[PDF] Top 20 A herpes simplex virus type 1 mutant lacking the ICP0 introns reactivates with normal efficiency.

... 5569 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Previous evidence suggests that the latency-associated transcript LAT gene of herpes simplex virus type 1 to have a[r] ... See full document

... of ICP0 has been shown to reduce the activity of ICP0 in several other assays ...of ICP0. The 29-fold reduction in ICP0 activity observed for n518Bac relative to the wild type may be a ... See full document

... plaquing efficiency of dl1403 but not wild-type virus are augmented in cells that lack PML or Sp100 ...plaquing efficiency of wild-type VZV are un- affected by depletion of Sp100 and ... See full document

... before ICP0 translocates into the cytoplasm (21, ...of ICP0 to translocate in this ICP27- dependent manner abrogates its assembly into the virion ...for ICP0 assembly (43). Once in the cytoplasm, ... See full document

... Herpes simplex virus type 1 (HSV-1) mutants impaired in the activities of the structural protein VP16 and the immediate-early (IE) proteins ICP0 and ICP4 establish a ... See full document

... ICP0-null mutant HSV-1 does not have an improved plaque- forming efficiency in HFs deficient in ...and ICP0- null mutant HSV-1 on naı¨ve HFs and HF-PIV5V cells depleted of ... See full document

... wild-type herpes simplex virus type 1 (HSV-1), ICP0-null mutant viruses reactivate inefficiently from explanted, latently infected mouse trigeminal ganglia ... See full document

... In an attempt to determine whether the growth status of cells in the monolayer affects the ability of ICPO mutants to initiate plaque formation, the plating efficiencies and abilities of[r] ... See full document

... of ICP0 null mutants is highly multiplicity de- pendent (7, 8, 18, ...in ICP0 null mutant-infected cells relative to wild-type virus-in- fected cells, whereas at high multiplicities, ... See full document

... Expression of herpes simplex virus type 1 latency-associated transcripts in the trigeminal ganglia of mice during acute infection and reactivation of latent infection.. Expression of her[r] ... See full document

... (i) ICP0, Sp100, hDaxx, and PML colocalize in both preexisting ND10 and novel ND10-like structures at the early stages of infection; (ii) ICP0 brings about the destruction of these foci; (iii) endogenous ... See full document

... affect ICP0’s subcellular and subnuclear localization (Phos 3), ND10-dis- rupting activity in a subset of cells (Phos 2 and 3), and trans- activating activity (Phos 1 and 3) in Vero ...Phos mutant ... See full document

... which ICP0 achieves these functions are very poorly ...infection, ICP0 transiently localizes to discrete subnuclear structures, known as ND10 or PODs, and as a consequence the cellular ND10 antigens are ... See full document

... (Fig. 1) nevertheless fail to ac- cumulate detectable levels of unspliced ␣-globin ...ICP27 mutant, S23, that is de- fective in gene activation (52). This mutant, as well as several other mutants in ... See full document

... their normal roles in cellular pathways, have properties that inhibit virus ...nucleus. ICP0-induced degradation of the core ND10 component PML and selected isoforms of Sp100 (12, 21) provides a ... See full document

... increases ICP0-null mu- tant HSV-1 replication efficiency in a human hepatocyte cell ...for ICP0-null mutant HSV-1 infection than cells depleted of either protein alone, the ... See full document

... of ICP0 are similar to those of the HDAC inhibitor trichostatin A (29, 59), but as quiescence is established, inhibition of histone deacety- lases is not sufficient to activate gene expression ...ever, ICP0 ... See full document

... Using GST-ICPO fusion protein affinity chromatography and in vitro-synthesized [35S]Met-labeled ICPO and ICP4, ICP4 was shown to interact preferentially with the fusion protein containin[r] ... See full document

... cells. Herpes simplex virus type 1 (HSV-1) replication is somewhat reduced in tissue culture in the presence of IFN, presumably due to decreased viral ...product ICP0 ... See full document

... of virus replication (20), since the disruption of ND10s which occurs during HSV infection correlates with ICP0 and protea- some-dependent degradation of several PML isoforms ...assays ICP0 ... See full document