[PDF] Top 20 Human immunodeficiency virus integration in a cell-free system.
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Human immunodeficiency virus integration in a cell-free system.
... To determine whether any nucleotides or other small molecules were required as cofactors for HIV integration, the crude cytoplasmic extract from infected cells was fractionated by gel ex[r] ... See full document
5
Primary Cell Model for Activation-Inducible Human Immunodeficiency Virus
... Impact of NF- B on HIV activation. Induction of the viral LTR is tightly regulated in our in vitro model system. We therefore determined if our model could be used to assess the importance of various LTR-binding ... See full document
11
Effect of Latent Human Immunodeficiency Virus Infection on Cell Surface Phenotype
... infected cell enters a quiescent ...the cell would then appear similar to an uninfected T ...latent virus in vivo, characterization of the latent reservoir at the cellular level has been ...SCID-hu ... See full document
9
Human Immunodeficiency Virus Persistence and Production in T-Cell Development
... detection system in a 25-l reaction volume for 40 cycles between 95°C for 15 seconds and 60°C for 1 min, using the components in the TaqMan core reagent kit (Applied Biosystems, Foster City, ...the human ... See full document
9
Inducible human immunodeficiency virus type 1 packaging cell lines.
... vector virus transfer, drug-resistant HeLaT4 tar- get cells were cloned and the genomic DNA isolated from them was subjected to Southern ...GPT-resistant cell clones contained HVP, HSN, and HIV- gpt vector ... See full document
8
Altered Gag Polyprotein Cleavage Specificity of Feline Immunodeficiency Virus/Human Immunodeficiency Virus Mutant Proteases as Demonstrated in a Cell-Based Expression System
... feline immunodeficiency virus (FIV) protease (PR) as a mutational system to study the molecular basis of substrate-inhibitor specificity for lentivirus PRs, with a focus on human ... See full document
12
Mouse T cells restrict replication of human immunodeficiency virus at the level of integration
... not human T-cells, (ii) a lack of correlative evaluations of consecutive replica- tion steps, (iii) a lack of control for residual proviral plas- mid contamination in virus inocula, and (iv) quantification ... See full document
16
Antigenic Properties of the Human Immunodeficiency Virus Transmembrane Glycoprotein during Cell-Cell Fusion
... the system was returned to the fusogenic temper- ature of 37°C ...by cell-cell contact ...our cell-cell fusion system is highly efficient and therefore inherently resistant to ... See full document
12
Integration Targeting by Avian Sarcoma-Leukosis Virus and Human Immunodeficiency Virus in the Chicken Genome
... novo integration and ERVs in the chicken ...ASLV integration sites was quite different from that of related ERVs, suggesting mod- els for the forces determining which ERVs persist in the ge- ...of ... See full document
10
Integration is not necessary for expression of human immunodeficiency virus type 1 protein products.
... In addition, in all three cell types used in this study peripheral blood lymphocytes, Mo-T, and MT-4, infection with wild-type HIV-1 mfA virions and integrase mutants resulted in the syn[r] ... See full document
5
A Sensitive, Quantitative Assay for Human Immunodeficiency Virus Type 1 Integration
... host cell genome. Human immunodeficiency virus type 1 (HIV-1) pro- viruses appear to contribute to viral ...of integration events in quiescent leukocytes. Assays for HIV-1 ... See full document
9
Cell-Free versus Cell-to-Cell Infection by Human Immunodeficiency Virus Type 1 and Human T-Lymphotropic Virus Type 1: Exploring the Link among Viral Source, Viral Trafficking, and Viral Replication
... donor cell and not in the target cell in the case of the FIG 2 Infection after cell-cell contact: the viral ...donor cell (left) and the target cell ...1, cell-cell ... See full document
11
Rapid induction of apoptosis by cell-to-cell transmission of human immunodeficiency virus type 1.
... acquired immunodeficiency, which predisposes the patient to lethal infectious and neoplastic complications of AIDS and for which no therapy is ...Programmed cell death has been postulated as a potential ... See full document
9
Impaired Accessory Cell Function in a Human Dendritic Cell Line after Human Immunodeficiency Virus Infection
... generated human dendritic cell (DC) hybridoma cell lines by fusing HGPRT-deficient promonocytic U937 cells with immature DCs obtained by culturing peripheral blood monocytes with interleukin-4 (IL-4; ... See full document
12
Human Immunodeficiency Virus Integration Efficiency and Site Selection in Quiescent CD4+ T Cells
... are integration competent (60, ...activated cell populations that returned to a resting state after stimulation, such as memory T cells (31, ...cells), integration efficiency was slightly decreased ... See full document
12
Antigenic Properties of the Human Immunodeficiency Virus Envelope during Cell-Cell Fusion
... or cell-based immunogens supports this concept (5, ...inhibit virus-mediated membrane ...of cell-cell or virus-cell membrane ...standard cell fusion system using ... See full document
10
Efficiency of Cell-Free and Cell-Associated Virus in Mucosal Transmission of Human Immunodeficiency Virus Type 1 and Simian Immunodeficiency Virus
... to free virus or cell-associated virus. Cell-free replication-competent HIV-1 (R5) ...(DDAO-SE)-stained human CD4 ⫹ T lymphocytes infected with HIV-1 (R5) ... See full document
9
Discontinuous plus-strand DNA synthesis in human immunodeficiency virus type 1-infected cells and in a partially reconstituted cell-free system.
... FIG. 5. Cell-free plus-strand synthesis from non-PPT sites. DNA synthesis reactions were carried out for 40 min as described in Materials and Methods and the legends to Fig. 3 and 4. Plus-strand initiation ... See full document
11
Active and Selective Transcytosis of Cell-Free Human Immunodeficiency Virus through a Tight Polarized Monolayer of Human Endometrial Cells
... of free and cell-associated HIV-1 across a monolayer of epithelial ...whether cell-associated R5- and X4-tropic viruses, as well as the corre- sponding free viral particles, were capable of ... See full document
5
Cell-Free Human Immunodeficiency Virus Type 1 Transcytosis through Primary Genital Epithelial Cells
... the cell pellet was digested in 1 mg/ml of collagenase-dispase (Roche Molecular Biochemicals) containing 1 mg/ml of DNase (Sigma) and ...fluorescence-activated cell sorting (FACS) as previously de- scribed ... See full document
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