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[PDF] Top 20 Inactivation of the RNase Activity of Glycoprotein Erns of Classical Swine Fever Virus Results in a Cytopathogenic Virus

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Inactivation of the RNase Activity of Glycoprotein Erns of Classical Swine Fever Virus Results in a Cytopathogenic Virus

Inactivation of the RNase Activity of Glycoprotein Erns of Classical Swine Fever Virus Results in a Cytopathogenic Virus

... enzyme activity of NS3 could play a role in the induction of cell ...noncytopathogenic virus and that NS3 could inhibit this function of NS2-3 in cells infected with cyto- pathogenic ...with ... See full document

7

Mutations Abrogating the RNase Activity in Glycoprotein Erns of the Pestivirus Classical Swine Fever Virus  Lead to Virus Attenuation

Mutations Abrogating the RNase Activity in Glycoprotein Erns of the Pestivirus Classical Swine Fever Virus Lead to Virus Attenuation

... the RNase but is also essential for other im- portant functions of this ...on virus propagation in tissue ...a virus was recovered that grew as well as the wild-type ...the RNase ... See full document

12

RNase of classical swine fever virus: biochemical characterization and inhibition by virus-neutralizing monoclonal antibodies.

RNase of classical swine fever virus: biochemical characterization and inhibition by virus-neutralizing monoclonal antibodies.

... E0 RNase activity (data not ...enzymatic activity of E0. These results also show that the tertiary structure of E0 is very rigid and that the RNase is enzymatically active over a wide ... See full document

7

Porcine Viperin protein inhibits the replication of classical swine fever virus (CSFV) in vitro

Porcine Viperin protein inhibits the replication of classical swine fever virus (CSFV) in vitro

... antiviral activity to a broad range of viruses and modulating innate immune signaling ...viral activity against a range of DNA and RNA viruses, including HCMV, HCV, West Nile virus (WNV), Dengue ... See full document

11

Interaction of Classical Swine Fever Virus with Membrane-Associated Heparan Sulfate: Role for Virus Replication In Vivo and Virulence

Interaction of Classical Swine Fever Virus with Membrane-Associated Heparan Sulfate: Role for Virus Replication In Vivo and Virulence

... vivo virus to attach to the cell surface by interaction with HS is a result of its specific surface ...of virus produced in SK6 ...the results of these tests are presented for pigs 4071 (S-ST) and ... See full document

11

Substitution of Specific Cysteine Residues in the E1 Glycoprotein of Classical Swine Fever Virus Strain Brescia Affects Formation of E1-E2 Heterodimers and Alters Virulence in Swine

Substitution of Specific Cysteine Residues in the E1 Glycoprotein of Classical Swine Fever Virus Strain Brescia Affects Formation of E1-E2 Heterodimers and Alters Virulence in Swine

... DNA sequencing and analysis. Full-length clones were completely sequenced with CSFV-specific primers by the dideoxynucleotide chain-termination method (16). In vitro rescued viruses and viruses recovered from infected ... See full document

9

Enzyme-Linked Immunosorbent Assay Based on a Chimeric Antigen Bearing Antigenic Regions of Structural Proteins Erns and E2 for Serodiagnosis of Classical Swine Fever Virus Infection

Enzyme-Linked Immunosorbent Assay Based on a Chimeric Antigen Bearing Antigenic Regions of Structural Proteins Erns and E2 for Serodiagnosis of Classical Swine Fever Virus Infection

... 3. Chien, D. Y., Q. L. Choo, A. Tabrizi, C. Kuo, J. McFarland, K. Berger, C. Lee, J. R. Shuster, T. Nguyen, D. L. Moyer, M. Tong, S. Furuta, M. Omata, G. Tegtimeier, H. Alter, E. Schiff, L. Jeffers, M. Houghton, and G. ... See full document

5

Classical swine fever virus: independent induction of protective immunity by two structural glycoproteins.

Classical swine fever virus: independent induction of protective immunity by two structural glycoproteins.

... A basic requirement of vaccines against CSF is the induction of protective immunity against a broad range of virus strains. Challenge infections in previous protection experiments with recombinant E2 were carried ... See full document

8

Pathogenesis of classical swine fever: B-lymphocyte deficiency caused by hog cholera virus.

Pathogenesis of classical swine fever: B-lymphocyte deficiency caused by hog cholera virus.

