[PDF] Top 20 Functional domains within the a sequence involved in the cleavage-packaging of herpes simplex virus DNA.

... The presence of these repeat units in the serially passaged virus stocks was surprising in that, as already noted above, we did not encounter chimeric repeat units containing inserted he[r] ... See full document

... various cleavage and packaging proteins can be proposed based on the results pre- sented in this paper taken together with other ...that cleavage and packaging occur within replication ... See full document

... critical sequence; deletion of this motif had as great an effect as deletion of all of Uc, and the substitution mutant was also significantly ...in packaging than substitutions that did not affect the ... See full document

... of virus penetration into cells at 30°C ...residues involved in oligomerization, for HSV-1 gB and HSV-2 gB, ...repeat sequence in BHV-1 gB ... See full document

... a sequence (41). The presence of these end- specific DNA fragments indicated that all of the tested viruses cleaved concatameric DNA when propagated on Vero cells or G5 cells, respectively ... See full document

... ganglion DNA for detection of viral ...7700 sequence detector (PE Biosystems, Norwalk, ...viral DNA that was serially diluted in 10 ng of mouse genomic DNA (Clontech, Palo Alto, ...Viral ... See full document

... primarily involved criteria based on characteristics of DNA and protein sequences, as distinct from experimental observa- tion of transcripts and proteins or from functional ...coding DNA ... See full document

... LMW DNA ex- tracted from both types of infected cells by Southern blot analysis, using a loxP-a-loxP probe, as previously ...of DNA molecules containing the a signal, previ- ously observed with total ... See full document

... hexons within preassembled ...which DNA synthesis occurs ...globular domains that take up a large portion of the nucleus; however, as has been seen before, replication compartments do not occupy the ... See full document

... the cleavage and pack- aging of herpesvirus ...test packaging signals into an HSV-1 genome lacking the normal resident copies, we have overcome one of the major drawbacks of the previously used amplicon and ... See full document

... Lanes 2 through 18 show restriction enzyme digests of 32P-labeled total DNA from cells infected with standard helper virus control designated J, lanes 3, 7, 8, and 12, or infected with t[r] ... See full document

... The herpes simplex virus type 1 (HSV-1) UL25 gene contains a 580-amino-acid open reading frame that codes for an essential ...1996) involved in virus penetration and capsid assembly ... See full document

... Analysis of origin sequences in herpes simplex virus type 2 HSV-2 has shown that deletion of part of a sequence corresponding to site III in HSV-1 resulted in a dramatic loss in DNA repl[r] ... See full document

... no cleavage takes place in mutants in which assembly is ...mutant virus-infected Vero cells ...these cleavage events may not reflect the exact role of the protease and ICP35 during the virion ... See full document

... possibly involved in oligomerization via a coiled-coil interaction, preceded by a 45-residue unstructured N-terminal ...The sequence alignment reveals this area, and in partic- ular the N-terminal loop ... See full document

... Helix-destabilizing experiments. It has been suggested that the cooperative binding of ICP8 may be important in its helix- destabilizing activity (2, 4). Since the above-described data suggest that the cooperative ... See full document

... genomic DNA from the pooled HSV-BAC recombinants was analyzed without further processing, whereas the pBAC-V1 and pBAC-V2 clones were linearized by digestion with PacI prior to loading onto the ...constructs. ... See full document

... this virus (vBSGFPNES) has undergone a spontaneous deletion ...that virus growth might have been inhibited by the expression of ICP27 containing the NES mu- tation, even when the virus is grown in a ... See full document

... HSV-1 DNA encapsidation remains rel- atively poorly understood, and many fundamental issues, such as the polarity of packaging, remain to be ...during DNA packaging only one terminus, that ... See full document

... the packaging machinery at the portal during and after inser- tion of the genome into the ...the DNA-containing capsid ...viral DNA is arranged in concentric rings with an interlayer spacing of about ... See full document