Top PDF Method of producing water-soluble glucuronic acid derivatives of vitamin A

Method of producing water soluble glucuronic acid derivatives of vitamin A

Method of producing water-soluble glucuronic acid derivatives of vitamin A

Method of producing water-soluble glucuronic acid derivatives of vitamin A Abstract Water soluble glucuronic acid derivatives of vitamin A, including retinoyl β-glucuronide and retinyl β[r]

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Estimation of Water-Soluble Vitamin B-Complex in Selected Leafy and Non-Leafy Vegetables by HPLC Method

Estimation of Water-Soluble Vitamin B-Complex in Selected Leafy and Non-Leafy Vegetables by HPLC Method

water soluble vitamin B contents (B 2 , B 3 , B 5 and B 6 ) in five commnly consumed vegetables that were already absent from any published literature. As a continuation of research and given preferences for non-leafy vegetables along with others selected leafy vegetables, the current study was undertaken to determine the vitamin B-complex especially B 1 , B 2 , B 3 , and B 6 using high-performance liquid chromatography (HPLC) with PDA detector. The accuracy and reliability of the HPLC method may provide valuable nutritional information of vitamin B complex in the food matrices of leafy and non-leafy vegetables and also supplement the current Food Composition Table for Bangladesh (FCTB) to calculate the habitual dietary intake and/ or nutritional survey purposes.
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DEVELOPMENT AND VALIDATION OF UV VISIBLE SPECTROMETRIC METHOD FOR ESTIMATION OF WATER SOLUBLE VITAMIN RIBOFLAVIN

DEVELOPMENT AND VALIDATION OF UV VISIBLE SPECTROMETRIC METHOD FOR ESTIMATION OF WATER SOLUBLE VITAMIN RIBOFLAVIN

The present study describes a simple, accurate, precise and cost effective UV-Visible spectrophotometric method for the estimation of Riboflavin raw material. The Riboflavin is water soluble vitamin, so the solvent used throughout the experiment was 0.1N NaOH, the absorption maxima of drug was found at 445 nm. Beer’s law was obeyed in the range of 5ppm-30ppm. the developed method was successfully validated with respect to linearity, accuracy and precision. The method was validated and shown linearity in mentioned concentration. The correlation coefficient for Riboflavin was 0.999. The percentage relative standard deviation of inter-day precision range 0.66-1.04% and intra-day precision 1.05-1.39% both should be less than 2%. Hence proposed method was precise, accurate and cost effective, simple and rapid. This validated method can be applicable for quantitative determination of the titled drug with respect to assay from or for their solid dosage forms.
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Bioanalytical HPLC method of Piper betle L. for quantifying phenolic compound, water-soluble vitamin, and essential oil in five different solvent extracts

Bioanalytical HPLC method of Piper betle L. for quantifying phenolic compound, water-soluble vitamin, and essential oil in five different solvent extracts

The reversed-phase HPLC-DAD was successfully used for identification and quantification of phenolic compound, water-soluble vitamin, and essential oil of Piper betle L. extract in different polarity of solvents. The method demonstrated more compatible with complex compounds involving ascorbic acid, gallic acid, catechin, caffeic acid, syringic acid, rutin, p-coumaric acid, sinapic acid, ferrulic acid, myricetin, quercetin, apigenin, kaempferol, and eugenol, thereby, promising a simple procedure with more efficiency. LC-MS and FTIR synchrotron technique may be considered for further investigation. Consequently, this method could be applied, repeated, and developed for later observation and the present value of Piper betle L. could be recommended for problem solving in pharmaceutical biotechnology community (quality control units), human nutrition, and animal welfare group.
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RP LC METHOD DEVELOPED FOR THE DETERMINATION OF ASCORBIC ACID IN VITAMIN C SYRUP PREPARATION

RP LC METHOD DEVELOPED FOR THE DETERMINATION OF ASCORBIC ACID IN VITAMIN C SYRUP PREPARATION

ABSTRACT: Ascorbic acid is one of the essential and most vital medicines needed to maintain healthy living. Due to its non-availability through endogenous biosynthesis in humans, it is arguably one of the most consumed Over-The-Counter drugs. Thus, a simple, precise, low-cost and accurate reversed-phase liquid chromatographic (RP-LC) method was developed and optimized to analyse for the percentage content of ascorbic acid in different brands of Vitamin C syrups. The chromatographic separation carried out on an ODS (C18) Ultra sphere column, 5µm (25cm x 2mm), was achieved with HPLC water and methanol as the mobile phase, pumped at a flow rate of 1ml/min and using an ultraviolet detector for detection. The calibration curve was linear over the range of 10-100µg/ml and the method was found to be specific as no interference peaks of impurities and excipients were observed. The method developed was used to assess the quality of fifty-five samples of different types of Vitamin C syrup. Among the samples assessed for their percentage content of ascorbic acid, 18.2% fell below B.P official standard, 7.3% fell within range and 74.6% of the total samples analyzed were above the specified range. We have found this method to be simple, rapid and effective for analysis of ascorbic acid in Vitamin C syrups.
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A method for the measurement of vitamin B12 concentration in sea water

