[PDF] Top 20 Interlaboratory drug susceptibility testing of Mycobacterium tuberculosis by a radiometric procedure and two conventional methods

... A total of 224 recent isolates of Mycobacterium tuberculosis from 163 patients selected to have multidrug resistance were tested against streptomycin SM, isoiiazid, rifampin, and ethambu[r] ... See full document

... antimicrobial susceptibility testing of Mycobacterium tuberculosis was evaluated on 78 clinical ...repeat testing with all the four drugs. With susceptibility testing of ... See full document

... broth-based methods with a shortened incubation time were introduced into clinical laboratories to fulfill CDC ...of susceptibility to first- line ...the radiometric system and are being evaluated ... See full document

... persistent Mycobacterium tuberculosis than any other conventional anti-TB drug ...reliable testing of the sus- ceptibility of M. tuberculosis to PZA in vitro is challenging since ... See full document

... Drug susceptibility testing of PZA was performed through the automated BACTEC MGIT 960 system (BD Diagnostic Systems, NJ, USA) ...[14]. Mycobacterium tuberculosis H37Rv and ... See full document

... Fluorophages are a type of “reporter phage” that inject their DNA specifically into mycobacteria (24). Fluorescence is pro- duced by expression of a fluorescent reporter gene (11) cloned into the phage. Each ... See full document

... however, they require heavy equipment and are still con- strained by the cost of the machines. The manual MGIT sys- tem has been reported to be a sensitive and rapid method for DST to first-line drugs (19, 23), but ... See full document

... the drug susceptibility testing of typical mycobacterium tuberculosis directly from versa trek mycobottles with plant based isolates obtained from leaf of Wrightia tomentosa and thereto ... See full document

... Inoculation and incubation of the DST plates. The Mycobacterium isolates to be tested are suspended by adding a small loop of bacteria in 40 ml distilled water. To ensure biosafety, the bacteria are suspended in ... See full document

... Radiometric antimicrobial susceptibility tests, if performed by using pure culture recovered from a conventional solid medium, required approximately 5 days with 98.6% agreement with the[r] ... See full document

... For susceptibility testing using the Versa Trek ...a drug based on the following formula: No growth of myco bacterium species with the specific anti-mycobacterial drugs for more than 3 days, after ... See full document

... of drug sus- ceptibility are crucial to optimize treatment and prevent trans- ...for Mycobacterium tu- berculosis drug susceptibility testing is the proportion method, either on solid ... See full document

... the drug-free control suspensions to differentiate them from drug-treated ...between drug-free suspensions of mycobacteria and those treated with antimycobacterial ... See full document

... M. tuberculosis grows faster in liquid media than traditional solid media and also allows direct, simultaneous drug susceptibility testing by culturing the bacillus with first- and second-line ... See full document

... of drug containing and drug-free ...on drug containing medium with the number of colonies on drug free ...on drug free was between fifty and one hundred and fifty so that the number of ... See full document

... laboratory, testing of mycobacteria for drug susceptibility is substantially different from the general testing procedures used in ...single drug concentration, termed the critical ... See full document

... The following characteristics were determined for each test: sensitivity, the capacity of the test method to distinguish correctly resistant strains = D/C + D; specificity, the capacity [r] ... See full document

... MAC susceptibility testing are not clearly defined or uniformly accepted by all ...which testing might be considered include (i) clinically signif- icant isolates from patients who are receiving or ... See full document

... dilution susceptibility testing is a recognized inexpensive alter- native that can provide DST results within 3 to 4 weeks (8, 11, ...test drug during prolonged ... See full document

... Tuberculosis (TB) poses a grave predicament to the world as it is not merely a scientific challenge but a socio-economic burden as well. A prime cause of mortality in human due to an infectious disease; the malady ... See full document