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[PDF] Top 20 Expression of a recombinant bacterial l asparaginase in human cells

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Expression of a recombinant bacterial l asparaginase in human cells

Expression of a recombinant bacterial l asparaginase in human cells

... The human granulocyte colony stimulating factor produced in mammalian cells was not recognized by patient anti- bodies, while the same biopharmaceutical produced in bacteria and yeast was reactive [18, ... See full document

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Expression, purification and evaluation of recombinant L-asparaginase inmehthylotrophic yeast Pichia pastoris: Expression, purification and evaluation of recombinant L-asparaginase in mehthylotrophic yeast Pichia pastoris: Research article

Expression, purification and evaluation of recombinant L-asparaginase inmehthylotrophic yeast Pichia pastoris: Expression, purification and evaluation of recombinant L-asparaginase in mehthylotrophic yeast Pichia pastoris: Research article

... of recombinant vector corre- sponds to lane 1-21 and lane 23-24 indicating the inser- tion of asn gene ...pastoris cells, the recombinant vector was linear (4581 bp) for integration into yeast genome ... See full document

5

Optimization of recombinant human transferrin expression in insect cells baculovirus system

Optimization of recombinant human transferrin expression in insect cells baculovirus system

... manufacturers. It is expected to increase to 3.69 million liters in 2011 at a compound annual growth rate (CAGR) of 7.2 per cent. A variety of systems can be employed to produce biopharmaceuticals. The most important ... See full document

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Media optimization and growth parameters for the production of L asparaginase enzyme from bacterial isolate

Media optimization and growth parameters for the production of L asparaginase enzyme from bacterial isolate

... INTRODUCTION L-asparaginase is therapeutic enzyme acting on L-asparagine and a promising product for the treatment of Acute Lymphocytic ...of L-asparagine by asparaginase catalytic ... See full document

6

Signal peptides for recombinant protein secretion in bacterial expression systems

Signal peptides for recombinant protein secretion in bacterial expression systems

... the recombinant B. subtilis cells varied significantly depending on the nature of the signal peptide that was used to direct the cutinase into the Sec export ... See full document

10

Expression and characterization of the human erythrocyte glucose transporter in insect cells using a recombinant baculovirus

Expression and characterization of the human erythrocyte glucose transporter in insect cells using a recombinant baculovirus

... the cells blotted onto a ...putative recombinant-containing agarose plugs are picked and subjected to further screening and ...the cells tend to smear during blotting, enlarging the apparent diameter ... See full document

325

Cloning and Expression of Recombinant Human Interleukin-7 in Chinese Hamster Ovary (CHO) Cells

Cloning and Expression of Recombinant Human Interleukin-7 in Chinese Hamster Ovary (CHO) Cells

... CHO cells to perform post- translational modifications, such as glycosylation, has made them as an attractive expression system for the manufacture of therapeutic ...CHO cells can secrete large ... See full document

8

Expression of Recombinant Human Glucocerebrosidase Protein in Sunflowers

Expression of Recombinant Human Glucocerebrosidase Protein in Sunflowers

... A bacterial suspension was used to transform sunflower ...16h L/8h ...mRNA expression in modified sunflowers was detected by qRT-PCR compared with control GBA ... See full document

16

Expression of Biologically Active Recombinant B-Domain-Deleted Human Factor VIII in Mammalian Cells

Expression of Biologically Active Recombinant B-Domain-Deleted Human Factor VIII in Mammalian Cells

... either human plasma derived FVIII or recombinant ...a recombinant form of biologically active human coagulation factor VIII, a mammalian expression system is necessary for proper ... See full document

10

Expression of innate immune complement regulators on brain epithelial cells during human bacterial meningitis

Expression of innate immune complement regulators on brain epithelial cells during human bacterial meningitis

... to bacterial infection including the expression of tumour necrosis fac- tor-α [31,32] and ICAM-1 to facilitate neutrophil invasion ...thelial cells remained largely ...epithelial cells to ... See full document

9

Evaluation of expression systems of recombinant human interferon gamma

Evaluation of expression systems of recombinant human interferon gamma

... improve expression/ secretion of recombinant hIFNγ in ...low expression/ secretion might be intracellular degradation of hIFNγ in ...that expression of modified versions of bovine and chicken ... See full document

145

Transient, nonlethal expression of genes in vertebrate cells by recombinant entomopoxviruses.