A method for the measurement of vitamin B12 concentration in sea water

If there were available a range of organisms each responsive specifically to a different member of the vitamin B12 family of compounds, it would be possible to make separate estimates of[r]

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A suggested method for the assay of vitamin B12 in sea water

A suggested method for the assay of vitamin B12 in sea water

Example Assay All assays of samples of surface water taken in February and March 1954 from Keppel Pier, Millport, on incoming tides, gave results lying between 5 and 10 mfLg B12/l.. A si[r]

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Preparation of Thermosensitive Microcapsules Containing Water Soluble Powder by Melting Dispersion Cooling Method

Preparation of Thermosensitive Microcapsules Containing Water Soluble Powder by Melting Dispersion Cooling Method

The additives added to modify the shell material were α -tocopherol (VE: Pure chem- ical Co. Ltd., Japan) and the oil soluble surfactants which were polygricerine polyrici- norate (PR-300: Riken Vitamin Co. Ltd., Japan) and Soybean Lechitin (SBL: Pure Chemical Co. Ltd., Japan).

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Fabrication and characterization of water soluble vitamin loaded Poly (lactic-co-glycolic acid) aligned electrospun nanofibers

Fabrication and characterization of water soluble vitamin loaded Poly (lactic-co-glycolic acid) aligned electrospun nanofibers

volume ratio that maximizes the chance of cell attachment and growth (Bhutto, Zhang et al. 2016). Aligned fibers have been produced by using different collectors during electrospinning process which not only enhanced the mechanical properties of scaffolds but also increase biological response (Kuihua, Chunyang et al. 2014, Zhang, Qiu et al. 2014, Huang, Kuo et al. 2015, Bhutto, Zhang et al. 2016). PLGA is the most promising polymer used as vehicle for drug delivery and tissue engineering applications(Rowlands, Lim et al. 2007, Lee, Bashur et al. 2009, Li, Wu et al. 2012, Zamani, Latifi et al. 2013).It is FDA approved polymer with diverse properties such as biocompatibility, variable mechanical properties and biodegradability with wide range of erosion time(Holy, Dang et al. 1999, Makadia and Siegel 2011).PLGA is hydrophobic in nature due to presence of methyl group of PLA(Makadia and Siegel 2011). The surface hydrophilicity of the material could be achieved by using surface modification method such as coating with ECM proteins or specific amino acid sequence on the surface (Subramanian, Krishnan et al. 2009). However, physiochemical properties could be enhanced by using some natural micronutrients such as growth factors and other biological molecules in composite manner (He, Xu et al. 2011, Sheng, Fan et al. 2013, Kuihua, Chunyang et al. 2014, Xu, Dong et al. 2014).Vitamins are one of the most important biology micronutrient required to sustain lifeand involved in a variety of reactions such as function as hormones, antioxidants, mediators for cell signalling and as regulators of cells or tissue growth and differentiation(Engelking 2015, Aqeel, Wu et al. 2016).
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Industrial utilizations of water-soluble sulfuric acid lignin prepared by hydrothermal treatment as flocculant and dispersant

Industrial utilizations of water-soluble sulfuric acid lignin prepared by hydrothermal treatment as flocculant and dispersant

Sulfuric acid lignin (SAL) is a byproduct of the acid saccharification of lignocellulosic materials for bio-fuel generation. The recalcitrant and highly complex structure of SAL significantly limits its industrial use, making it a useless indus- trial waste. In a previous study, we proposed a hydrothermal reaction for SAL that can convert it into water-soluble compounds (HSALs), which considerably expanded its industrial use scenario. In this study, we converted this water- soluble HSAL into two ionic modifications with applications as flocculants and dispersants. Firstly, the introduction of a carboxy group was carried out with bromoacetic acid. In the carboxymethylated HSAL (C-HSAL), the carboxymethyl groups content was as high as 3.66 mmol/g. Dispersibility tests using gypsum paste showed that C-HSAL exhibited a dispersibility almost equivalent to that of commercial lignosulfonate. Furthermore, another investigation was con- ducted synthesizing cationized HSAL derivatives by the reaction with glycidyl trimethylammonium chloride (GTA). Dye removal tests were performed with the obtained lignin-based flocculants using three organic dyes. The results showed ideal dye removal ratios for the GTA-HSALs (higher than 90% for all dyes tested), thereby suggesting that they have a huge potential as core molecules to develop high-efficient dye flocculants.
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Epoxidation and etherification of alkaline lignin to prepare water-soluble derivatives and its performance in improvement of enzymatic hydrolysis efficiency

