[PDF] Top 20 Functional expression and evaluation of heterologous phosphoketolases in Saccharomyces cerevisiae
Has 10000 "Functional expression and evaluation of heterologous phosphoketolases in Saccharomyces cerevisiae" found on our website. Below are the top 20 most common "Functional expression and evaluation of heterologous phosphoketolases in Saccharomyces cerevisiae".
Functional expression and evaluation of heterologous phosphoketolases in Saccharomyces cerevisiae
... endogenous phosphatases involved in glycerol biosynthe- sis, as suggested by a patent filed by the company Amyris (Hawkins et al. 2014), which allowed us to determine their individual contribution to the degradation of ... See full document
13
Functional Heterologous Protein Expression by Genetically Engineered Probiotic Yeast Saccharomyces boulardii
... Sequence analysis of the URA3 open reading frame in S. boulardii Mutants 1–3 revealed single amino acid substitutions located outside of specific functional domains (Fig. 4A). M1 and M3 both contained an A160S ... See full document
12
DNA-Directed Expression of Functional Flock House Virus RNA1 Derivatives in Saccharomyces cerevisiae, Heterologous Gene Expression, and Selective Effects on Subgenomic mRNA Synthesis
... in Saccharomyces cerevisiae, yeast DNA plasmids were constructed to inducibly express wild-type FHV RNA1 in ...gene expression from FHV subgenomic ... See full document
10
Comparison of heterologous xylose transporters in recombinant Saccharomyces cerevisiae
... a functional screening step ...by expression of a P. stipitis cDNA library in a S. cerevisiae HXT null strain and subsequent reconstitution of growth on glucose ...S. cerevisiae HXT null ... See full document
7
Heterologous expression of an α-amylase inhibitor from common bean (Phaseolus vulgaris) in Kluyveromyces lactis and Saccharomyces cerevisiae
... for expression in yeasts (αAI‑OPT) and cloned into the protein expression vectors pKLAC2 and ...and Saccharomyces cerevisiae YPH499 were transformed with the optimised genes and transformants ... See full document
13
A study on the use of strain-specific and homologous promoters for heterologous expression in industrial Saccharomyces cerevisiae strains
... or functional character- istics show higher levels of expression variation across strains, either in response to selection (Tirosh et ...the expression differences between the promoters of strains ... See full document
11
Heterologous Expression Studies of Saccharomyces cerevisiae Reveal Two Distinct Trypanosomatid CaaX Protease Activities and Identify Their Potential Targets
... Both of the trypanosomal CaaX proteases are functional enzymes. The yeast CaaX proteases promote yeast mating, ostensibly through their ability to proteolyse the isoprenylated yeast a-factor precursor (3). CaaX ... See full document
10
Heterologous biosynthesis and manipulation of crocetin in Saccharomyces cerevisiae
... biosynthesis, heterologous crtZ and ccd were codon optimized and introduced into an existing β-carotene producer ...(S. cerevisiae SyBE_ SC0014CY06), which processed endogenous ALDs to catalyze the final ... See full document
14
Engineering Acetyl Coenzyme A Supply: Functional Expression of a Bacterial Pyruvate Dehydrogenase Complex in the Cytosol of Saccharomyces cerevisiae
... S. cerevisiae expands the options for metabolic engineering strat- egies aimed at optimizing energetics of cytosolic acetyl-CoA syn- thesis in this ...S. cerevisiae equals 1 ATP per one molecule of ...the ... See full document
11
Marine metagenomics: strategies for the discovery of novel enzymes with biotechnological applications from marine environments
... for functional activities ...Gene Expression screening (SIGEX) have facilitated the cloning of catabolic operons potentially involved in benzoate and catechol degradation among others ...These ... See full document
8
Nucleoporin FG Domains Facilitate mRNP Remodeling at the Cytoplasmic Face of the Nuclear Pore Complex
... rat8-2 (dbp5) nup42DFG nup159DFG triple mutant to ana- lyze whether other FG domains can functionally compensate when anchored to the same location in the NPC. A series of “FG swap” expression vectors were ... See full document
21
A versatile vector system for generating recombinant EGFP tagged proteins in yeast
... Five micrograms of the plasmid pBS(GFP) were di- gested with Nco I and Not I overnight. The insert was filled with T4 DNA polymerase in the presence of dNTPs. Meanwhile, 5 micrograms of the plasmid pFL61 [18], was ... See full document
7
A Bioenergetic Redox Approach to the Effect of Live Yeast on Ruminal pH during Induced Acidosis in Dairy Cow
... The objective of this study was to evaluate the capacity of the live yeast (LY) Saccharomyces cerevisiae in optimizing ruminal pH and in understanding its mode of action during induced acidosis in dairy ... See full document
9
Heterologous Expression of Membrane and Soluble Proteins Derepresses GCN4 mRNA Translation in the Yeast Saccharomyces cerevisiae
... Unfortunately, heterologous expression of mem- brane proteins is very difficult and by no way routine ...of heterologous protein in the membrane is grossly independent of the applied host, pointing ... See full document
14
Regulation of the Target of Rapamycin Signaling Pathway in Saccharomyces cerevisiae
... I first determined the functionality of GFP-tagged Far fusion proteins by a plate halo assay (Fig. 1A). Wild-type mating type a cells normally arrest cell growth around a paper disc infused with α-factor and create a ... See full document
170
Functional expression and characterization of five wax ester synthases in Saccharomyces cerevisiae and their utility for biodiesel production
... little is known about the substrate specificity of WSs and some WSs did not even possess significant WS activity when expressed in recombinant S. cerevisiae [10]. Here, we tested the activity of the five WSs with ... See full document
10
Env7p Associates with the Golgin Protein Imh1 at the trans-Golgi Network in Candida albicans
... CaEnv7 is localized to the trans-Golgi. Fluorescence of a green fluorescent protein (GFP)-tagged CaEnv7 (Env7-GFP) strain using an endogenous promoter remained undetected. Env7 with a C-terminally tagged GFP and expressed ... See full document
15
In vivo DNA expression of functional brome mosaic virus RNA replicons in Saccharomyces cerevisiae.
... these expression plasmids lack 5 9 - and 3 9 -terminal cis-acting RNA replication signals of BMV RNA1 and RNA2 (31) and consequently are not substrates for BMV RNA ... See full document
10
TERribly Difficult: Searching for Telomerase RNAs in Saccharomycetes
... Abstract: The telomerase RNA in yeasts is large, usually > 1, 000 nt, and contains functional elements that have been extensively studied experimentally in several disparate species. Nevertheless, they are very ... See full document
14
Nodamura Virus RNA Replication in Saccharomyces cerevisiae: Heterologous Gene Expression Allows Replication-Dependent Colony Formation
... Nodamura virus (NoV) and Flock House virus (FHV) are members of the family Nodaviridae. The nodavirus genome is composed of two positive-sense RNA segments: RNA1 encodes the viral RNA-dependent RNA polymerase and RNA2 ... See full document
8
Related subjects