... Already at the onset of disease, viral replication in lymphoid tissues demarcates the germinal centers, and the viral genome remains localized to that site as the disease progresses even[r] ... See full document

5

Characterization of Essential Domains and Plasticity of the Classical Swine Fever Virus Core Protein

Characterization of Essential Domains and Plasticity of the Classical Swine Fever Virus Core Protein

... incorporation of YFP Core into the virion was evident from Western blot analysis, inspection of the sequence of the Core region from rescued viruses by RT-PCR revealed that no ge- netic change had occurred in either the ... See full document

9

Proteomic analysis of swine serum following highly virulent classical swine fever virus infection

Proteomic analysis of swine serum following highly virulent classical swine fever virus infection

... analysis, the peptide powders were re-dissolved in 5 μl 0.1% TFA. One μl of supernatant was spotted on anchorChip targets (Bruker Daltonics), and then mixed with 0.5 μl matrix solution (10 mg/ml a-cyano-4-hydro- ... See full document

7

In vitroinfection with classical swine fever virus inhibits the transcription of immune response genes

In vitroinfection with classical swine fever virus inhibits the transcription of immune response genes

... diarrhea virus (BVDV) can estab- lish persistent infection by inhibiting the induction of type I interferon in vitro and in vivo ...HIV-1 virus infects CD4 + T cells, and the depletion of these cells is a ... See full document

11

Biological and molecular characterization of classical swine fever challenge virus from Mongolia

Biological and molecular characterization of classical swine fever challenge virus from Mongolia

... омог сэргээхээс 2 хоногийн өмнө урьдчилан туршилтын байранд дасгасан болно. Үүний дараа шалгалтын омгийг торойн хүзүүг гурав хуваасны дундах хэсгийн булчинд шалгалтын “Унгар” омгоос тарьж халдаав. Омог сэргээлтийн явцад ... See full document

7

Experimental infection of Bama miniature pigs with a highly virulent classical swine fever virus

Experimental infection of Bama miniature pigs with a highly virulent classical swine fever virus

... the virus, thus dose effects were not revealed; 3) The differences are not statistically significantly different in most ...the results of the present study revealed obvious differences between domestic ... See full document

7

Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs

Dynamic distribution and tissue tropism of classical swine fever virus in experimentally infected pigs

... of virus load and little is known about the virus load in relation to dis- ease ...via virus isola- tion, RT-PCR and real time PCR[6] was somewhat lim- ited, only demonstrating the presence or ... See full document

10

Hemoglobin Subunit Beta Interacts with the Capsid Protein and Antagonizes the Growth of Classical Swine Fever Virus

Hemoglobin Subunit Beta Interacts with the Capsid Protein and Antagonizes the Growth of Classical Swine Fever Virus

... the swine RIG-I gene (Fig. 9A). The results showed that knockdown of RIG-I increased CSFV growth and replication in PK-15 cells ...The results indi- cated that SK6 cells were more efficiently ... See full document

11

Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus

Thioredoxin 2 Is a Novel E2-Interacting Protein That Inhibits the Replication of Classical Swine Fever Virus

... mediate virus attachment ...cells. Virus titers in supernatant collected at 48 and 72 hpi were determined and expressed as 50% tissue culture infective doses (TCID 50 ... See full document

15

Crystal Structure of Classical Swine Fever Virus NS5B Reveals a Novel N-Terminal Domain

Crystal Structure of Classical Swine Fever Virus NS5B Reveals a Novel N-Terminal Domain

... polymerase activity using an in vitro RNA synthesis assay ...previous results (data not shown) indicated that CSFV NS5B preferred PE46-derived RNA synthesis, while little detectable RNA product was ... See full document

13

Important roles of C terminal residues in degradation of capsid protein of classical swine fever virus

Important roles of C terminal residues in degradation of capsid protein of classical swine fever virus

... proteasome. Results showed that MG132 increased pro- tein level of cleaved C-EGFP in PK-15 and 3-MA had no effect on protein level of cleaved C-EGFP ...blot results showed that cleaved form of C-EGFP ... See full document

10

Efficacy of a live attenuated vaccine in classical swine fever virus postnatally persistently infected pigs

Efficacy of a live attenuated vaccine in classical swine fever virus postnatally persistently infected pigs

... for virus replication [36], as is the case for the CSFV Catalonia strain that induced the persistence in these ...vaccine virus did not follow this logic, given the lack of viral detec- tion in samples ... See full document

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