[PDF] Top 20 miRNA expression profiling in formalin-fixed paraffin-embedded endometriosis and ovarian cancer samples
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miRNA expression profiling in formalin-fixed paraffin-embedded endometriosis and ovarian cancer samples
... in endometriosis versus normal tissue and ovarian cancer versus normal tissue, and we were also able to identify a panel of miRNAs that are differentially expressed in ovarian cancer ... See full document
14
Molecular differential diagnosis of follicular thyroid carcinoma and adenoma based on gene expression profiling by using formalin-fixed paraffin-embedded tissues
... misclassified samples the same that would be misclassified by histopathologists? The excellent prognosis of follicular cancer treated with adequate surgical and adjuvant therapy makes this assessment ... See full document
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Original Article MicroRNA Expression Profiling Outperforms mRNA Expression Profiling in Formalin-fixed Paraffin-embedded Tissues
... analysis, miRNA expression profiles from fresh frozen and FFPE tissue sources cluster most closely according to the patient from which they were ...mRNA expression profiles are most similar among the ... See full document
9
Expression profiling with RNA from formalin-fixed, paraffin-embedded material
... breast cancer spec- imen which resulted in intact RNA in all samples ...Gene expression was measured by QPCR using 25 commercially available and three own TaqMan assays [13] ...the expression ... See full document
15
Expression profiling of formalin-fixed paraffin-embedded primary breast tumors using cancer-specific and whole genome gene panels on the DASL®platform
... (UHRR) samples (Agilent Technologies, Santa Clara, CA) comprised from 10 human cell lines were used as control RNA samples to assess the quality of RNA labeling and hybridization in each DASL ...all ... See full document
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Analytical validation of a prognostic prostate cancer gene expression assay using formalin fixed paraffin embedded tissue
... 40) samples followed by microarray genomic profiling on both ...of samples (10 CNB, 10 RP) were processed in duplicate giving a total sample number of 75 samples profiled on each ...gene ... See full document
11
Indoleamine 2,3-dioxygenase expression and overall survival in patients diagnosed with breast cancer in Pakistan
... tumors were T1 and 89.1% tumors were T2/T3. The grade distribution was I, II and III respectively. 61.5% tumors were presenting grade III. The patients’ positive for node and metastasis were 57.5% and 56.3%, ... See full document
7
Prognostic Significance of Promoter Hypermethylation and Diminished Gene Expression of SYNPO2 in Melanoma.
... Gene expression signatures have been defined that are associated with metastatic capacity of primary melanoma and survival (Winnepenninckx et ...deregulated expression of genes involved in ... See full document
15
Recombinant "IMS TAG" proteins : a new method for validating bottom up matrix assisted laser desorption/ionisation ion mobility separation mass spectrometry imaging
... Mass spectrometry images of the distribution of proteins in fresh frozen and formalin fixed paraffin embedded tissue samples following in situ tryptic digestion were generated by isolati[r] ... See full document
28
Identification of germ cell-specific VASA and IFITM3 proteins in human ovarian endometriosis
... Paraffin sections were dewaxed in xylene and hydrated through decreasing ethanol. After 10 min in PBS, the heat- induced epitope retrieval was performed by thermal bath at 96 °C in 10 mM sodium citrate at pH 6 ... See full document
7
Expression of P 16 ink4a in basal like triple negative breast carcinoma in Sudanese women
... like cancer and raise the possibility that triple – negative breast cancer with cytokertin, and p16 co-expression may adequate identify these tumours and serve as potential diagnostic / prognostic ... See full document
12
PTPRO promoter methylation is predictive of poorer outcome for HER2 positive breast cancer: indication for personalized therapy
... A total of 221 breast tissue specimens were harvested and preserved from patients between June 1998 and December 2010 at the Affiliated Cancer Hospital of Shantou University Medical College. The median age of the ... See full document
8
Integrating precision cancer medicine into healthcare—policy, practice, and research challenges
... in Cancer (COSMIC) [62], the cBioPortal for Cancer Genomics [63], the Therapeutically Applicable Research to Generate Effective Treatments (TARGET) [64], the Pediatric Cancer Genome Project [65], or ... See full document
12
Detection of Survivin Gene Expression in Formalin-Fixed Paraffin-Embedded Tissues of Human Osteosarcoma: Its Potential Usefulness in Diagnosis and Prognosis of Bone Tumors
... between expression of survivin and unfavorable outcome of cancer, it may be used as a specific molecular marker for prognosis and classification of malignant tumors ... See full document
7
Sensitive and reliable detection of Kit point mutation Asp 816 to Val in pathological material
... In daily routine the amount of available material as well as the handling (e.g. fixation) varies. FFPE is the most widely used material in pathological institutes with conse- quences for the nucleic acids. During the ... See full document
5
Evaluation of PCR in Detection of Mycobacterium tuberculosis from Formalin Fixed, Paraffin Embedded Tissues: Comparison of Four Amplification Assays
... between samples included frequent changing of gloves, minimal handling of samples, preparation of complete reaction mix- tures before adding template DNA, and capping of all tubes immediately before ... See full document
6
Overexpression of c-erb B2 is a negative prognostic factor in anaplastic astrocytomas
... The overexpression of EGFR in anaplastic astrocyto- mas is in line with our earlier report and other studies [24,28,32-34]. Interestingly, the antibody reactive against the cytoplasmic domain unveiled far more ... See full document
9
Diagnostic Examination of Human Intestinal Spirochetosis by Fluorescent In Situ Hybridization forBrachyspira aalborgi, Brachyspira pilosicoli, and Other Species of the Genus Brachyspira(Serpulina)
... Human intestinal spirochetosis (HIS) is a condition of the large intestinal mucosa microscopically characterized by colo- nization and extensive end-on attachment of densely packed spirochetes to the epithelial surface ... See full document
8
Next-generation sequencing to guide cancer therapy
... locations. Cancer centers that have created and host these databases include MD Anderson’ s Per- sonalized Cancer Therapy [42, 43], Vanderbilt’s My Can- cer Genome [44, 45], and the Broad Institute’s TARGET ... See full document
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Molecular characterization of high risk human papillomavirus genotypes involved in invasive cervical cancer from formalin fixed, paraffin embedded tissues in Ouagadougou, Burkina faso
... cervical cancer; they are preceded by other genotypes (de Sanjose et ...cervical cancer confirmed ...cell samples from women with unknown pathology status and/or HIV (human immunodeficiency virus) ... See full document
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