[PDF] Top 20 Identification of Less Common Streptococcus pneumoniae Serotypes by a Multiplex PCR Based Reverse Line Blot Hybridization Assay
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Identification of Less Common Streptococcus pneumoniae Serotypes by a Multiplex PCR Based Reverse Line Blot Hybridization Assay
... a multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay to identify 40 Strepto- coccus pneumoniae serotypes, including the 23 ... See full document
5
Reverse Line Blot Assay for Direct Identification of Seven Streptococcus agalactiae Major Surface Protein Antigen Genes
... a multiplex PCR-based reverse line blot hybridization assay (mPCR/RLB) to detect the genes encoding members of the family of variable surface-localized proteins of ... See full document
5
Multiplex PCR Based Reverse Line Blot Hybridization Assay To Identify 23 Streptococcus pneumoniae Polysaccharide Vaccine Serotypes
... 62.13 AF316642 12478CGA ACC ATT GAA ACT ATC ATC TG12456 19ASb wzy Serotype 19A 61.40 AF094575 9260TGT ATT TGC CCT TAT TAA TGT GC9282 19AAp wzy Serotype 19A 61.12 AF094575 9336TGC CAC TAA TAA TCA AAA GAT AAG C9312 19ASp ... See full document
5
Comparative study of bacteriological culture and real-time fluorescence quantitative PCR (RT-PCR) and multiplex PCR-based reverse line blot (mPCR/RLB) hybridization assay in the diagnosis of bacterial neonatal meningitis
... Molecular methods, including RT-PCR and mPCR/ RLB, do not depend on the presence of viable or grow- ing bacteria, and thus are suitable to the detection of pathogens that cannot be cultured readily by routine ... See full document
8
PCR Reverse Blot Hybridization Assay for Screening and Identification of Pathogens in Sepsis
... Klebsiella pneumoniae, Pseudomo- nas aeruginosa, Acinetobacter baumannii, Haemophilus influenzae, Citrobacter freundii, Streptococcus pneumoniae, ... See full document
7
Simultaneous Identification of 14 Genital Microorganisms in Urine by Use of a Multiplex PCR Based Reverse Line Blot Assay
... mPCR/RLB hybridization assay that permits the reliable, simultaneous detection of 14 known or potential urogenital pathogens, many of which are difficult to identify by other ...and less expensive ... See full document
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Comparison of Two Widely Used Human Papillomavirus Detection and Genotyping Methods, GP5+/6+ Based PCR Followed by Reverse Line Blot Hybridization and Multiplex Type Specific E7 Based PCR
... various PCR-based methods for HPV DNA detection and genotyping, of which one of the most commonly used in epidemiological studies is the GP5⫹/6⫹ primer set targeting conserved sequences within the L1 gene, ... See full document
8
Use of PCR and Reverse Line Blot Hybridization Assay for Rapid Simultaneous Detection and Serovar Identification of Chlamydia trachomatis
... evaluate multiplex and nested PCR-reverse line blot (RLB) hybridiza- tion assays for detection and serovar identification of Chlamydia ...in multiplex (both targets) and ... See full document
6
Simultaneously Typing Nine Serotypes of Enteroviruses Associated with Hand, Foot, and Mouth Disease by a GeXP Analyzer Based Multiplex Reverse Transcription PCR Assay
... The identification and serotyping of enteroviruses have been based on the time-consuming and labor-intensive procedures of viral isolation in cell culture and neutralization with mixed hyper- immune equine ... See full document
6
Identification of 20 Common Human Enterovirus Serotypes by Use of a Reverse Transcription PCR Based Reverse Line Blot Hybridization Assay
... (HEV) serotypes can also be classified into four species, HEV-A to -D, based on phylogenetic analysis of multiple gene ...on reverse transcription-PCR (RT-PCR) amplification and ... See full document
7
Rifampin Isoniazid Oligonucleotide Typing: an Alternative Format for Rapid Detection of Multidrug Resistant Mycobacterium tuberculosis
... A reverse line blot DNA hybridization format for rapid detection of multidrug-resistant tuberculosis was ...was based on the same amplification/reverse hybridization ... See full document
6
Multiplex PCR Assay for Detection of Streptococcus suis Species and Serotypes 2 and 1/2 in Tonsils of Live and Dead Pigs
... Serological analysis. All sera were tested with an indirect enzyme-linked immunosorbent assay (ELISA) using a protein extract from sonicated bacteria as antigen. This ELISA test was not attempted to be used as a ... See full document
7
Rapid Detection and Identification of Human Adenovirus Species by Adenoplex, a Multiplex PCR Enzyme Hybridization Assay
... Adenoplex assay has advantages over several of the PCR-based assays recently described in the ...specific multiplex assay they had poor amplification of sero- types 11, 21, 34, and 35 ... See full document
5
Molecular Diagnosis of Old World Cutaneous Leishmaniasis and Species Identification by Use of a Reverse Line Blot Hybridization Assay
... Reverse line blot hybridization assays (RLB) have been used for the rapid diagnosis and genotyping of many ...ITS1 PCR primers and the ...ITS1 PCR and can detect ...ITS1 ... See full document
8
Simultaneous Detection of Streptococcus pneumoniae, S mitis, and S oralis by a Novel Multiplex PCR Assay Targeting the gyrB Gene
... 1,548-bp PCR products, respectively, showing positive ...of PCR products from the 87 strains of other closely related streptococci or Gram-positive cocci were detected (data not ...negative based on ... See full document
6
Multiplex PCR Based Method for Identification of Common Clinical Serotypes of Salmonella enterica subsp enterica
... with PCR-based serotyping is the inability to discriminate false positives, ...rare serotypes that create the same amplicon pat- tern as the more common ...these multiplex PCR ... See full document
8
Serotyping of Streptococcus pneumoniae isolated from Tehran by Multiplex PCR: Are serotypes of clinical and carrier isolates identical?
... S. pneumoniae carriage rate. A study of 1300 healthy children less than ten years-old in Tehran, Iran showed pneumococcal nasopharyngeal carriage rate of ...S. pneumoniae in Iranian healthy ... See full document
7
Rapid Diagnosis of Extensively Drug Resistant Tuberculosis by Use of a Reverse Line Blot Hybridization Assay
... The reverse line blot hybridization (RLBH) assay (11, 24) is a probe-based method where multiple oligonucleotide probes carrying the mutant sequence and wild-type sequence are ... See full document
6
Simultaneous Detection and Identification of Candida, Aspergillus, and Cryptococcus Species by Reverse Line Blot Hybridization
... Our assay extends this to 17 yeast species and five Aspergillus ...to assay up to 43 specimens in a single run and for the mem- brane to be reused up to nine times without loss of ...present assay, ... See full document
5
Proposal of serovars 17 and 18 of Actinobacillus pleuropneumoniae based on serological and genotypic analysis
... Weinert, L.A., Chaudhuri, R.R., Wang, J., Peters, S.E., Corander, J., Jombart, T., Baig, A., Howell, K.J., Vehkala, M., Välimäki, N., Harris, D., Chieu, T.T.B., Van Vinh Chau, N., Campbell, J., Schultsz, C., Parkhill, ... See full document
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