[PDF] Top 20 Modulation of Translation Initiation Efficiency in Classical Swine Fever Virus

... on virus growth; for example, reduced packaging of viral RNA would result in fewer infectious particles and hence lower growth ...to virus yield and viral RNA ...higher virus yield than both ... See full document

... Steady-state levels of the bicistronic RNAs in vTF7-3-in- fected cells transfected with the indicated plasmids were ana- lyzed to rule out significant differences in these RNAs that could account for the observed ... See full document

... internal initiation have not been defined (10, 11, 22, 25, 26, 28, 34, ...IRES-mediated initiation are also ...in initiation on HCV and CSFV IRESs and to charac- terize their functions in it, we ... See full document

... or translation, since sequencing the template plasmids revealed no mutations which could account for a failure to ...transfection efficiency as reflected by the proportion of NS3-positive cells 16 h after ... See full document

... For classical swine fever virus (CSFV), it was suggested that the synonymous codon usage does not significantly influence virulence, but the relationship between variations in codon pair usage ... See full document

... of translation initiation codon ATG ...a virus stock (P2 stock) which had a titer of 10 ...the virus-expressed CAT protein in a [ 14 C]chloramphenicol assay (26) (data not ... See full document

... Diagnostic Tests CSFV can be detected in blood or tonsil swabs collected from live animals, or in tissue samples (tonsils, pharyngeal and mesenteric lymph nodes, spleen, kidneys, distal ileum) taken at necropsy. Samples ... See full document

... complete virus attenuation in swine. A direct link between reduced virulence in vivo and alteration of the activation profile of immunologically relevant genes could not be de- termined. However, it is ... See full document

... of classical swine fever virus (CSFV) virulence and host range, chimeras of the highly pathogenic Brescia strain and the attenuated vaccine strain CS were constructed and evaluated for viral ... See full document

... higher fever frequency, and higher virus load were observed in 10 4 TCID 50 ...the virus, thus dose effects were not revealed; 3) The differences are not statistically significantly different in most ... See full document

... Received 21 October 2010/Accepted 17 January 2011 Proteolytic processing of polyproteins is considered a crucial step in the life cycle of most positive-strand RNA viruses. An enhancement of NS2-3 processing has been ... See full document

... of classical swine fever virus (CSFV) is a small hydrophobic polypeptide with an apparent molecu- lar mass of 6 to 7 ...for virus growth, demonstrating that CSFV p7 function is critical ... See full document

... related classical swine fever virus (CSFV) strain Brescia clones were isolated from blood samples from an infected ...C1.1.1/CoBrB virus, together with adaptive mutations 276 R, 476 R, ... See full document

... of swine diseases 29–32 and which is banned in the EU (Regulation (EC) ...of swine fevers 33,34 , and the current routine surveillance systems would not (quickly) detect incursions in the non-commercial ... See full document

... - Antibody levels in post-infection sera from a pig inoculated with a low virulent stirain of classical swine fever virus (Hannover 62) and in sera from two pigs inocu[r] ... See full document

... Survival: Moderately fragile and does not persist in the environment. Sensitive to drying and ultraviolet light. Survives well in pens during cold conditions (up to 4 weeks in winter). Survives 3 days at 50°C and 7-15 ... See full document

... The CSFV DI particles are derived from three different origins. Since for all three the same genome structure was found, the possibility of one DI particle contaminating the other virus stocks had to be excluded. ... See full document

... binding of CSFV to cells (11). These findings show that infection with CSFV is highly associated with E rns and E2, which bind with cellular receptors during virus entry. Viruses rely on the host cell to complete ... See full document

... The ability of viruses such as non-cp CSFV to block the induction of IFN not only is important with respect to innate immune reactions against viral infections but also impacts the efficacy of adaptive immune responses, ... See full document

... Apart from above factors that contribute to the patho- genesis and progression of CSF, little is known of changes in serum proteins and biomarker for diagnosis and prognosis of the disease. Recently, growing interest has ... See full document