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[PDF] Top 20 Initiation of protein synthesis by internal entry of ribosomes into the 5' nontranslated region of encephalomyocarditis virus RNA in vivo.

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Initiation of protein synthesis by internal entry of ribosomes into the 5' nontranslated region of encephalomyocarditis virus RNA in vivo.

Initiation of protein synthesis by internal entry of ribosomes into the 5' nontranslated region of encephalomyocarditis virus RNA in vivo.

... Symbols: angled open box, cap-binding protein CBP complex eIF-4F and other initiation factors; shaded oval, a 40S ribosomal subunit; hatched circle, hypothetical factors which recognizes[r] ... See full document

10

Hepatitis C Virus-Encoded Enzymatic Activities and Conserved RNA Elements in the 3′ Nontranslated Region Are Essential for Virus Replication In Vivo

Hepatitis C Virus-Encoded Enzymatic Activities and Conserved RNA Elements in the 3′ Nontranslated Region Are Essential for Virus Replication In Vivo

... HCV RNA element examined in this study was the conserved portion of the 3⬘ ...genome RNA terminated with poly(A) (20) or poly(U) (for examples, see references 21, 30, and ...short region that varies ... See full document

6

In Vivo and In Vitro Identification of Structural and Sequence Elements of the Human Parechovirus 5′ Untranslated Region Required for Internal Initiation

In Vivo and In Vitro Identification of Structural and Sequence Elements of the Human Parechovirus 5′ Untranslated Region Required for Internal Initiation

... sense RNA viruses, which includes a number of important human and animal ...cell protein synthesis (8, ...a virus particle with only three capsid proteins rather than four; lack of ... See full document

9

Cell Type-Specific Enhancement of Hepatitis C Virus Internal Ribosome Entry Site-Directed Translation due to 5′ Nontranslated Region Substitutions Selected during Passage of Virus in Lymphoblastoid Cells

Cell Type-Specific Enhancement of Hepatitis C Virus Internal Ribosome Entry Site-Directed Translation due to 5′ Nontranslated Region Substitutions Selected during Passage of Virus in Lymphoblastoid Cells

... modified 5⬘NTR sequences in cultured lymphoid cells (23), coupled with the demonstration of identical 5⬘NTR sequences in PBMC collected from infected chimpanzees (30), suggests that virus with these ... See full document

8

The sequence element of the internal ribosome entry site and a 25-kilodalton cellular protein contribute to efficient internal initiation of translation of hepatitis C virus RNA.

The sequence element of the internal ribosome entry site and a 25-kilodalton cellular protein contribute to efficient internal initiation of translation of hepatitis C virus RNA.

... C virus (HCV) RNA is initiated by internal entry of ribosomes into the 5 * noncoding region ...the 5 * NCR called the internal ribosome entry site ... See full document

5

The 5' nontranslated region of potato virus X RNA affects both genomic and subgenomic RNA synthesis.

The 5' nontranslated region of potato virus X RNA affects both genomic and subgenomic RNA synthesis.

... plus-strand- RNA production. Given that minus-strand RNA did accumu- late in protoplasts inoculated with mutant transcripts, these data suggest that PVX subgenomic RNA cannot be generated by ... See full document

8

Natural Variation in Translational Activities of the 5′ Nontranslated RNAs of Hepatitis C Virus Genotypes 1a and 1b: Evidence for a Long-Range RNA-RNA Interaction outside of the Internal Ribosomal Entry Site

Natural Variation in Translational Activities of the 5′ Nontranslated RNAs of Hepatitis C Virus Genotypes 1a and 1b: Evidence for a Long-Range RNA-RNA Interaction outside of the Internal Ribosomal Entry Site

... in vivo (see below), we constructed plasmids (pN-CLuc and pH-CLuc) in which sequence encoding the re- porter protein, firefly luciferase, was fused in frame at nt 408 of the HCV sequence in pN-CDE1 and ... See full document

11

Internal entry of ribosomes is directed by the 5' noncoding region of classical swine fever virus and is dependent on the presence of an RNA pseudoknot upstream of the initiation codon.

Internal entry of ribosomes is directed by the 5' noncoding region of classical swine fever virus and is dependent on the presence of an RNA pseudoknot upstream of the initiation codon.

... 373-nucleotide-long 5 * nontranslated region (NTR) of the classical swine fever virus (CSFV) genome as intercistronic spacer were used to show the presence of an internal ribosome ... See full document

7

A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation.

A segment of the 5' nontranslated region of encephalomyocarditis virus RNA directs internal entry of ribosomes during in vitro translation.

... The effect of the poliovirus 5'NTR on in vitro translation was compared with that of the 5'NTR of encephalomyocarditis virus by the use of synthetic mRNAs, micrococcal nuclease-treated H[r] ... See full document

8

In vitro characterization of an internal ribosomal entry site (IRES) present within the 5' nontranslated region of hepatitis A virus RNA: comparison with the IRES of encephalomyocarditis virus.