Epoxidation and etherification of alkaline lignin to prepare water-soluble derivatives and its performance in improvement of enzymatic hydrolysis efficiency

Preparation of lignin‑based epoxy resin monomer (LERM) A total of 100 g ECH was put into a three-neck round- bottom flask, and then 10  g AL and 5  g TBAB were added into the ECH at room temperature under mechanical stirring. The obtained mixture was kept at 90  °C for 5  h. Next, 65  mL of 48  % sodium hydroxide aqueous solution was added dropwise into the afore- mentioned mixture at room temperature under vigor- ous stirring, and the reaction was sequentially run for 5 h. After the reaction, the resulting solution was neu- tralized with 50 % acetic acid solution, and the organic layer was washed 4 times with 1 L of distilled water, sep- arated, and dried with anhydrous sodium sulfate. The organic layer was then concentrated to 30 mL at 50 °C under reduced pressure and diluted with 70 mL dichlo- romethane. Furthermore, the diluent was precipitated into petroleum ether, filtered, and dried in ventilation places at room temperature to obtain the lignin-based epoxy resin monomer.
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APPLICATION OF SOME NOVEL TECHNIQUES FOR SOLUBILITY ENHANCEMENT OF MEFENAMIC ACID, A POORLY WATER SOLUBLE DRUG

APPLICATION OF SOME NOVEL TECHNIQUES FOR SOLUBILITY ENHANCEMENT OF MEFENAMIC ACID, A POORLY WATER SOLUBLE DRUG

DSC thermograms of pure drug and corresponding drug carrier system are depicted in Fig. 1. The DSC curve of Mefenamic Acid (MFA-16) shows a sharp endothermic peak (Tpeak = 230.5°C) corresponding to its melting, indicating its crystalline nature. However, the characteristic endothermic peak, corresponding to drug melting was broadened and shifted toward lower temperature, with reduced intensity, in formulations MFA-01, MFA-07, MFA- 012, and MFA-13. This could be attributed to higher polymer concentration and uniform distribution of drug in the crust of polymer, resulting in complete miscibility of molten drug in polymer. No significant difference in DSC pattern of dispersions and physical mixture suggests that the kneading process could not induce interaction at the molecular level and solid dispersion formed is a physical mixture with highly dispersed drug crystals in polymeric carrier system.
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APPLICATIONS OF WATER SOLUBLE POLYMER BLENDS

APPLICATIONS OF WATER SOLUBLE POLYMER BLENDS

Varada Rajulu et al. [85, 86] made an ultrasonic, viscometric, refractometric, density investigation on PVC/PS, PVC/cellulose acetate and cellulose acetate/PMMA blend systems in which these blend combinations were incompatible in nature and PVC/PMMA in cyclohexanone was found to be compatible. C. K. Yeom et al. [87] characterized the sodium alginate (NaAlg) and PVA blend membranes in pervaporation separation by SEM. He et al. [88] developed methods of microscopic observation and macroscopic characterization for determining the co-phase contained in immiscible binary blends. Chen et al. [89] used viscometry method for the miscibility studies of PEO/PVAc blends. Chattopadhyay and Banerjee [90] used visible spectroscopy, polarizing microscope, and FTIR techniques to study the miscibility of polymer blends. Mohamed and Sabaa [91] studied the miscibility and the thermal degradation behaviour of PVC/Poly (vinyl butyral) blend using DSC, TGA and tensile testing methods and reported the blend to be miscible. Elberaich et al. [92] confirmed the miscible nature of PEO/PMMA blend by using optical microscopy and DSC techniques.
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Altered fatty acid, protein, oil, and starch corn lines and method for producing same

Altered fatty acid, protein, oil, and starch corn lines and method for producing same

measurement of protein, oil, and/or starch of seed. In another embodiment, selection is based on differential scanning calorimetry measurement of starch thermal characteristics. In yet another embodiment, selection is based on gas chromatographic measurement of fatty-acid oil composition of seed. In some embodiments, particular types of fatty acids are selected for in the breeding process.

