[PDF] Top 20 KINETIC CONTROL OF NUCLEIC ACID STRAND DISPLACEMENT REACTIONS
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KINETIC CONTROL OF NUCLEIC ACID STRAND DISPLACEMENT REACTIONS
... target nucleic acids in the absence of enzymes and thermal ...mediated strand displacement – a process where one nucleic acid strand binds to a nucleation site on a complementary ... See full document
134
Implementing nonlinear feedback controllers using DNA strand displacement reactions
... feedback control circuits it will be essential to understand the trade- offs between system performance and complexity (particularly in terms of the number of chemical reactions to be imple- mented ... See full document
14
Computational design of nucleic acid feedback control circuits
... DNA strand displacement ...possible reactions between chemical species based on the rules of the extended DSD language, which simpli fi es the design process and identi fi es potential interferences ... See full document
18
Dynamic DNA strand displacement circuits
... Engineered nucleic acid circuits could also be used to modulate gene expression for synthetic biological ...that strand-displacement based reactions networks can be adapted to work ... See full document
290
Implementing nonlinear feedback controllers using DNA strand displacement reactions
... feedback control circuits it will be essential to understand the trade- offs between system performance and complexity (particularly in terms of the number of chemical reactions to be imple- mented ... See full document
14
The concept of RNA-assisted protein folding: the role of tRNA
... amino acid tail into close proximity with the codon-anticodon site (c, d), loses the amino acid, refolds to its original cloverleaf configuration (e) and is ...amino acid pair with its corresponding ... See full document
12
Molecular Beacons of Xeno-Nucleic Acid for Detecting Nucleic Acid
... [62] nucleic acid capture ...electrochemical nucleic acids probe made of PNA which exhibits high sensitivity and specificity when challenged with heterogeneous samples of ... See full document
14
Development of nucleic acid vaccines: use of self-amplifying RNA in lipid nanoparticles
... Nevertheless, these cationic surfactants were also noted to exhibit toxicity. New cationic lipids, which have an aspartate or glutamate backbone, a lysine head-group, and two alkyl tails, yield efficient gene expression ... See full document
11
Translating Ribosomes Inhibit Poliovirus Negative-Strand RNA Synthesis
... in reactions which also contained 2 mM guanidine HCl ...in reactions that contain normal RNA replication com- plexes and guanidine (method 5 in Materials and ... See full document
9
The source of SYBR green master mix determines outcome of nucleic acid amplification reactions
... cell subsets positive for either CD3 (T cells) or CD14 (monocytes). Protocols for total RNA purification were followed as described by the manufacturer. RNA quan- tity was determined on a NanoDrop 2000 Spectropho- ... See full document
10
Real Time Detection of Influenza A, Influenza B, and Respiratory Syncytial Virus A and B in Respiratory Specimens by Use of Nanoparticle Probes
... Patients infected with either influenza virus or RSV can present with similar early symptoms; however, the course of therapy and choice of antiviral agent are different for each virus. Therefore, rapid detection and ... See full document
6
Advances in single molecule nucleic acid sequencing
... system, requiring no prior knowledge of the genome being analyzed. Furthermore, transcriptome quantitation is digital; the abundance of any given transcript is reflected by the number of sequence tags corresponding to ... See full document
164
The evolution of strand preference in simulated RNA replicators with strand displacement: Implications for the origin of transcription
... with strand displacement and show that if the system is well-mixed (under other simplifying assump- tions), there is no selective force acting upon strand preference per ...of strand ... See full document
22
Controls on development and nucleic acid indices in tropical planktonic copepods
... Transformation of the variables and the significance of Levene's test (12 treatments, 100 d.f.) are indicated in the heading. Significant effects are highlighted in bold. If Levene's test was not significant, the level ... See full document
24
On nucleic acid photochemistry
... In order to locate the position of thymine and thymine photodimer on the strip of paper, a strip of paper containing just carrier thymine and thymine dimer was chromatographed adjacent t[r] ... See full document
73
Journal of Applied Pharmaceutical Science
... cells are required to give a positive result; thus some pre-enrichment phase is required. The protocol is generic, however, so the same methodology can be applied to a wide range of microorganisms. The method takes less ... See full document
5
Anoxia-Induced Suspended Animation in Budding Yeast as an Experimental Paradigm for Studying Oxygen-Regulated Gene Expression
... in transcription or translation, along with related metabolic processes. These groups include ribosome and nucleolar com- ponents as well as ribosome biogenesis, RNA metabolism, protein metabolism, amino acid ... See full document
14
Functional nucleic acid probes and uses thereof
... functional nucleic acid probes, and methods of using functional nucleic acid probes, for binding a target to carry out a desired ...functional nucleic acid, at least one ... See full document
16
Zinc Finger Structures in the Human Immunodeficiency Virus Type 1 Nucleocapsid Protein Facilitate Efficient Minus- and Plus-Strand Transfer
... model strand transfer events occurring during virus infection (36, 77) and to compare the activities of wild- type NC and a mutant, termed SSHS NC, having all of the cysteine residues in the zinc fingers changed ... See full document
9
Feline immunodeficiency virus reverse transcriptase: expression, functional characterization, and reconstitution of the 66- and 51-kilodalton subunits.
... sesses strand displacement DNA synthesis activity, each of the two subunits was tested ...form strand displacement DNA synthesis (lane 5), whereas no strand displacement products ... See full document
7
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