[PDF] Top 20 Human Immunodeficiency Virus Type 1 Drug Resistance Testing: a Comparison of Three Sequence Based Methods
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Human Immunodeficiency Virus Type 1 Drug Resistance Testing: a Comparison of Three Sequence Based Methods
... determining drug resistance in human immunodeficiency virus (HIV) type 1 (HIV-1)-infected patients is ...drug resistance. Two genotypic tests, the ... See full document
9
Genetic Diversity of Protease and Reverse Transcriptase Sequences in Non Subtype B Human Immunodeficiency Virus Type 1 Strains: Evidence of Many Minor Drug Resistance Mutations in Treatment Naive Patients
... Phylogenetic and sequence analyses. The genetic subtypes were determined by phylogenetic tree analysis. The new nucleotide sequences and the sequences of the protease and RT genes from reference strains ... See full document
7
Multicenter comparison of three commercial methods for quantification of human immunodeficiency virus type 1 RNA in plasma
... of human immunodeficiency virus type 1 (HIV-1) infection, such as the CD4 1 lymphocyte count, p24 antigenemia, and quantitative plasma and peripheral blood mononuclear ... See full document
7
High Sequence Conservation of Human Immunodeficiency Virus Type 1 Reverse Transcriptase under Drug Pressure despite the Continuous Appearance of Mutations
... of sequence conservation in human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) in vivo, the first 320 amino acids of RT obtained from 2,236 ... See full document
12
Human Immunodeficiency Virus Type 1 Subtype F Reverse Transcriptase Sequence and Drug Susceptibility
... matrix based on 591 sites was generated with the DNADIST program of the PHYLIP package, version ...JRCSF), three subtype D sequences (ELI, NDK, and Z2Z6), two subtype A sequences (UG037 and U455), the ... See full document
5
Prevalence of Human Immunodeficiency Virus Drug Resistance Mutations and Subtypes in Drug Naive, Infected Individuals in the Army Health Service of Rio de Janeiro, Brazil
... Patients infected with B and non-B strains were monitored for up for 32 months (median follow-up time, 19.5 months) by using CD4 cell counts and VL measures as markers. T-cell- number determinations were performed in 50 ... See full document
5
Augmentation of Human Immunodeficiency Virus Type 1 Subtype E (CRF01_AE) Multiple-Drug Resistance by Insertion of a Foreign 11-Amino-Acid Fragment into the Reverse Transcriptase
... A human immunodeficiency virus type 1 (HIV-1) subtype E (CRF01_AE) variant (99JP-NH3-II) possessing an in-frame 33-nucleotide insertion mutation in the  3-  4 loop coding ... See full document
10
Human Immunodeficiency Virus Type 1 Cloning Vectors for Antiretroviral Resistance Testing
... clinical resistance testing involve simplified cloning to facilitate better detection of minorities of resistant virus and linkage of multi- ple resistance ...recombinant virus vectors ... See full document
9
Comparative Study of Methods for Detecting Sequence Compartmentalization in Human Immunodeficiency Virus Type 1
... available methods for subsequent molecular ...rigorous comparison between cited methodol- ogies is ...different methods to detect compartmentalization frequently ...of methods, we benchmarked ... See full document
9
Fitness Comparison of Thymidine Analog Resistance Pathways in Human Immunodeficiency Virus Type 1
... ZDV resistance mutations were competed against wild-type virus ...wild-type virus in the absence of drug but had a fitness advantage at ZDV concentrations of ...was based ... See full document
8
Comparison of Predicted Scaffold-Compatible Sequence Variation in the Triple-Hairpin Structure of Human Immunodeficiency Virus Type 1 gp41 with Patient Data
... is based on the recently published FASTER algorithm (17), a new powerful high-throughput algorithm for side chain placement ...Several methods to predict the response of a protein to point mutations have ... See full document
12
Molecular Characterization of Human Immunodeficiency Virus Type 1 (HIV 1) and HIV 2 in Yaoundé, Cameroon: Evidence of Major Drug Resistance Mutations in Newly Diagnosed Patients Infected with Subtypes Other than Subtype B
... NRTI resistance mutations were observed as wild-type sequences in three CRF02_AG (T215Y/F) and one CRF13_cpx (Y118C) ...confers resistance to ZDV in nearly all HIV-1 isolates, whereas ... See full document
8
Effects of Human Immunodeficiency Virus Type 1 Resistance to Protease Inhibitors on Reverse Transcriptase Processing, Activity, and Drug Sensitivity
... Human immunodeficiency virus type 1 (HIV-1) variants resistant to protease inhibitors often display a reduced replicative capacity as a result of an impairment of protease ...In ... See full document
5
Comparison of three nonradioisotopic polymerase chain reaction based methods for detection of human immunodeficiency virus type 1
... The two false-positive results found with the Roche method were apparently not the result of sample lysate contamination, since subsequent amplification and detection by other methods di[r] ... See full document
9
Evaluation of Dried Blood Spots for Human Immunodeficiency Virus Type 1 Drug Resistance Testing
... had drug resis- tance mutations which allowed us to compare the genotypic profiles between plasma and ...the resistance profiles from plasma and DBS (Table ...the three mutations that were absent in ... See full document
5
Sources and Magnitude of Intralaboratory Variability in a Sequence Based Genotypic Assay for Human Immunodeficiency Virus Type 1 Drug Resistance
... phenotyping methods should be a recognized limitation of clinical antiretroviral drug resistance testing (4, ...of resistance testing assays for detection of minority species in ... See full document
8
Novel Enzyme Linked Minisequence Assay for Genotypic Analysis of Human Immunodeficiency Virus Type 1 Drug Resistance
... of human immunodeficiency virus type 1 (HIV-1) treatment failure is the emergence of drug- resistant viruses (10, 12, ...anti-HIV-1 drug induces a specific ... See full document
9
Increased Multinucleoside Drug Resistance and Decreased Replicative Capacity of a Human Immunodeficiency Virus Type 1 Variant with an 8-Amino-Acid Insert in the Reverse Transcriptase
... Phenotypic drug resistance ...the drug resistance pheno- type (Fig. 1); one pretherapy sample (3 May 1994), one sample taken during ZDV monotherapy (21 March 1995), and two ... See full document
8
Performance of the Affymetrix GeneChip HIV PRT 440 Platform for Antiretroviral Drug Resistance Genotyping of Human Immunodeficiency Virus Type 1 Clades and Viral Isolates with Length Polymorphisms
... to sequence HIV-1 clade B pol cDNAs that lack length polymorphisms ...is based on the confirmation of sequence by hybridization of the sample to the clade B-defined oligonucleotide array on ... See full document
5
Modification of a loop sequence between α helices 6 and 7 of virus capsid (CA) protein in a human immunodeficiency virus type 1 (HIV 1) derivative that has simian immunodeficiency virus (SIVmac239) vifand CA α helices 4 and 5 loop improves replication in cynomolgus monkey cells
... adapted virus has not yet been fully characterized; so it may be that fur- ther modifications of the viral genome are necessary for optimal replication of HIV-1 genomes in OWM ...incoming virus by ... See full document
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