[PDF] Top 20 Identification of Rep-Associated Factors in Herpes Simplex Virus Type 1-Induced Adeno-Associated Virus Type 2 Replication Compartments
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Identification of Rep-Associated Factors in Herpes Simplex Virus Type 1-Induced Adeno-Associated Virus Type 2 Replication Compartments
... AAV replication resulted in the identification of a single adenoviral helper protein ...AAV replication than that of adenovirus. Among the helper factors retrieved in our study were the ICP8, ... See full document
17
Live Covisualization of Competing Adeno-Associated Virus and Herpes Simplex Virus Type 1 DNA Replication: Molecular Mechanisms of Interaction
... a rep-negative rAAV2, inhibits HSV-1 DNA ...amplicon replication in the presence of rAAV DNA and AAV rep also holds true for the respective wt viruses, we set out to analyze the influence of ... See full document
12
Herpes Simplex Virus Type 1 ICP0 Protein Mediates Activation of Adeno-Associated Virus Type 2 rep Gene Expression from a Latent Integrated Form
... the identification of the HSV-1 factors necessary to relieve the repressive state of the AAV p5 ...the virus is still replication competent but grows poorly and also reactivates from ... See full document
10
Adeno-Associated Virus Type 2 Modulates the Host DNA Damage Response Induced by Herpes Simplex Virus 1 during Coinfection
... in virus-infected cells and mock-infected cells ...FIG 1 DNA damage signaling induced by viral ...AAV2 Rep was used as an infection ... See full document
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Impact of the Interaction between Herpes Simplex Virus Type 1 Regulatory Protein ICP0 and Ubiquitin-Specific Protease USP7 on Activation of Adeno-Associated Virus Type 2 rep Gene Expression
... of rep gene expression by ICP0 in transfected and infected ...activate rep gene expression in trans- fected cells and to activate HSV-1 early gene expression effi- ciently during ...of rep ... See full document
5
Herpes Simplex Virus Type 1/Adeno-Associated Virus Hybrid Vectors Mediate Site-Specific Integration at the Adeno-Associated Virus Preintegration Site, AAVS1, on Human Chromosome 19
... VERO 2-2 cells (33), human 293 cells, and the Detroit 6 cell line 7374 (4), which is latently infected with wild-type AAV2, were maintained in Dulbecco’s modified Eagle medium supplemented with 10% ... See full document
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Intermediates of adeno-associated virus type 2 assembly: identification of soluble complexes containing Rep and Cap proteins.
... of Rep and Cap proteins in AAV-2/Ad2-infected HeLa ...of 2) and harvested 24 h postinfection by preparation of nuclei and ...both compartments were analyzed by SDS-PAGE and Western blotting ... See full document
9
Herpes Simplex Virus Type 1/Adeno-Associated Virus rep+ Hybrid Amplicon Vector Improves the Stability of Transgene Expression in Human Cells by Site-Specific Integration
... the rep gene from ...clones, rep was detected in the cellular ...the rep gene we detected was intact and ...the rep gene was included in the integrated sequence without the backbone ...the ... See full document
13
Impact of the MRN Complex on Adeno-Associated Virus Integration and Replication during Coinfection with Herpes Simplex Virus 1
... Adeno-associated virus (AAV) is a helper-dependent parvovirus that requires coinfection with adenovirus (AdV) or herpes sim- plex virus 1 (HSV-1) to ...helper ... See full document
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Inhibition of Herpes Simplex Virus Type 1 Replication by Adeno-Associated Virus Rep Proteins Depends on Their Combined DNA-Binding and ATPase/Helicase Activities
... Rep78- induced DNA damage signaling, in that expression of Rep78 did not result in cell cycle arrest and activation of H2AX in ATM-null cell lines ...to replication stalling and single-strand breaks and is ... See full document
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Recombinant adeno-associated virus type 2 replication and packaging is entirely supported by a herpes simplex virus type 1 amplicon expressing Rep and Cap.
... of rep- and cap-inducible cell lines has been difficult, and the yield of rAAV produced from different clones is variable and does not exceed the efficiency of transfection methods (8, 9, ...of rep and cap ... See full document
10
Rep-Dependent Initiation of Adeno-Associated Virus Type 2 DNA Replication by a Herpes Simplex Virus Type 1 Replication Complex in a Reconstituted System
... Adeno-associated virus type 2 (AAV), a member of the parvovirus family, contains a single-stranded genome of 4,679 ...the replication (Rep) proteins and the other coding ... See full document
9
Adeno-Associated Virus Type 2 Rep68 Can Bind to Consensus Rep-Binding Sites on the Herpes Simplex Virus 1 Genome
... the replication of the HSV-1 replicon to some extent ...the Rep-Alexa Fluor 594 signals in the merged images of panels l to n and q to s in ...the Rep-LacI construct and the LacI-EYFP reporter ... See full document
9
Subcellular compartmentalization of adeno-associated virus type 2 assembly.
... adenovirus type 2 ...AAV-2 virus stocks, and recombinant VP2/3 containing empty virus- like particles prepared from baculovirus-infected SF9 cells ...AAV virus stocks ... See full document
12
Factors Affecting the Terminal Resolution Site Endonuclease, Helicase, and ATPase Activities of Adeno-Associated Virus Type 2 Rep Proteins
... higher-order Rep protein complexes on hairpin DNA in which some Rep molecules are associated indirectly with DNA via protein-pro- tein interactions which are weaker than the direct protein- DNA ...at ... See full document
10
Formation of herpes simplex virus type 1 replication compartments by transfection: requirements and localization to nuclear domain 10.
... for replication compartment formation by transfection. Replication of an origin-containing plasmid in a transient transfection assay occurs only in the presence of all seven essential replication ... See full document
10
DNase Induced After Infection of KB Cells by Herpes Simplex Virus Type 1 or Type 2 II. Characterization of an Associated Endonuclease Activity
... pMB9 DNA and PM2 DNA were reacted with 1.0 unit of HSV-2 DNase in the standard assay mixture but in the presence of 5 mM Mn2 each of the times indicated, and the native and denatured pro[r] ... See full document
9
Herpes simplex virus type 1-induced hydrocephalus in mice.
... When the cranial cavities of these mice were opened, hydrocephali had developed in the group of mice that received whole immune spleen cells and those treated with anti-immunoglobulin pl[r] ... See full document
10
The adeno-associated virus rep gene inhibits replication of an adeno-associated virus/simian virus 40 hybrid genome in cos-7 cells.
... Because the rep proteins is required for utilization of the TRs as origins of replication in helper virus-infected cells, it seems logical that the cis-active target sequences required f[r] ... See full document
8
Impact of Capsid Conformation and Rep-Capsid Interactions on Adeno-Associated Virus Type 2 Genome Packaging
... four Rep proteins except a weak reduction in the levels of Rep68 and Rep40 in V221W, N334W, and S338A/V221W mutants ...Table 1) could be observed for three mutants (V221W, N334W, and S338A/V221W ...small ... See full document
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