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Identification of the E5 open reading frame of human papillomavirus type 16.
... 1071 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Sequencing of the E5 open reading frame ORF of human papillomavirus type 16 revealed an additional nucleotide, a th[r] ... See full document
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Identification of a G2 Arrest Domain in the E1∧E4 Protein of Human Papillomavirus Type 16
... Coleman, and J. Doorbar, Abstr. 19th Int. Papillomavirus Conf., abstr. P-21, 2001), in productive infections it is an abun- dant protein that is expressed in the upper layers of the epi- thelium. 16E1 ∧ E4 is ... See full document
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Identification of a differentiation-inducible promoter in the E7 open reading frame of human papillomavirus type 16 (HPV-16) in raft cultures of a new cell line containing high copy numbers of episomal HPV-16 DNA.
... of open reading frames (ORFs) E6 and E7 is spe- cifically retained and has been shown to be necessary for the growth potential of carcinoma cell lines (2, 42, 47, 49, 50, ...HPV- 16-positive cell ... See full document
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Identification of a transforming gene of human papillomavirus type 16.
... Monolayers of a G418-resistant population of NIH 3T3 cells 5 x 105 per 100-mm plate each mock-transfected a, or transfected with pSV2-neo b, pSVHPV16-M c, or cDNA cl-61 dl-5 d were incub[r] ... See full document
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Identification of Human Papillomavirus Type 16 L1 Surface Loops Required for Neutralization by Human Sera
... VLP type was normalized to that of HPV16 wild-type VLPs after subtracting the HPV31 wild-type VLP optical density ...the 16:HI VLPs could not adsorb antibodies from any sera tested and it was ... See full document
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Identification of T- and B-cell epitopes of the E7 protein of human papillomavirus type 16.
... Two of the B-cell epitopes were recognized by antisera from mice with three different murine H-2 haplotypes k, d, and s immunized with two different E7 fusion proteins and from Fischer r[r] ... See full document
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Expression of human papillomavirus type 16 E6 E7 open reading frame varies quantitatively in biopsy tissue from different grades of cervical intraepithelial neoplasia
... the human papillomavirus type 16 E6-E7 open reading frame are essential for transformation of the host ...The identification of distinct patterns of expression in ... See full document
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The Human Papillomavirus Type 16 E5 Oncoprotein Inhibits Epidermal Growth Factor Trafficking Independently of Endosome Acidification
... the identification of specific proteins that regulate endocytic trafficking that may be targeted by 16E5; however, several potential mechanisms for the 16E5-dependent trafficking de- fect can already be ruled ... See full document
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The E5 oncoprotein of human papillomavirus type 16 inhibits the acidification of endosomes in human keratinocytes.
... basal human kera- tinocytes and propagate in the differentiating layers of the epithelium ...and E5 proteins possess onco- genic activities that contribute to the pathogenesis associated with HPV infection ... See full document
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Specific Inactivation of Inhibitory Sequences in the 5′ End of the Human Papillomavirus Type 16 L1 Open Reading Frame Results in Production of High Levels of L1 Protein in Human Epithelial Cells
... Zhou et al. observed that the BPV-1 L1 gene contains rare translational codons (57). They therefore replaced the rare codons with more commonly used codons and analyzed expres- sion levels of a wild-type and a ... See full document
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Human antibodies react with an epitope of the human papillomavirus type 6b L1 open reading frame which is distinct from the type-common epitope.
... Human sera which reacted with the HPV6b Li ORF-encoded fusion proteins and rabbit anti-BPV1 were tested for reactivity to Li fusion proteins encoded by HPVla, HPV11, HPV16, and HPV18 DNA[r] ... See full document
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Human Papillomavirus Type 16 E7 DNA Vaccine: Mutation in the Open Reading Frame of E7 Enhances Specific Cytotoxic T-Lymphocyte Induction and Antitumor Activity
... Our data indicate that mutation in the zinc-binding motifs near the C terminus of E7 leads to rapid degradation of the protein. This finding is based on the fact that the mutant E7 can be better detected in the presence ... See full document
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Analyses of variant human papillomavirus type-16 E5 proteins for their ability to induce mitogenesis of murine fibroblasts
... binant human EGF (20 μg/l: Boehringer-Mannheim ...each reading after separation of single intact nuclei from debris and cell-clumps by gating on an FL2 area/width ... See full document
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Human papillomavirus type 16 transformation of primary human embryonic fibroblasts requires expression of open reading frames E6 and E7.
... Since extension of the life span is characteristic of the human fibroblasts transformed by the whole HPV-16 genome 22, we concluded that transfection with HPV-16 E6E7 DNA fragments induc[r] ... See full document
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Identification and Prioritisation of Variants in the Short Open-Reading Frame Regions of the Human Genome
... variant type is present at the same genomic location in each VCF does not account for the problem that there are multiple ways of representing the same variant; one multinucleotide polymorphism in the first VCF ... See full document
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E5 open reading frame of bovine papillomavirus type 1 encodes a transforming gene.
... In C127 cells, pHLB1 normally induced only about one-half as many foci as the full-length BPV clone, Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest MATERIALS AND METHO[r] ... See full document
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The E7 Open Reading Frame Acts in cis and in trans To Mediate Differentiation-Dependent Activities in the Human Papillomavirus Type 16 Life Cycle
... The ultimate test of the viral mutants is the ability to gen- erate virions. Interestingly, we observed that cell lines with episomal HPV16 were able to synthesize virions despite the lack of significant ... See full document
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Accumulation of RNA homologous to human papillomavirus type 16 open reading frames in genital precancers.
... The early region contains ORFs expressed in human cervical cancers and cell lines derived from them as well as sequences which correspond to the transforming genes from BPV 12, 28-30, 33[r] ... See full document
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Regulation of human papillomavirus type 11 enhancer and E6 promoter by activating and repressing proteins from the E2 open reading frame: functional and biochemical studies.
... In this paper, we report the expression of HPV-11 E2-C, a protein consisting mainly of the carboxy-terminal 45% of the E2 protein, and the regulation of HPV-11 enhancer-SV40 early promot[r] ... See full document
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Translation of the human papillomavirus type 16 E7 oncoprotein from bicistronic mRNA is independent of splicing events within the E6 open reading frame.
... Second, ribosomal 40S subunits could engage the E7 AUG by a scanning-independent mechanism such as internal entry. However, an internal entry site should be insensitive to changes at the 5 9 end of the molecule, yet we ... See full document
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