Transient, nonlethal expression of genes in vertebrate cells by recombinant entomopoxviruses.

... b-Galactosidase expression of AmEPV esp-lacZ in different verte- brate cell ...5 cells/well in a 12-well plate, seeded 1 day before infection and infected with AmEPV esp-lacZ recombinant virus at an ... See full document

6

Optimization of fermentation medium to maximize the production of recombinant human asparaginase in Escherichia coli through the statistical design of experiments

Optimization of fermentation medium to maximize the production of recombinant human asparaginase in Escherichia coli through the statistical design of experiments

... Selection of Carbon and Nitrogen Sources Carbon and nitrogen sources are the crucial primary nutrients for any kind of bacterial fermentation. Numerous experiments were carried out to select the best carbon and ... See full document

11

Differential gene expression in human granulosa cells from recombinant FSH versus human menopausal gonadotropin ovarian stimulation protocols

Differential gene expression in human granulosa cells from recombinant FSH versus human menopausal gonadotropin ovarian stimulation protocols

... Moreover, genes not previously studied in the ovary may reveal important new regulatory mechanisms. Conclusions GC recovered from IVF patients at oocyte retrieval who were stimulated with either rFSH or hMG displayed ... See full document

6

Expression and extracellular release of human immunodeficiency virus type 1 Gag precursors by recombinant baculovirus-infected cells.

Expression and extracellular release of human immunodeficiency virus type 1 Gag precursors by recombinant baculovirus-infected cells.

... The level of synthesis and extracellular release of human immunodeficiency virus type 1 Gag by insect cells was analyzed, using eight different recombinants of Autographa californica nuc[r] ... See full document

6

Increased transgene expression mediated by recombinant adeno-associated virus in human neuroglia cells under microgravity conditions

Increased transgene expression mediated by recombinant adeno-associated virus in human neuroglia cells under microgravity conditions

... The expression level of the accessory receptors affects the endocytosis of rAAV2 entering ...the expression of rAAV2 and virus receptors was detected at 24h, 48h and ...that cells couldn’t adapt to ... See full document

11

Expression and characterization of recombinant human NEIL3 from Escherichia coli

Expression and characterization of recombinant human NEIL3 from Escherichia coli

... occurring human mutation in the N-terminal DNA glycosylase domain of NEIL3 was ...B cells from the spleen and also germinal centre B cells in comparison to wildtype ... See full document

158

Expression of the functional recombinant human glycosyltransferase GalNAcT2 in Escherichia coli

Expression of the functional recombinant human glycosyltransferase GalNAcT2 in Escherichia coli

... the bacterial oligosaccharyltransferase PglB from ...various bacterial strains such as Neisseria and Acinetobacter [14,15] further emphasises the future potential of glycoengineered bacteria as cell ... See full document

12

Bacterial based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance

Bacterial based systems for expression and purification of recombinant Lassa virus proteins of immunological relevance

... improved expression of both LASV glycoproteins, allow- ing us to achieve the highest yield of recombinant protein per gram of cell mass in an environment appropriate for generation of conformationally ... See full document

14

Construction of a recombinant eukaryotic expression vector containing PHD3 gene and its expression in HepG2 cells

Construction of a recombinant eukaryotic expression vector containing PHD3 gene and its expression in HepG2 cells

... PHD3 expression was significantly increased after therapy with epirubicin, alone or in combination with tamoxifen, in patients with T2-4 N0-1 breast cancer; however, PHD3 expression was not relevant in ... See full document

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