Top PDF Inhibition of Cellular DNA Synthesis by the Human Cytomegalovirus IE86 Protein Is Necessary for Efficient Virus Replication
Inhibition of Cellular DNA Synthesis by the Human Cytomegalovirus IE86 Protein Is Necessary for Efficient Virus Replication
... mutant IE86 protein has yielded important information about the function, and possibly the structure, of the IE86 ...mutant IE86 protein maintains the ability to auto- regulate ...
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The 5′ Untranslated Region of the Major Immediate Early mRNA Is Necessary for Efficient Human Cytomegalovirus Replication
... The human cytomegalovirus (HCMV) immediate early 1 (IE1) and IE2 proteins are critical regulators of virus ...IE2 protein expression is thus a central event in the outcome of HCMV ...was ...
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Identification of an Arginine-Rich Motif in Human Papillomavirus Type 1 E1^E4 Protein Necessary for E4-Mediated Inhibition of Cellular DNA Synthesis In Vitro and in Cells
... cell DNA synthesis is blocked during the Epstein-Barr virus (EBV) lytic infection cycle, during which there is high- level amplification of the EBV genome ...block cellular DNA ...
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Asparagine Deprivation Causes a Reversible Inhibition of Human Cytomegalovirus Acute Virus Replication
... for human cytomegalovirus (HCMV), consisting of 6,881 ...of cellular processes, including the mediator complex, pro- teasome function, and mRNA ...HCMV replication which leads to an early ...
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Inhibition of Cell Division by the Human Cytomegalovirus IE86 Protein: Role of the p53 Pathway or Cyclin-Dependent Kinase 1/Cyclin B1
... one cellular protein regulating cellular proliferation, we also tested HCT116 cells and isogenic mutant cells that lack either p53 or p21 through specific knockout of these genes by homol- ogous ...
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pUL69 of Human Cytomegalovirus Recruits the Cellular Protein Arginine Methyltransferase 6 via a Domain That Is Crucial for mRNA Export and Efficient Viral Replication
... in-frame fusion of a FLAG tag as well as the simian virus 40 (SV40) T antigen nuclear localization signal (NLS). C-terminal deletion constructs of the R/A substitution mutants that lack the amino acids 147 to 744 ...
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Human Cytomegalovirus Early Protein pUL21a Promotes Efficient Viral DNA Synthesis and the Late Accumulation of Immediate-Early Transcripts
... viral DNA synthesis reduces the late accumu- lation of IE transcripts in wild-type and UL21a deletion virus- infected cells to equivalent ...viral DNA synthesis during HCMV infec- tion, ...
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Repression of HMGA2 Gene Expression by Human Cytomegalovirus Involves the IE2 86-Kilodalton Protein and Is Necessary for Efficient Viral Replication and Inhibition of Cyclin A Transcription
... Human cytomegalovirus (HCMV) infection results in dysregulation of several cell cycle genes, including inhibition of cyclin A ...the inhibition of cyclin A expression and impacts on the ...
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The Autoregulatory and Transactivating Functions of the Human Cytomegalovirus IE86 Protein Use Independent Mechanisms for Promoter Binding
... mutant IE86 BAC DNA was detected at day 1. Relative to Towne (WT IE86) BAC DNA input at day 1, BAC DNA containing WT and Rev-PY IE86 increased approximately 12- fold by day 13 ...
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The Ubiquitin-Proteasome System Is Necessary for Efficient Replication of Human Astrovirus
... proteasome inhibition is not related to the induction of autophagy or ...to virus release (10, 11, 40), but at early times during infection, apoptosis could reduce viral ...the virus yield were ...
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Human Cytomegalovirus Can Procure Deoxyribonucleotides for Viral DNA Replication in the Absence of Retinoblastoma Protein Phosphorylation
... Viral DNA replication requires deoxyribonucleotide triphosphates ...novo synthesis. Nucleotide synthesis utilizes the activity of a series of nucleotide-biosynthetic enzymes (NBEs) whose ...
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The Flexible Loop of the Human Cytomegalovirus DNA Polymerase Processivity Factor ppUL44 Is Required for Efficient DNA Binding and Replication in Cells
... to cellular dsDNA when expressed even in the absence of viral DNA is not surprising, since ppUL44 appears to be able to bind to dsDNA without any sequence specificity ...oriLyt-dependent DNA ...
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Inhibition of human immunodeficiency virus replication by the herpes simplex virus virion host shutoff protein.
... simplex virus (HSV) virion host shutoff gene (vhs) encodes a protein which nonspecifically accelerates the degradation of mRNA molecules, leading to inhibition of protein ...critical ...
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Nucleolin Associates with the Human Cytomegalovirus DNA Polymerase Accessory Subunit UL44 and Is Necessary for Efficient Viral Replication
... viral DNA synthesis is required for the association with ...FLAG44 virus. (A) Replication of AD169rv and FLAG44 ...and virus supernatant was harvested at the indicated time points. The ...
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Functional Properties of the Human Cytomegalovirus IE86 Protein Required for Transcriptional Regulation and Virus Replication
... UL54 DNA polymerase (17, 23, 41, 51, 55, ...by IE86 is thought to be mediated by direct binding of IE86 to DNA and/or protein interactions with cellular and viral transcription ...
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Human Cytomegalovirus Infection Leads to Accumulation of Geminin and Inhibition of the Licensing of Cellular DNA Replication
... Human cytomegalovirus (HCMV), an ubiquitous betaher- pesvirus, is the leading viral cause of birth defects and poses a serious health threat to immunocompromised individuals ...the virus and the host ...
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Interactome Analysis of the Influenza A Virus Transcription/Replication Machinery Identifies Protein Phosphatase 6 as a Cellular Factor Required for Efficient Virus Replication
... the cellular interactome of the viral transcription/replication machin- ...A virus encoding a Strep-tag at the C terminus of the PB2 subunit of the viral RdRP to isolate protein complexes ...
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Role of Homodimerization of Human Cytomegalovirus DNA Polymerase Accessory Protein UL44 in Origin-Dependent DNA Replication in Cells
... oriLyt-dependent DNA replication was tested by the means of a cotransfection-replication assay as described previously (22, ...oriLyt DNA replication origin (5), a plasmid expressing ...
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unit-1-dna-replication-and-protein-synthesis
... Messenger RNA (mRNA) and transfer RNA (tRNA) are important nucleic acids involved in the process of protein synthesis. (a) Describe how a molecule of mRNA is made during transcription[r] ...
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Human Parvovirus B19 Utilizes Cellular DNA Replication Machinery for Viral DNA Replication
... for protein-protein interacting proteins (PPI Hub ...visualize protein- protein interacting networks and to integrate gene expression ...
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