In vitro characterization of an internal ribosomal entry site (IRES) present within the 5' nontranslated region of hepatitis A virus RNA: comparison with the IRES of encephalomyocarditis virus.

... Although AUG-12 is the preferred initiation codon when the full-length 5'NTR is present, either Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest DISCUSSION Earlier work, [r] ... See full document

9

Intracellular modifications induced by poliovirus reduce the requirement for structural motifs in the 5' noncoding region of the genome involved in internal initiation of protein synthesis.

Intracellular modifications induced by poliovirus reduce the requirement for structural motifs in the 5' noncoding region of the genome involved in internal initiation of protein synthesis.

... The analysis of deletion mutants in cells cotransfected with a complete poliovirus cDNA unexpectedly revealed that under conditions in which virus proteins were being synthesized, consid[r] ... See full document

7

Mutations within the 5' nontranslated region of hepatitis A virus RNA which enhance replication in BS-C-1 cells.

Mutations within the 5' nontranslated region of hepatitis A virus RNA which enhance replication in BS-C-1 cells.

... Analysis of the growth properties of viruses rescued from these chimeras indicates that mutations at bases 152 and/or 203 to 207 of the 5'NTR strongly enhance replication competence in c[r] ... See full document

8

Temperature-sensitive hepatitis A virus mutants with deletions downstream of the first pyrimidine-rich tract of the 5' nontranslated RNA are impaired in RNA synthesis.

Temperature-sensitive hepatitis A virus mutants with deletions downstream of the first pyrimidine-rich tract of the 5' nontranslated RNA are impaired in RNA synthesis.

... 2-h virus adsorption ...intracellular virus titers by RIFA (see Mate- rials and ...intracellular virus yields present at 90 h ...viral entry and uncoating should be largely completed in a ... See full document

9

Internal Initiation Is Responsible for Synthesis of Wuhan Nodavirus Subgenomic RNA

Internal Initiation Is Responsible for Synthesis of Wuhan Nodavirus Subgenomic RNA

... encodes protein B2, which is a potent RNA- silencing ...sgRNA3 synthesis is still poorly ...the synthesis of WhNV sgRNA3 is counterregulated by the replication of WhNV genomic RNA2, which ... See full document

12

In vivo transcription of the 5'-terminal extracistronic region of vesicular stomatitis virus RNA.

In vivo transcription of the 5'-terminal extracistronic region of vesicular stomatitis virus RNA.

... After complete digestion with RNase T1 followed by the inactivation of the RNase with proteinase K, the resistant duplexes were heat denatured and separated on a polyacrylamide gel next [r] ... See full document

7

Revertant analysis of J-K mutations in the encephalomyocarditis virus internal ribosomal entry site detects an altered leader protein.

Revertant analysis of J-K mutations in the encephalomyocarditis virus internal ribosomal entry site detects an altered leader protein.

... (RPS 5 5; relative translation of 90%) was not as robust as the direct reversions but was still among the most active of tested ...(RPS 5 10) were mapped to IRES posi- tion 768, near the base of the ... See full document

6

Regulation of Viral RNA Synthesis by the V Protein of Parainfluenza Virus 5

Regulation of Viral RNA Synthesis by the V Protein of Parainfluenza Virus 5

... parainfluenza virus 3 (HPIV3), and MeV and CAT ...Nipah virus and the Nipah virus chloramphenicol acetyltransferase (CAT) minigenome have been previously published (35, 36), and details about the ... See full document

13

Structural Elements in the 5′-Untranslated Region of Giardiavirus Transcript Essential for Internal Ribosome Entry Site-Mediated Translation Initiation

Structural Elements in the 5′-Untranslated Region of Giardiavirus Transcript Essential for Internal Ribosome Entry Site-Mediated Translation Initiation

... Downstream of the pseudoknot structure, a predicted stem- loop U4a (nt U204 to A219) with a short (4-bp) stem and a large (8-nt) loop (Fig. 1), was subjected to chemical probing and enzyme digestion. The results showed ... See full document

13

Rearrangements in the 5′ Nontranslated Region and Phylogenetic Analyses of Cucumber Mosaic Virus RNA 3 Indicate Radial Evolution of Three Subgroups

Rearrangements in the 5′ Nontranslated Region and Phylogenetic Analyses of Cucumber Mosaic Virus RNA 3 Indicate Radial Evolution of Three Subgroups

... 5ⴕ NTR and phylogenetic analyses. Nucleotide sequences of the 5⬘ NTR or of the CP gene were initially aligned with the Pileup program of the Wisconsin Package, version 9.0 (Genetics Computer Group, Madison, Wis.). ... See full document

7

Structural Determinant of Human La Protein Critical for Internal Initiation of Translation of Hepatitis C Virus RNA

Structural Determinant of Human La Protein Critical for Internal Initiation of Translation of Hepatitis C Virus RNA

... monocistronic RNA was translated in RRL in the absence or presence of increasing concentrations (15, 30, and 60 ␮ M) of either the wt LaR2C-N7 or the mutant mLaR2C-N7-4 ...luciferase RNA was translated in ... See full document

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