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Removal of glucuronic acid from xylan is a strategy to improve the conversion of plant biomass to sugars for bioenergy

Removal of glucuronic acid from xylan is a strategy to improve the conversion of plant biomass to sugars for bioenergy

In this work, we focussed on xylan, the major hemi- cellulose in secondary cell walls of eudicot angiosperms such as poplar, and an important hemicellulose in coni- fer cell walls, constituting up to 30 and 15% of dry mate- rial, respectively [10]. It is built of a β (1, 4)-linked xylose backbone that carries acetyl and [methyl]glucuronic acid ([Me]GlcA) branches in hardwoods and arabinose and MeGlcA substitutions in softwoods. In Arabidop- sis thaliana, a model for hardwood secondary cell walls, xylan acetylation is believed to be catalysed by several acetyltransferases, of which TBL29/ESK is responsible for transfer of over 50% of acetyl groups [11]. The addi- tion of α-1–2 linked GlcA branches to xylosyl residues is catalysed by GlucUronic acid substitution of Xylan (GUX) enzymes [12, 13]. The average degree of xylan glucuronosylation is 1 in every 8 xylosyl residues in angiosperms [14]. The frequency of [Me]GlcA in gymno- sperms is higher, around 1 in every 6 xylose units [15]. GUX1/GUX2-deficient Arabidopsis thaliana plants (gux1/2) were shown to have no [Me]GlcA decorations on their secondary cell wall xylan [12]. GUX3 is respon- sible for glucuronosylation of xylan in primary walls [16]. GUX1, GUX2, and GUX4 enzymes show activity in vitro [13]. GlcA branches are 4-O-methylated by the
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Amino Acid Conjugation: An Approach to Enhance Aqueous Solubility and Permeability of Poorly Water Soluble Drug Ritonavir

Amino Acid Conjugation: An Approach to Enhance Aqueous Solubility and Permeability of Poorly Water Soluble Drug Ritonavir

Solubility studies were carried out in distilled water. All amino acid conjugates of ritonavir exhibited higher solubility compared to parent drug (Table 1). Serine conjugate of ritonavir showed maximum water solubility. Currently, RT is administered to HIV-1 infected patients in the form of approximately 43% v/v alcoholic solution (NORVIR) as it is practically insoluble in water. Such high alcohol concentrations may not be suitable for pediatric patients. In contrast, amino acid conjugates might offer potential advantages due to their higher solubility relative to RT.
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FAT-SOLUBLE VITAMIN. (Received for publication, September 22, 1925.)

FAT-SOLUBLE VITAMIN. (Received for publication, September 22, 1925.)

Dur next experiments were carried out with goat's milk because we wanted to determine not only the extent to which its anti- rachitic properties could be increased [r]

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Fat-Soluble Vitamin Concentrations in Hypercholesterolemic Children Treated with Colestipol

Fat-Soluble Vitamin Concentrations in Hypercholesterolemic Children Treated with Colestipol

Cholestyramine in the dose of 8 to 12 gm in normal adult human subjects lowered the peak rise in serum vitamin A following an oral load,9' ‘¿Â° but cholestyramine (12 gm/day) did not d[r]

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Efficacy of Fat-Soluble Vitamin Supplementation in Infants With Biliary Atresia

Efficacy of Fat-Soluble Vitamin Supplementation in Infants With Biliary Atresia

Postoperatively, as soon as enteral in- take was tolerated, study participants received 2 mL per os daily of a com- mercially available FSV/TPGS (ADEKs or AquADEKs, trademarks owned by or under license by Axcan Pharma US, Inc, Birmingham, AL). The type of multivi- tamin preparation was changed from ADEKs to AquADEKs ∼ 32 months after the initiation of the study because the manufacturer changed the com- position of this product to the latter formulation. Detailed compositional comparison of ADEKs and AquADEKs is provided in Supplemental Table 4. The TPGS (an amphipathic molecule) po- tentially serves to enhance absorption of FSV that are comixed in these prep- arations. 7 The vitamin composition of
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Synthesis of functional water soluble polymers

Synthesis of functional water soluble polymers

A number of tooth whitening in vitro models evaluating the effects of toothpaste have been reported in the literature, and one of the most commonly used methods for assessing the stain removal is developed by Stookey et al. 84 In this model, square bovine enamel blocks were used and mounted in poly(methyl methacrylate) blocks. The specimens were polished and lightly acid etched in order to facilitate stain accumulation and adherence. Following immersion into a staining media and air drying at 37 o C, the colour of the stained specimens can be measured with a colorimeter. The test toothpastes were dispersed in an aqueous diluent and the stained specimens were then mounted in a mechanical brushing machine and brushed for a set number of brush strokes. The colour of the specimens was remeasured and the amount of stain removed can be calculated. Extracted human teeth have also been used as the substrate in the evaluation of tooth whitening where the toothpaste has been designed to have an effect on the average intrinsic tooth colour. The teeth were first cleaned with a prophylaxis paste to remove any traces of surface extrinsic stain, followed by a brushing protocol with test toothpaste. The changes in intrinsic tooth colour can be measured with a colorimeter, spectrophotometer or by comparison with a Vita shade guide under controlled lighting conditions. 96, 99-101
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