[PDF] Top 20 MicroRNAs as Modulators of Endothelial Differentiation of Stem Cells

... Likewise, endothelial specific deletion of miR-17/92 cluster enhanced col- lateral growth in cardiac and hind limb ischaemia model suggesting a negative role of miR-17/92 cluster in arte- riogenesis ... See full document

... vascular endothelial growth factor (VEGF), insu- lin-like growth factor-1 and basic fibroblast growth factor to produce various protective effects such as promoting vascular regeneration [22], supporting ... See full document

... the differentiation of tyrosine hydroxylase- positive neurons by suppressing Nurr1 ...neural differentiation by interact- ing with NR2F2 ...neural differentiation is illustrated in Additional file ... See full document

... The cells were re- suspended in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and plated in 100-mm culture dishes (Falcon, Oxnard, CA, ... See full document

... derived-stem cells; CD31: Cluster of differentiation 31; CT: Comparative threshold; DMEM: Dulbecco ’ s modified eagle medium; ECs: Endothelial cells; FBS: Fetal bovine serum; FGF: ... See full document

... for differentiation to osteoblasts and characteristics of osteoblasts were deter- mined using MC3T3-E1 ...OB/POBs, cells induced by culture in medium containing hydrocortisone and glycerophosphate, showed a ... See full document

... and differentiation of stem ...in stem cells. Firstly, miRNAs expre- ssed specifically in ES cells have been ...from stem cells (CSCs) and that many cell signaling ... See full document

... directed differentiation protocol to create human induced pluripotent stem cell (hiPSC)- derived endothelial cells (iECs) from patients with fibrodysplasia ossificans progressiva (FOP), a ... See full document

... BMEC differentiation protocols, undifferenti- ated hPSCs were seeded as Versene-dissociated colonies prior to initiating BMEC differentiation [14, ...of differentiation often varied between experi- ... See full document

... placed stem cell research at center stage in cardiology. A stem cell is a cell that has the ability to divide (self replicate) for indefinite periods often throughout the life of the ...with ... See full document

... mesenchymal cells, such as by expressing mesenchymal markers like smooth muscle actin and vimentin ...higher endothelial marker expression and lower mesenchymal marker expression than other sources of ... See full document

... In vitro BBB models have most often been constructed using primary BMECs isolated from rat, bovine, and por- cine sources [16–21]. Such models extended to BMECs in co-culture with astrocytes, pericytes, and neurons, cell ... See full document

... Shi and colleagues also demonstrated that Ang II- stimulated VEGF production by rat BM-MSCs was inhi- bited much more by the AT1R antagonist losartan than the AT2R antagonist PD123319 [33]. Mechanistically, Ang ... See full document

... Protein samples from cultured cells were isolated by using extraction buffer [50 mM Tris (pH 6.8), 20% glycerol, 1% SDS, and a protease inhibitors cocktail (Sigma, St. Louis, MO)]. The protein concentration was ... See full document

... Scratch wound healing assay. The effect of non-cytotoxic levels of BPs (derived from above experiments) on pMSCs migration was assessed and quantified using a scratch wound healing assay. pMSCs were seeded on a 24-well ... See full document

... Mesenchymal stem cells (MSC) obviously alleviate the damage of the structure and function of pul‑ monary vascular endothelial cells ...(ARDSexp). MicroRNAs (miRNAs), as important media ... See full document

... Mesenchymal stem cells (MSC) have become a promising tool for cell therapy in regenerative ...powerful differentiation capabilities and present immunosuppressive properties that aid them in surviving ... See full document

... osteogenic differentiation of rat BMSCs by targeting insulin-like growth factor 1 (IGF1), and promotes fracture healing ...vein endothelial cells (HUVECs) are function- ally connected by connexin 43 ... See full document

... directional differentiation of BMSCs to VECs ...vascular endothelial growth factor (VEGF) family are the strongest known modulators of vascular ...vascular endothelial cells ...vascular ... See full document

... allogeneic stem cells isolated from different tissues [1] is trying to repair damaged ...heart. Stem cells isolated from adipose tissue [2] with high number of pluripotent stromal cells ... See full document