[PDF] Top 20 Location of the Origin of DNA Replication in Adenovirus Type 2
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Location of the Origin of DNA Replication in Adenovirus Type 2
... If one harvests the heavy side of the Ad2+ND, GC-rich half molecules after cesium chloride equilibrium centrifugation and the light half of the Ad2+ND, AT-rich molecules, it can be shown[r] ... See full document
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OriDB, the DNA replication origin database updated and extended
... Figure 2. Screen shot from S. pombe OriDB showing the Origin Summary Graphic tab for ...the origin of ...the replication origin sites (black for confirmed; dark grey for likely; light ... See full document
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Molecular Architecture of Adenovirus DNA Polymerase and Location of the Protein Primer
... Ad type 2 origin showed protection of the first 20 nucleotides ...on origin-containing DNA and protected bases 9 to 18 (31), but the contribution of each protein is unclear ...core ... See full document
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Selective Modification of Adenovirus Replication Can Be Achieved through Rational Mutagenesis of the Adenovirus Type 5 DNA Polymerase
... 4 Replication of the I664V Ad5 mutant virus is rescued by addition of exogenous dNs to primary ...of 2 independent experiments that yielded similar ... See full document
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High-Mobility Group 1/2 Proteins Are Essential for Initiating Rolling-Circle-Type DNA Replication at a Parvovirus Hairpin Origin
... circle replication, initiation is a com- plex process in which the initiator protein binds to a specific duplex sequence in the replication origin and induces an or- dered series of conformational ... See full document
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A study of the in vitro initiation of adenovirus DNA replication
... Ad 2 helper virus gave around a 20 fold reduction in replication by comparison (Hay, ...the replication origins of Ad2 and Ad4 showed that whilst the 1-18 core domain of the origin is ... See full document
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Adenovirus type 2 DNA replication. II. Termini of DNA replication.
... In our experiments the temporal order of synthesis of different regions of the viral chromosome was studied by an analysis of the distribution of radioactivity in restriction enzyme frag[r] ... See full document
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Insufficient levels of NFIII and its low affinity for the origin of adenovirus type 12 (Ad12) DNA replication contribute to the abortive infection of BHK21 hamster cells by Ad12.
... to DNA or whether lower levels of NFIII exist in these ...the adenovirus- infected cells shows a further increase in NFIII expression, at least not up to 24 ... See full document
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A Hypothesis for DNA Viruses as the Origin of Eukaryotic Replication Proteins
... in DNA Pol alpha by Wang et ...virus type 1 DNA Pol in the study to T4 DNA Pol by Spicer et ...these DNA Pol genes, the sequences are shown as a roughly proportional line drawing in ... See full document
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Mapping and characterization of the origin of DNA replication of porcine circovirus.
... circular DNA genomes. Their mode of replication has been described as rolling-circle-type replication (Table ...the replication origins of all known geminiviruses, a sequence motif with ... See full document
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A DNA structure is required for geminivirus replication origin function.
... Compensatory 2-bp mutations resulting in clustered AT base pairs in the middle of the stem were most detrimental to replication (mutants 5 1 6 and 11 1 ...B replication in the pres- ence of TGMV A ... See full document
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Cloning and characterization of oriL2, a large palindromic DNA replication origin of herpes simplex virus type 2.
... After purification of the insert from pBglO, digestion with Sall lane 5 gave the internal 1,680-bp SalI fragment and, in addition, a small amount of deleted SalI fragment migrating sligh[r] ... See full document
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Adenovirus Type 5 DNA Binding Protein Stimulates Binding of DNA Polymerase to the Replication Origin
... Ad type 5 (Ad5) DBP is a 529-amino-acid (aa) protein with a molecular mass of 59,049 Da, which binds nucleic acids with high ...single-stranded DNA (ssDNA) with a binding site size of ...and ... See full document
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Identification of two distinct regions within the adenovirus minimal origin of replication that are required for adenovirus type 4 DNA replication in vitro.
... Using an in vitro system in which purified fractions or crude extracts of adenovirus type 4-infected HeLa cells can support initiation and elongation on linearized plasmid templates cont[r] ... See full document
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Adenovirus type 2 DNA replication. I. Evidence for discontinuous DNA synthesis.
... Lysis was performed either in 1 volume of 0.5 M NaOH and 10 mM EDTA on top of alkaline sucrose gradients or, if total DNA had to be isolated, through incubation of the nuclear suspension[r] ... See full document
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Intermediate in adenovirus type 2 replication.
... Although intermediate DNA sedimented faster than marker viral DNA in neutral sucrose gradients, single strands longer than unit length could not be detected after alkaline denaturation..[r] ... See full document
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Adenovirus types 2 and 5 functions elicit replication and late expression of adenovirus type 12 DNA in hamster cells.
... In the Adl2-infected transformed cell lines, all or at least most of the Adl2-specific late functions, expressed as mRNA in Adl2 productively infected human cells, appear to be expressed[r] ... See full document
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Interference with simian virus 40 DNA replication by adenovirus type 2 during mixed infection of monkey cells.
... Interference with Simian Virus 40 DNA Replication by Adenovirus Type 2 During Mixed Infection of Monkey Cells FRANS VAN ROY AND WALTER FIERS* Laboratory ofMolecular Biology, State Univer[r] ... See full document
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Mechanisms of adenovirus DNA replication
... Ad.2 DNA replication when compared to plasmids containing only the 1-18 core origin of replication (Hay et ...vitro replication studies by Mul et ...stimulate replication ... See full document
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Termination sites for adenovirus type 2 DNA replication.
... Hybridization of 'H-labeled single strands from heavy DNA with unlabeled L- and H-strand Ad2 DNA Expt Expt % Heavy DNA eluted with 0.14 M phosphate buffer 1 24 2 20 3 4 20 18 aInfected c[r] ... See full document
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