[PDF] Top 20 Fluorescence resonance energy transfer (FRET) as a method to calculate the dimerization strength of basic Helix-Loop-Helix (bHLH) proteins
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Fluorescence resonance energy transfer (FRET) as a method to calculate the dimerization strength of basic Helix-Loop-Helix (bHLH) proteins
... acceptor fluorescence. The fluorescence emission from the donor and the acceptor are collected sequentially using the “multitrack” function of the Zeiss LSM 510 ...Average fluorescence intensities of ... See full document
5
Use of Fluorescence Resonance Energy Transfer Hybridization Probes To Evaluate Quantitative Real Time PCR for Diagnosis of Ocular Toxoplasmosis
... double fluorescence resonance energy transfer hybrid- ization probe system with a double fluorescence reading for each sample (one for the LC-Red 640 used for ... See full document
5
Modern fluorescent techniques to investigate the mechanisms of lymphocyte activation
... for fluorescence microscopy were designed specifically for synthetic ...in fluorescence microscopy or other biological techniques: resistan- ce to photodestruction, brightness, the number of colors ... See full document
13
Homomultimerization of the Coxsackievirus 2B Protein in Living Cells Visualized by Fluorescence Resonance Energy Transfer Microscopy
... fusion proteins in vivo, we investigated the occurrence of FRET by ...fusion proteins leads to the quenching of the ECFP donor and sensitized emission of the EYFP ...SPIM, fluorescence emission ... See full document
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Bioluminescence Resonance Energy Transfer System for Measuring Dynamic Protein Protein Interactions in Bacteria
... regulatory proteins function by binding to promoters of their target genes as multimers upon receiving proper environmental ...testing proteins X and Y do not interact, eYFP cannot be excited by the ... See full document
10
Nucleosome dynamics resolved with single-pair fluorescence resonance energy transfer spectroscopy
... histone proteins are known to stick to many types of glass and plastic surfaces ...single-molecule fluorescence microscopy several immobilization schemes have been employed successfully (reviewed by Rasnik ... See full document
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In Vivo Self-Interaction of Nodavirus RNA Replicase Protein A Revealed by Fluorescence Resonance Energy Transfer
... of fluorescence resonance energy transfer (FRET), spectrofluorometric analysis of bioluminescence resonance energy transfer, and coimmunoprecipita- ... See full document
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EFFECT OF HYDROGEN BONDING ON FLUORESCENCE QUENCHING OF QUINOLIN-8-OL - ANALYSIS USING NEGATIVE STERN-VOLMER PLOTS
... and Fluorescence Resonance Energy Transfer (FRET) are three main quenching mechanisms that are caused by external ...hence fluorescence intensity ...the fluorescence intensity ... See full document
8
Fluorescence resonance energy transfer-based real-time polymerase chain reaction method without DNA extraction for the genotyping of F5, F2, F12, MTHFR, and HFE
... show the possibility of performing the genotyping directly from WBCs for F2, F5, F12, MTHFR, and HFE. Thus, under asymmetric concentrations of primers, both the amplification results and fluorescence levels of the ... See full document
8
Single-Molecule Studies Of tRNA Dynamics During Ongoing Translation
... The manner in which EF-G interacts with a PRE state that can occupy classical or hybrid positions has been a subject of intense study; the hybrid state PRE state is believed to play an important role in EF-G induced ... See full document
154
Quantification of Fungal DNA by Using Fluorescence Resonance Energy Transfer and the Light Cycler System
... A quantitative PCR assay with the LightCycler (Roche Di- agnostics, Mannheim, Germany) amplification and detection system was established. This technology combines rapid ther- mocycling with glass capillaries with online ... See full document
5
Extracellular ATP triggers proteolysis and cytosolic Ca2+ rise in Plasmodium berghei and Plasmodium yoelii malaria parasites
... of resonance energy transfer (FRET) peptide hydrolysis (peptide Abz-AIKFFARQ-EDDnp) in the presence of ATP (200 μ M) and the control in isolated (saponin treated) free mixed blood stages of ...(B) ... See full document
11
FRET analysis using sperm activating peptides tagged with fluorescent proteins reveals that ligand binding sites exist as clusters
... these fluorescence tags are 20 times larger than speract, competitive binding experiments using mAmetrine-speract revealed that this FP- speract has binding affinity to the receptor that is comparable ...its ... See full document
8
Association of a Novel Preribosomal Complex in Trypanosoma brucei Determined by Fluorescence Resonance Energy Transfer
... The proteins were detected in the input fraction (positive control, lanes 1, 6, 11, and 16), and the beads alone do not interact nonspecifically with full-length P34 and P34 truncates (negative control, lanes 5, ... See full document
8
Development of a Real Time Fluorescence Resonance Energy Transfer PCR To Detect Arcobacter Species
... the fluorescence resonance energy transfer (FRET) hybridization probe method is very promising since it can detect single nucleotide polymorphisms (SNP) with only one ... See full document
7
Development and Validation of a Quantitative Real Time PCR Assay Using Fluorescence Resonance Energy Transfer Technology for Detection of Aspergillus fumigatus in Experimental Invasive Pulmonary Aspergillosis
... Several key issues regarding the design of assay format, primers, and probes warrant discussion. We purposely avoided the use of a nested PCR approach as a method to increase the sensitivity of the assay because of the ... See full document
7
Fluorescence resonance energy transfer sensors for quantitative monitoring of pentose and disaccharide accumulation in bacteria
... E. coli BL21-Gold(DE3) cells were transformed with pGW1FaraF.Ec, p982 or p3367, which encode an arab- inose FLIP nanosensor with a K d of 200 nM, a maltose FLIP nanosensor with a K d of 25 μM, and a maltose FLIP ... See full document
10
Live-cell imaging with genetically encoded protein kinase activity reporters.
... fluorescent proteins and live- cell imaging techniques enable us to visualize kinase activity in living cells with high spatial and temporal ...(or fluorescence) resonance energy ... See full document
35
Dubbing Modernization: The United States, France, and the Politics of Development in the Ivory Coast, 1946 1968
... Karasawa, Araki et al. 2004; Kawai, Suzuki et al. 2005; Ai, Hazelwood et al. 2008; Ray, De et al. 2008). For example, four-amino-acid recognition sequences, YVAD for caspase-1 and DEVD for caspase-3 were introduced ... See full document
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A reduced graphene oxide-based fluorescence resonance energy transfer sensor for highly sensitive detection of matrix metalloproteinase 2
... enhanced fluorescence spectrum of c-nGO/Pep-FITC sensors after adding the mixture of MMP2 ...the fluorescence intensity, and found that there was hardly any change in the fluorescence intensity of ... See full document
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