Top PDF Modulation of Hepatitis C Virus Genome Encapsidation by Nonstructural Protein 4B
Modulation of Hepatitis C Virus Genome Encapsidation by Nonstructural Protein 4B
... stimulating virus genome replication. The impact of the p7 mutation on genome replication was not tested because the replicon contains only the NS3-5B protein-coding ...on virus pro- ...
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Glycine Zipper Motifs in Hepatitis C Virus Nonstructural Protein 4B Are Required for the Establishment of Viral Replication Organelles
... Mutations affecting glycine zipper motifs in NS4B TMS2 and TMS3 disturb subcellular distribution and morphology of HCV-induced double membrane ves- icles, which are reversed by second-site mutations. HCV genome ...
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Protein-Protein Interactions between Hepatitis C Virus Nonstructural Proteins
... Lohmann, R. Bartenschlager, H. E. Blum, K. Bienz, and D. Moradpour, presented at the 9th International Meeting on HCV and Related Viruses, abstr. O-13, p. 28, 2002). These data clearly show that the membranous web, whose ...
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Hepatitis C Virus Nonstructural Protein 5B Is Involved in Virus Morphogenesis
... chimeric genome is comparable to that of the wild type, and the corresponding poly- protein is processed ...infectious virus production could be due to insufficient replication of the interge- ...
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Modulation of Hepatitis C Virus Genome Replication by Glycosphingolipids and Four-Phosphate Adaptor Protein 2
... RNA genome, HCV replicates its RNA on remodeled host membranes composed of lipids hijacked from various internal membrane ...glycosphingolipid-binding protein, is recruited to the HCV replication complex ...
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Modulation of Hepatitis C Virus NS5A Hyperphosphorylation by Nonstructural Proteins NS3, NS4A, and NS4B
... HCV C genome analyzed in this study belongs to genotype 1b and therefore is more closely related to the HCV-J than the HCV-H ...the genome described ...
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Interaction of Hepatitis C Virus Nonstructural Protein 5A with Core Protein Is Critical for the Production of Infectious Virus Particles
... core protein, pre- sumably around ...NS5A-core protein interaction results in disruption of virus production at an early stage of virion ...core protein via interaction with domain III of NS5A ...
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Sequential S232/S235/S238 Phosphorylation of the Hepatitis C Virus Nonstructural Protein 5A
... HCV genome construct (genotype 2a HCV, J6/JFH1) (41) as described previously ...the protein concentrations were quantified. In general, 20 g of protein was separated by SDS-PAGE before the ...
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Virus-Specific Cofactor Requirement and Chimeric Hepatitis C Virus/GB Virus B Nonstructural Protein 3
... Sangar and S. M. Lemon, unpublished results), reminiscent of the 3⬘X element found at the 3⬘ end of HCV genome (35). This will allow the construction of full-length molecular clones of GBV-B (9) as well as ...
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Determinants of the Hepatitis C Virus Nonstructural Protein 2 Protease Domain Required for Production of Infectious Virus
... residue to Phe allowed detectable levels of NS2-3 processing, whereas Lys apparently abolished the function of the active site. Proline 164 has a cis conformation that is thought to bend the peptide backbone to establish ...
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Modulation of the trans-suppression activity of hepatitis C virus core protein by phosphorylation.
... core protein of hepatitis C virus (HCV) can suppress gene expression and replication of hepatitis B virus (HBV) in a human hepatoma cell line ...core protein and examined ...
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Sequence variation within a nonstructural region of the hepatitis G virus genome.
... and protein sequences obtained in this study were compared with HCV ...the C-terminal part of the putative NS4b, the entire NS5a, and almost the complete NS5b ...
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De Novo Initiation of RNA Synthesis by Hepatitis C Virus Nonstructural Protein 5B Polymerase
... RNA genome. Like other positive-strand RNA viruses, replication of hepatitis C virus (HCV) RNA is mediated through a negative-strand intermediate, which is generated through copying the ...
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All Three Domains of the Hepatitis C Virus Nonstructural NS5A Protein Contribute to RNA Binding
... The hepatitis C virus (HCV) nonstructural protein NS5A is critical for viral genome replication and is thought to interact directly with both the RNA-dependent RNA polymerase, ...
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Hepatitis C Virus NS2 Protein Serves as a Scaffold for Virus Assembly by Interacting with both Structural and Nonstructural Proteins
... envelope protein, we inserted an HA epitope tag within the N-terminal region of E1 in the HJ3-5/NS2 YFP ...of virus produc- tion, corresponding to about a 2-log 10 -unit reduction in virus yield ...
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Two distinct proteinase activities required for the processing of a putative nonstructural precursor protein of hepatitis C virus.
... To examine the processing mechanism of the HCV precursor polyprotein, the amino-terminal region of the putative nonstructural protein region of the HCV genome, containing the serine prot[r] ...
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Characterization of a Threonine-Rich Cluster in Hepatitis C Virus Nonstructural Protein 5A and Its Contribution to Hyperphosphorylation
... The reason to use an expression system rather than a replication model in the first place was to allow the analysis of replication-deficient mutants and inhibitors interfer- ing with HCV replication. By comparing NS5A ...
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Nonstructural Protein 5A Impairs DNA Damage Repair: Implications for Hepatitis C Virus-Mediated Hepatocarcinogenesis
... of Hepatitis C Virus, Hallym University, Anyang, South Korea b Ilsong Institute of Life Science, Hallym University, Anyang, South Korea c Korea National Institute of Health, Cheongwon-gun, ...
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Inhibition of Intrahepatic Gamma Interferon Production by Hepatitis C Virus Nonstructural Protein 5A in Transgenic Mice
... viral hepatitis in humans, and the induction of these cytokines in the liver and other tissues of chronically infected patients may have therapeutic value ...HCV nonstructural protein(s) in the ...
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Identification of a Novel Determinant for Membrane Association in Hepatitis C Virus Nonstructural Protein 4B
... 2009 Nonstructural protein 4B (NS4B) plays an essential role in the formation of the hepatitis C virus (HCV) replication ...membrane protein predicted to comprise four ...
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