Top PDF Human Cytomegalovirus UL135 and UL136 Genes Are Required for Postentry Tropism in Endothelial Cells
Human Cytomegalovirus UL135 and UL136 Genes Are Required for Postentry Tropism in Endothelial Cells
... both UL135 and UL136, and further work is re- quired to understand how these proteins function cooperatively to maintain membrane organization for VAC ..., UL135 STOP , or UL136 GalK infection ...
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Human Cytomegalovirus Tropism for Endothelial Cells: Not All Endothelial Cells Are Created Equal
... those required for replication in ECs may be necessary to modulate aspects of the virus infection process to facilitate long-term persistent, instead of acute cytolytic, replication in this cell ...be ...
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The US16 Gene of Human Cytomegalovirus Is Required for Efficient Viral Infection of Endothelial and Epithelial Cells
... epithelial cells seems to overlap that of clinical isolates of HCMV with targeted mutations in the pUL genes that enable entry into these cell types (18, ...epithelial cells can be excluded by the ...
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Human Cytomegalovirus UL131 Open Reading Frame Is Required for Epithelial Cell Tropism
... epithelial cells, endothelial cells, or fibroblasts were transferred into 12-well plates and incubated overnight to produce subconfluent ...infected cells were identified by GFP expression and ...
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Human Cytomegalovirus UL131-128 Genes Are Indispensable for Virus Growth in Endothelial Cells and Virus Transfer to Leukocytes
... 128 genes were supplied in trans by retrovirus-mediated trans- duction of ...single genes could rescue the growth and/or transfer properties only when the complementing gene(s) matched the affected gene of ...
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Mutational Mapping of pUL131A of Human Cytomegalovirus Emphasizes Its Central Role for Endothelial Cell Tropism
... EC tropism in the ccta72-75 mutant was associated with reduced incorporation of the three UL128-131A proteins indi- cates that the degree of EC tropism might be regulated by the amount of gH/gL/pUL128-131A ...
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Charge Cluster-to-Alanine Scanning of UL128 for Fine Tuning of the Endothelial Cell Tropism of Human Cytomegalovirus
... region interacts with cellular surface structures. Ryckman et al. have demonstrated direct interactions of UL128 with both gL and UL130, each of which bound independently to gH (22). This implies that loss of one ...
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Hemagglutinin-Dependent Tropism of H5N1 Avian Influenza Virus for Human Endothelial Cells
... Future studies are required to confirm the biological impor- tance of our findings. To our knowledge, there is only one immunohistochemistry study available describing the absence of viral RNA or NP in EDC in two ...
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Efficient Lytic Infection of Human Arterial Endothelial Cells by Human Cytomegalovirus Strains
... 2000 Endothelial cells (EC) are common targets of permissive infection by human cytomegalovirus (HCMV) in vivo during acute ...cell tropism rather than the vascular bed of origin of ...
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Human Cytomegalovirus Entry into Epithelial and Endothelial Cells Depends on Genes UL128 to UL150 and Occurs by Endocytosis and Low-pH Fusion
... of cells infected by TR ⌬4 after PEG treatment was 60% to 70%, compared with 98% for ...Therefore, cells would not be synchronously infected and com- parison of virus yield would be ...UL128-to-UL150 ...
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Expression of human cytomegalovirus UL36 and UL37 genes is required for viral DNA replication.
... of the UL36 and UL37 unspliced RNA to show that these gene products are required for HCMV DNA replication. Southern analysis showed that this oligonucleotide almost completely inhibits HCMV DNA rep- lication when ...
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Pathogen at the Gates: Human Cytomegalovirus Entry and Cell Tropism
... Figure 2. Regulation of alternative gH/gL complexes by UL148 and US16. UL148, a viral endoplasmic reticulum (ER)-resident glycoprotein, promotes high-level expression of the trimer during infection (wild-type) by ...
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Viral Regulation of RANTES Expression during Human Cytomegalovirus Infection of Endothelial Cells
... and endothelial cells is required for high-level RANTES ...with endothelial cells, signals are relayed by the cell to broadcast foreign ...
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Inactivation of the Human Cytomegalovirus US20 Gene Hampers Productive Viral Replication in Endothelial Cells
... IMPORTANCE Human cytomegalovirus (HCMV) is a major pathogen in newborns and immunocompromised ...target cells, including endothelial cells, which represent a key target for viral ...
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Human Cytomegalovirus Genes in the 15-Kilobase Region Are Required for Viral Replication in Implanted Human Tissues in SCID Mice
... RESULTS The SCID-hu mouse is a unique model for studying the differences between attenuated and virulent strains. To con- firm the attenuation of strain AD169 and the virulence of strain Toledo in vivo, we first ...
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Characterization of the Human Cytomegalovirus gH/gL/UL128-131 Complex That Mediates Entry into Epithelial and Endothelial Cells
... and endothelial cells ...into endothelial cells (51; ...both endothelial and epithelial ...UL128-131 genes during replication and spread on fibro- ...
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The Human Cytomegalovirus Ribonucleotide Reductase Homolog UL45 Is Dispensable for Growth in Endothelial Cells, as Determined by a BAC-Cloned Clinical Isolate of Human Cytomegalovirus with Preserved Wild-Type Characteristics
... including endothelial cells ...an endothelial cell-tropic HCMV would induce apoptosis and lose tropism for endothelium if the M45 homolog UL45 was ...
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Human Cytomegalovirus Persistently Infects Aortic Endothelial Cells
... In the present study, we examined characteristics of HCMV replication in AEC. We found that HCMV infection was not lytic and resulted in the accumulation of significant amounts of extracellular but not intracellular ...
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Upregulation of CD40 Expression on Endothelial Cells Infected with Human Cytomegalovirus
... Our data clearly show that the expression of CD40 on the surface of EC is enhanced following infection with HCMV and that subsequent stimulation by CD154 activates EC to express adhesion molecules. The increase in CD40 ...
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Capsid-Dependent and -Independent Postentry Restriction of Primate Lentivirus Tropism in Rodent Cells
... the postentry, preintegration phase of the lentivirus life cycle in rodent ...3T3 cells, resistance appears to be due predominantly to the presence of a saturable, capsid-targeting inhibitor of ...of ...
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