[PDF] Top 20 Minimal sequence requirements of a functional human immunodeficiency virus type 1 primer binding site.
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Minimal sequence requirements of a functional human immunodeficiency virus type 1 primer binding site.
... Transfection of the wild-type proviral genome, pHXB2wild type, into COS-1 cells and subsequent coculture with SupTl cells resulted in the production of infectious virus as measured by p2[r] ... See full document
10
Human immunodeficiency virus type 1 can use different tRNAs as primers for reverse transcription but selectively maintains a primer binding site complementary to tRNA(3Lys).
... predicted sequence of the proviral PBS ...RNA primer that remains following incomplete digestion by the viral RNase H activity at the polypurine tract located just upstream of the U3 region in the viral ... See full document
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Sequence Requirements for Removal of tRNA by an Isolated Human Immunodeficiency Virus Type 1 RNase H Domain
... is sequence or struc- tural recognition by the RNase ...that sequence can influence structure, particularly at a transition junction ...active site interacts with these nucleotides or that these ... See full document
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Minimal Requirement for a Lentivirus Vector Based on Human Immunodeficiency Virus Type 1
... The viral determinants that confer the ability to infect non- dividing cells appear to reside in the p17 matrix (MA) protein (10, 55) and Vpr (21). Viruses with mutations in the MA pro- tein that disrupt the nuclear ... See full document
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Effects of alterations of primer-binding site sequences on human immunodeficiency virus type 1 replication.
... During early stages of infection, the two PBS mutants, in conjunction with tRNA1Ls and tRNAPhe, appar- An early, critical step in the human immunodeficiency virus type HIV-1 life cycle i[r] ... See full document
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Nucleotide substitutions within U5 are critical for efficient reverse transcription of human immunodeficiency virus type 1 with a primer binding site complementary to tRNA(His).
... pHXB2(His-AC-TGT). Virus derived from pHXB2(His-AC-GAC) with M184V RT had slightly delayed replication compared to the virus from pHXB2(His-AC-GAC) with wild-type RT; in contrast, virus from ... See full document
8
Sequence-Specific Binding of Human Immunodeficiency Virus Type 1 Nucleocapsid Protein to Short Oligonucleotides
... the binding of NC to oligodeoxynucleotides containing alternating T’s and G’s, we attempted to identify the minimum length required for stable ...of binding showed that one NC molecule binds stably to every ... See full document
8
Role of CD4 epitopes outside the gp120-binding site during entry of human immunodeficiency virus type 1.
... for human immunodeficiency virus (HIV). The binding site for the surface glycoprotein of HIV type 1 (HIV-1), gp120, has been mapped to the C * -C ( region of domain ... See full document
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Transcription factor binding sites downstream of the human immunodeficiency virus type 1 transcription start site are important for virus infectivity.
... start site (nuc-1) is disrupted during transcriptional activation of the HIV-1 promoter (78, ...(i) sequence- directed nucleosome positioning, (ii) statistical positioning which relies on the ... See full document
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The hydrophobic pocket of cyclophilin is the binding site for the human immunodeficiency virus type 1 Gag polyprotein.
... primary sequence re- quirements of CyP binding to the Gag protein of HIV-1 is evident from the fact that HIV-1 virions depleted of CyPA are not infectious (4, 15, ...CyPA binding (3). ... See full document
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The Human Immunodeficiency Virus Type 1 Gag Polyprotein Has Nucleic Acid Chaperone Activity: Possible Role in Dimerization of Genomic RNA and Placement of tRNA on the Primer Binding Site
... (the primer binding site, or PBS) on the genomic RNA, where it will serve as primer for reverse ...recombinant human immunodeficiency virus type 1 Gag ... See full document
6
Specific binding of human immunodeficiency virus type 1 (HIV-1) Gag-derived proteins to a 5' HIV-1 genomic RNA sequence.
... untranslated sequence into the pSP64poly(A) vector ...PvuII site (position 694 in the HIV-1 sequence), the HindIII site (position 1259), and the PpuMI site (position 1399), ... See full document
5
Leader Sequences Downstream of the Primer Binding Site Are Important for Efficient Replication of Simian Immunodeficiency Virus
... Simian immunodeficiency virus (SIV) and human immuno- deficiency virus type 1 (HIV-1) belong to the primate lentivirus subfamily of ...of human HIV disease ...of ... See full document
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Mutation of the SP1 Sequence Impairs both Multimerization and Membrane-Binding Activities of Human Immunodeficiency Virus Type 1 Gag
... Direct Gag-Gag interactions are mediated mainly by the MA and CA regions. MA can form trimers under certain conditions and thus contains a potential site for Gag-Gag con- tact (22). CA consists of two ... See full document
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Role of Stem B, Loop B, and Nucleotides next to the Primer Binding Site and the Kissing-Loop Domain in Human Immunodeficiency Virus Type 1 Replication and Genomic-RNA Dimerization
... initiation site (DIS) (20, ...klh sequence stimulates genomic RNA dimerization (5, 7, 13, 20), genomic RNA encapsidation (2, 5, 13, 14, 17, 18, 20), and proviral DNA synthesis (18, ...(Fig. 1 [see ... See full document
7
A Functional Role for ADAM10 in Human Immunodeficiency Virus Type 1 Replication
... To determine if ADAM10 is required for proviral DNA integration, genomic and small non-genomic cDNA was isolated from cells at various time points post-infection. If HIV-1 cDNA enters the nucleus but does not ... See full document
14
The human immunodeficiency virus type 1 encapsidation site is a multipartite RNA element composed of functional hairpin structures.
... sense primer 275-298 (5 9 -CCTAGCATTTCGTCACATGGCCCG) and mis- match antisense primer 688-660 MluI (5 9 -CGAGAGATCTCCTCACGCGTT ACTTTCGC) to create an MluI site at nt ...sense primer 275-298 ... See full document
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Inhibition of Human Immunodeficiency Virus Type 1 Replication with Artificial Transcription Factors Targeting the Highly Conserved Primer-Binding Site
... The human immunodeficiency virus type 1 (HIV-1) primer-binding site (PBS) is a highly conserved region in the HIV genome and represents an attractive target ... See full document
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The roles of the human immunodeficiency virus type 1 Pol protein and the primer binding site in the placement of primer tRNA(3Lys) onto viral genomic RNA.
... from primer tRNA) are ...mutated virus BH10-PBS8. The 39 end sequence (AGGGA) of wt PBS in positive-strand DNA is paired with a stretch of homologous nucleotides (TCCCT) downstream of the mutated PBS ... See full document
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Mutations in the U5 Region Adjacent to the Primer Binding Site Affect tRNA Cleavage by Human Immunodeficiency Virus Type 1 Reverse Transcriptase In Vivo
... HIV- 1(ATG) mutant, in which the canonical CA sequence was mutated to AT (Table ...(23). Sequence analysis of the autointegrants produced by infection with the HIV-1(ATG) mutant suggests that ... See full document
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