[PDF] Top 20 Intragenic complementation of herpes simplex virus ICP8 DNA-binding protein mutants.
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Intragenic complementation of herpes simplex virus ICP8 DNA-binding protein mutants.
... This paper reports that the carboxyl-terminal 28 residues of ICP8 are required for a functions involved in viral DNA replication and that the functions missing in nllSV can be supplied i[r] ... See full document
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Genetic analysis of temperature-sensitive mutants which define the gene for the major herpes simplex virus type 1 DNA-binding protein.
... 354-366 0022-538X/83/010354-13$02.00/0 Copyright C 1983, American Society for Microbiology Genetic Analysis of Temperature-Sensitive Mutants Which Define the Gene for the Major Herpes Si[r] ... See full document
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DNA-Binding Activity of Adeno-Associated Virus Rep Is Required for Inverted Terminal Repeat-Dependent Complex Formation with Herpes Simplex Virus ICP8
... by binding to the RBS within the ...cooperativity, ICP8 quickly covers available ssDNA ...the ICP8 domain for interactions with heterologous proteins was described as separate from that engaged in ... See full document
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Identification of a Region of the Herpes Simplex Virus Single-Stranded DNA-Binding Protein Involved in Cooperative Binding
... of ICP8 with 1 mol of FM per mol of protein clearly affects cooperative binding of the ...the protein involved in this impor- tant property. ICP8-FM containing approximately 1 mol of ... See full document
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An immunoassay for the study of DNA-binding activities of herpes simplex virus protein ICP8.
... Vero cells 731 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest An immunoassay was used to examine the interaction between a herpes simplex virus protein, ICP8, and vari[r] ... See full document
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Location, transcript analysis, and partial nucleotide sequence of the cytomegalovirus gene encoding an early DNA-binding protein with similarities to ICP8 of herpes simplex virus type 1.
... 1364 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The results presented here locate the gene encoding an early, nonvirion, single-stranded DNA-binding protein of hum[r] ... See full document
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N-ethylmaleimide inhibition of the DNA-binding activity of the herpes simplex virus type 1 major DNA-binding protein.
... To determine whether this was indeed the case, equilibrium dialysis experiments with ICP8 and [32P]dT1o were done in the presence and absence of NEM as described DISCUSSION The results p[r] ... See full document
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Characterization of herpes simplex virus 2 temperature-sensitive mutants whose lesions map in or near the coding sequences for the major DNA-binding protein.
... In cells infected with certain of these mutants at the nonpermissive temperature, the association of the major DNA-binding protein with the cell nucleus was defective.. In these cells, t[r] ... See full document
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A cytomegalovirus protein with properties of herpes simplex virus ICP8: partial purification of the polypeptide and map position of the gene.
... 3143 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We demonstrated the presence of a single-stranded DNA-binding protein in human cytomegalovirus CMV-infected cells w[r] ... See full document
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Role of the UL25 Protein in Herpes Simplex Virus DNA Encapsidation
... UL25 protein consists of numerous flexible loops extend- ing from a rigid core composed of several tightly packed alpha ...the protein and hence inhibit its function. However, most of the mutants ... See full document
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Phosphorylation of the Herpes Simplex Virus Type 1 Origin Binding Protein
... The herpes simplex virus type 1 (HSV-1) origin binding protein (OBP), the product of the UL9 gene, is one of seven HSV-encoded proteins required for viral DNA ...a DNA ... See full document
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Use of transdominant mutants of the origin-binding protein (UL9) of herpes simplex virus type 1 to define functional domains.
... transdominant mutants provides a genetic ap- proach to map the functional domains of a protein (reviewed in reference ...nant mutants of several HSV-1 proteins, including the trans- activators ICP0, ... See full document
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Interaction with nucleic acids and stimulation of the viral DNA polymerase by the herpes simplex virus type 1 major DNA-binding protein.
... 727 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The interaction of the herpes simplex virus type 1 HSV-1 major DNA-binding protein, infected-cell polypeptide 8 ICP8[r] ... See full document
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Renaturation of complementary DNA strands by herpes simplex virus type 1 ICP8.
... 6945 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest ICP8, the major single-stranded DNA-binding protein of herpes simplex virus type 1, promotes renaturation of complem[r] ... See full document
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Expression of herpes simplex virus type 1 major DNA-binding protein, ICP8, in transformed cell lines: complementation of deletion mutants and inhibition of wild-type virus.
... In addition to cell lines which complemented ts mutants, two lines, U-27 and U-35, significantly inhibited plaque formation by wild-type virus, contained 30 and 100 copies of the ICP8 ge[r] ... See full document
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Characterization of a major DNA-binding domain in the herpes simplex virus type 1 DNA-binding protein (ICP8).
... ICP8, the major DNA-binding protein from herpes simplex virus type 1 HSV-1, belongs to a class of singlestranded DNA-binding proteins.. These proteins are required for DNA replication, p[r] ... See full document
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Genetic evidence for multiple nuclear functions of the herpes simplex virus ICP8 DNA-binding protein.
... 5258 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest We have isolated several mutant herpes simplex viruses, specifically mutated in the infected cell protein 8 ICP8 ge[r] ... See full document
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Correct intranuclear localization of herpes simplex virus DNA polymerase requires the viral ICP8 DNA-binding protein.
... In the absence of viral DNA replication, HSV Pol colocalized with the HSV DNA-binding protein ICP8 in nuclear framework-associated structures called prereplicative sites.. In the presenc[r] ... See full document
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Characterization of the major mRNAs transcribed from the genes for glycoprotein B and DNA-binding protein ICP8 of herpes simplex virus type 1.
... 960 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The structural genes encoding the herpes simplex virus type 1 glycoprotein B and the major DNA-binding protein ICP8 [r] ... See full document
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In vitro characterization of a thermolabile herpes simplex virus DNA-binding protein.
... 31 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The major herpes simplex virus DNA-binding protein, ICP8, was purified from cells infected with the herpes simplex vi[r] ... See full document
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