BMSCs) are capable of transdiffer- entiating into hepatocytes and repopulating the liver in vivo through albumin secretion and the gene expression ... See full document

... the cells encounter a second- round ...the expression of B cell-activating factor (BAFF) could be increased in re- sponse to TLR4 stimulation ...Exosomes from TLR4-primed UC-MSCs are known to possess ... See full document

... transgene expression may be a contributory ...somatic cells in a manner suitable for hiPSC ...trans- gene expression and also possesses an ... See full document

... Mesenchymal stem cells (MSCs) derived from human umbilical cord (UC) have been considered as an important tool for treating various malignancies, tissue repair and organ ... See full document

... Until now, several clinical trials have used MSCs to treat autoimmune diseases, such as diabetes, rheumatoid arthritis, Crohn's disease, lupus erythematosus and multiple sclerosis, and some of these studies have found ... See full document

... its expression in hMSCs but 150kDa HA decreased it in ...(started from day 21). Stem cell differentiation direction and degree were effected by many factors in the environment, such as cell ... See full document

... up from monolayer culture as it has a geometry and substrate similar to those of a ...6 cells), four to 10 four-layered cell factors were ...of cells and in harvest- ing could result in increased ... See full document

... ranging from approximately 40 nm to a few mm in size, into their extracellular ...between cells, organs and even between organisms, and have been detected in body fluids, such as blood, urine, ... See full document

... MSCs may also protect against S. mansoni-induced liver fibrosis by altering the microenvironment of the liver at sites of engraftment. This microenvironment is supposed to be improved in presence of PZQ, since it was ... See full document

... the expression of overlapping sets of transcription factor genes including Twist1, Sox9, Msx1, and Msx2 ...adipose-derived human mesenchymal stem cells (Ad-MSCs) using a ... See full document

... purchased from Orient Biosciences (Seoul, Republic of Korea) were used in this ...of stem cells was evaluated using an optimized GalN/LPS dose (single injection; 1500 mg/kg GalN and 20 μg/kg ... See full document

... Animal model fabrication of deep second-degree burn Animal care and experiments were performed in accord- ance with the protocols approved by the Animal Care and Use Committee of Shandong University School of Basic ... See full document

... As previously mentioned, despite the well-known advan- tageous biological properties of MSCs, they have not been successfully adapted in clinical trials, because of the lack of standardized protocols, and this has ... See full document

... neoplastic cells persist and inevitably give rise to recurrent ...arising from the growing tumor, and the deleterious effects of surgery and adjuvant therapies ...needed. Stem cells may ... See full document

... PBS). The sucrose solution was removed and the EBs were placed on the mold. OCT was slowly added to the mold to facilitate the assembling of cell aggregates at the center of the mold. The mounted cell cluster–OCT blocks ... See full document

... Schwann cells (SCs) has shown a great promising result for SCI but harvesting SC is limited due to donor complications and limited cell collection ...of stem cells to differentiate into SCs can ... See full document

... The cells were transplanted 3 weeks after percutaneous coronary intervention (PCI), which itself occurred 8 days after ...3 cells if cultured for 14 –21 days, depending on the starting ...cardiac ... See full document

... Menstrual blood-derived MSCs are attractive because they are easy to collect (compared to bone marrow as- piration), have longevity in culture, and can be subcul- tured to 10–20 passages with retention of a ... See full document