[PDF] Top 20 Role of the Herpes Simplex Virus Helicase-Primase Complex during Adeno-Associated Virus DNA Replication
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Role of the Herpes Simplex Virus Helicase-Primase Complex during Adeno-Associated Virus DNA Replication
... AAV DNA replication but retained the ability to mediate nuclear colocalization of ICP8 and Rep ...AAV DNA, and the other HSV helper proteins, rather than primase catalytic activity, that is ... See full document
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Role of the Adenovirus DNA-Binding Protein in In Vitro Adeno-Associated Virus DNA Replication
... AAV DNA replication. The restoration of replication capacity by the addition of either Ad-DBP or RPA (both produced in expression systems) to the immunodepleted extract demonstrated that only a ... See full document
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A Role for Single-Stranded Templates in Cell-Free Adeno-Associated Virus DNA Replication
... single-stranded replication templates are produced by a mechanism other than strand displacement ...as replication templates were pro- duced by a mechanism other than strand displacement repli- ... See full document
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Complete In Vitro Reconstitution of Adeno-Associated Virus DNA Replication Requires the Minichromosome Maintenance Complex Proteins
... a role in AAV DNA replication, but cdc45, Cdt1, MCM10, and Smc1 are known to be associated with the active MCM2 to -7 complex during eukaryotic chromosome replica- tion (reviewed ... See full document
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Regulation of Adeno-Associated Virus DNA Replication by the Cellular TAF-I/Set Complex
... TAF-I/Set complex actively participate in AAV-2 ...TAF-I/Set complex binds nucleo- somes and was originally shown to inhibit transcription by masking histones from being acetylated, hence the original name ... See full document
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Herpes Simplex Virus Type 1 Helicase-Primase: DNA Binding and Consequent Protein Oligomerization and Primase Activation
... circular DNA molecule with an annealed replication ...coli replication pro- teins, it has been possible to reconstitute the synthesis of both the leading and lagging strands and to demonstrate that ... See full document
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Impact of the MRN Complex on Adeno-Associated Virus Integration and Replication during Coinfection with Herpes Simplex Virus 1
... ds- DNA breaks (DSB) or in general on detection of abnormal DNA structures such as stalled DNA replication forks ...the DNA lesion, MRN can initiate the DNA damage re- sponse ... See full document
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The catalytic subunit of the DNA polymerase of herpes simplex virus type 1 interacts specifically with the C terminus of the UL8 component of the viral helicase-primase complex.
... other DNA replication systems it is consid- ered likely that initiation of HSV-1 DNA synthesis involves the formation of an initiation complex at one or more of the replication ...viral ... See full document
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Mutations in the Putative Zinc-Binding Motif of UL52 Demonstrate a Complex Interdependence between the UL5 and UL52 Subunits of the Human Herpes Simplex Virus Type 1 Helicase/Primase Complex
... Herpes simplex virus type 1 (HSV-1) encodes a heterotrimeric helicase-primase (UL5/8/52) ...in helicase superfamily 1, and UL52 contains conserved motifs found in ...the ... See full document
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An Intertypic Herpes Simplex Virus Helicase-Primase Complex Associated with a Defect in Neurovirulence Has Reduced Primase Activity
... this virus of an intertypic helicase-primase complex containing subunits of both HSV-1 and HSV-2 ...recombinant virus was con- structed by replacement of only the HSV-1 UL5 gene with ... See full document
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DNA-Binding Activity of Adeno-Associated Virus Rep Is Required for Inverted Terminal Repeat-Dependent Complex Formation with Herpes Simplex Virus ICP8
... seven herpes simplex virus (HSV)- encoded replication functions was shown to provide helper activity for productive replication of adeno-associated virus ...meric ... See full document
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Rep-Dependent Initiation of Adeno-Associated Virus Type 2 DNA Replication by a Herpes Simplex Virus Type 1 Replication Complex in a Reconstituted System
... full-length DNA chains that are essentially free of nicks ...of replication products migrates in the first di- mension at a position consistent with their being mAAV ...migration during denaturing ... See full document
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Identification of Rep-Associated Factors in Herpes Simplex Virus Type 1-Induced Adeno-Associated Virus Type 2 Replication Compartments
... of DNA polymerases. The MSH2/3 complex (MutS  ) recog- nizes longer insertion/deletion loops generated, notably, dur- ing replication of microsatellite regions ...cellular DNA damage response ... See full document
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Inhibition of Herpes Simplex Virus Type 1 Replication by Adeno-Associated Virus Rep Proteins Depends on Their Combined DNA-Binding and ATPase/Helicase Activities
... helper virus for productive replication inevitably leads to competition for cellular resources as well as for the helper factors themselves, which often are essential for both AAV and the helper ...the ... See full document
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Inhibition of Herpes Simplex Virus Replication by a 2-Amino Thiazole via Interactions with the Helicase Component of the UL5-UL8-UL52 Complex
... a complex that displays DNA-dependent ATPase, he- licase, and primase activities that are identical to those pro- duced during a herpesvirus infection allowed functional and biochemical ... See full document
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Origin of adeno-associated virus DNA replication is a target of carcinogen-inducible DNA amplification.
... To analyze whether the left-hand AAV fragment containing the AAV-2 origin/TR structure is able to promote replication activity in carcinogen-treated cells in the absence of parvoviral ge[r] ... See full document
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Isolation of the viral DNA replication complex from adeno-associated virus type 1-infected cells.
... The reaction mixture 100 pi contained 50 mM Trishydrochloride, pH 7.9, 2.5 mM MgC12, 2 mM DTT, 444 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The replication compl[r] ... See full document
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High-Frequency Intermolecular Homologous Recombination during Herpes Simplex Virus-Mediated Plasmid DNA Replication
... recombined DNA revealed that the majority of these IM-HR events resulted in sequence replacement, while the minority resulted in sequence ...against during growth in ... See full document
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Role of Cellular Phosphatase cdc25C in Herpes Simplex Virus 1 Replication
... Cell infection. Cultures of the indicated cell type were infected with the appropriate virus at an appropriate multiplicity of infection in medium 199V (mixture 199 supplemented with 1% calf serum) on a rotary ... See full document
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Analysis of the Role of Autophagy in Replication of Herpes Simplex Virus in Cell Culture
... To establish productive infections, viruses have a number of genes which inhibit the establishment of the host antiviral state and subvert host metabolic pathways to facilitate and amplify their own replication. ... See full document
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