[PDF] Top 20 Live-cell imaging with genetically encoded protein kinase activity reporters.

... reported kinase activity reporters has improved them ...for protein kinases, because many peptide sequences that are phosphorylated by a given kinase are not expected to be ... See full document

... Intracellular protein kinases relay information that origi­ nates from diverse chemical and ionic signals received at the cell surface, phosphorylating their substrates to elicit a variety of ...of ... See full document

... novel, genetically encoded, ratiometric pH probe (RaVC) was constructed to image and measure intra- cellular pH in living hyphae of Aspergillus ...chimeric protein based on the pH-sensitive probe ... See full document

... of reporters that are genetically encoded and generate light such as fluorescent proteins and luci- ferases in conjunction with the development of instru- mentation for real time functional ... See full document

... The protein kinase (PK) encoded by the Epstein-Barr Virus (EBV) BGLF4 gene is the only EBV protein ...the protein were analyzed with a polyclonal antiserum raised against a peptide ... See full document

... Finally, although Cakl protein levels and in vitro protein kinase activity do not fluctuate during the cell cycle, at least a fraction of Caklp associates with higher[r] ... See full document

... Three different guanylyl cyclase cell receptors are known, but others will likely be discovered within the next few years. The general function of these receptors appear to relate to the regulation of fluid volume ... See full document

... for cell polarization and formation of branched tubular structures (38, ...efficient cell dissociation, inva- sion of the ECM, and metastasis, whereas isolated acti- vation of either pathway does ... See full document

... B cell is the expression of a BCR and, upon its terminal differ- entiation into a plasma cell, the secretion of highly spe- cific ...and cell proliferation even after transformation of the B cells in ... See full document

... Cry3Aa–DLZM4 were transformed and expressed in Bt as described previously [35]. After isolation of the crys- tals by density gradient centrifugation, pure Cry3Aa- fusion crystals were obtained based on SDS-PAGE analysis ... See full document

... the cell mass), the sample was solubilized in methanol and sub- jected to absorbance measurements at 540 nm using a VersaMax microplate reader (Molecular Devices, Sunny- vale, CA, ... See full document

... and activity increases as cells enter S phase (49, 53), localizing to the ...the cell cycle, but PLK2 is also expressed in the central nervous system – playing a role in synaptic homeostasis and neuronal ... See full document

... cancer cell-selective anticancer drug target Consistent with the important role of Plk1 in regulating various mitotic events, Plk1 overexpression is thought to promote neoplastic transformation of human cells ... See full document

... seed protein content and distinct nutritional benefits thus offering a promising candidate to bridge the gap between increasing demand for high protein and its limiting sources, especially in Asia and ... See full document

... decarboxylase antibodies, and insulin auto-antibodies), autoimmune hepatitis (LKM-2 antibodies), and hypothyroidism (thyroid peroxidase antibodies). In contrast to the patients described by Lohr et al, 9 who had ... See full document

... ATP-binding assay. Immunoprecipitated proteins isolated from 5 ml of VB cell lysate were assayed for ATP binding by immunoblot by using reagents and protocols available from Boehringer Mannheim, Indianapolis, Ind. ... See full document

... in imaging, specifically in bacterial ...113 protein systems are based on proteins that have their active sites modified in such a way that allows them to be covalently modified with a synthetic ... See full document

... to recognize a diverse set of substrates including replication fork intermediates and HJs. The upstream DNA interface of GEN1 lacks the ’acid block’ found in FEN1, instead it has a prominent groove at the same position ... See full document

... the cell population was diploid by FACS ...SPB protein gene NUF2 (KY1344), respectively, were transformed with the same plasmids as in panel A and grown for 6 h in SC-URA medium with 2% ... See full document

... Sdb25-assoeiated kinase com plex and from the fact that Sdb25 shows genetic in ­ teractions w ith Dbf2, the fu n c tio n of w hich is required in late nuclear d ivision (Toyn and Johnston ...Sdb25-associated ... See full document