[PDF] Top 20 Multicenter Evaluation of a New Automated Fourth Generation Human Immunodeficiency Virus Screening Assay with a Sensitive Antigen Detection Module and High Specificity
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Multicenter Evaluation of a New Automated Fourth Generation Human Immunodeficiency Virus Screening Assay with a Sensitive Antigen Detection Module and High Specificity
... RNA detection was performed by using quantitative PCR (Amplicor HIV-1 Monitor; Roche Diagnostics; see Table ...The detection limits of the PCR protocol employed for panels BCP 9010 to 9034 and ANT 6240 to ... See full document
9
Multicenter Evaluation of a New, Automated Enzyme Linked Immunoassay for Detection of Human Immunodeficiency Virus Specific Antibodies and Antigen
... collaborative multicenter study was conducted to evaluate the sensitivity, specificity, and precision of a three-step, fully automated, qualitative microparticle-based enzyme-linked immunoassay ... See full document
9
Evaluation of a New Combined Antigen and Antibody Human Immunodeficiency Virus Screening Assay, VIDAS HIV DUO Ultra
... of human immunodeficiency virus (HIV) infection and laboratory diagnosis, new screening enzyme-linked immu- nosorbent assays which permit the simultaneous detection of HIV ... See full document
7
Improved Detection of Hepatitis B Virus Surface Antigen by a New Rapid Automated Assay
... B virus (HBV) surface antigen (HBsAg) screening assays is continuously improved in order to reduce the residual risk of transfusion-associated hepatitis ...a multicenter study, a new ... See full document
9
Ultra High Throughput, Automated Nucleic Acid Detection of Human Immunodeficiency Virus (HIV) for Infant Infection Diagnosis Using the Gen Probe Aptima HIV 1 Screening Assay
... discriminatory assay. The Procleix discriminatory assay for HIV-1 was used to analyze WB and DBS samples according to the manufacturer’s ...Ultrio assay (5), namely, target capture, TMA, and amplicon ... See full document
5
Enzyme linked immunosorbent assay for detection of equine infectious anemia virus p26 antigen and antibody
... A sensitive specific enzyme-linked immunosorbent assay utilizing purified p26 antigen was developed for the detection of antibodies to equine infectious anemia virus in naturally and exp[r] ... See full document
5
Multicenter Evaluation of the New Abbott RealTime Assays for Quantitative Detection of Human Immunodeficiency Virus Type 1 and Hepatitis C Virus RNA
... Hepatitis C virus (HCV) and human immunodeficiency virus type 1 (HIV-1) are major causes of mortality in both develop- ing and developed countries. For both viruses, relatively effec- tive ... See full document
6
Development of a sensitive quantitative focal assay for human immunodeficiency virus infectivity.
... To test the efficiency of detection of HIV-infected cells by the FIA in HT4-6C cells, HIV infection was analyzed directly by immunofluorescence of HIV-infected A3.01 cells or PHA blast l[r] ... See full document
10
A Sensitive, Quantitative Assay for Human Immunodeficiency Virus Type 1 Integration
... PCR assay that quantitates HIV-1 long terminal repeat (LTR) sequences using molecular beacon de- tection ...a high-titer, randomly integrated, rep- lication-incompetent HIV-1 construct containing an ... See full document
9
Comparison of Multiplex PCR-ELISA and conventional Multiplex PCR for detection of HIV-1/HCV co-infection
... multiplex-PCR-ELISA assay for the detection of multiplex RT-PCR products in a single tube for simultaneous detection of HIV and HCV as two important viral ... See full document
6
Effects of Mutations in the G Tract of the Human Immunodeficiency Virus Type 1 Polypurine Tract on Virus Replication and RNase H Cleavage
... the specificity of RNase H cleavage in vitro; however, the data are complex (18, 24, 25, 28, ...the specificity of PPT-U3 cleavage by HIV-1 RT was affected by mutations in the 3 ⬘ end of the HIV-1 PPT ... See full document
10
A Study on the Usefulness of Fourth Generation Elisa (P24 Antigen and Antibody) for Early Detection of HIV Infection in the High Risk Group
... Detailed clinical history which included age, gender, sexual orientation, occupational history, marital history, sexual history/ last contact, premarital and extramarital contact, HIV seropositivity in contacts, history ... See full document
164
Detection of Candida dubliniensis in Oropharyngeal Samples from Human Immunodeficiency Virus Infected Patients in North America by Primary CHROMagar Candida Screening and Susceptibility Testing of Isolates
... weekly during therapy and quarterly as surveillance cultures by patients swishing and spitting 10 ml of normal saline to be used for culture (6, 9). One-hundred microliters of swish solution was plated on media with and ... See full document
6
Production of Monoclonal Antibodies Directed against the Microsporidium Enterocytozoon bieneusi
... including human immunodeficiency virus (HIV) protease inhibitors has resulted in a decrease in the number of cases of AIDS-related microsporidiosis (10, ... See full document
6
Evaluation of antiviral drugs and neutralizing antibodies to human immunodeficiency virus by a rapid and sensitive microtiter infection assay
... When evaluating anti-HIV agents with this infection assay, testing should be performed at viral titers and incubation periods sufficient to allow eventual virus breakthrough cytolytic re[r] ... See full document
5
Evaluation of a Multiple Cycle, Recombinant Virus, Growth Competition Assay That Uses Flow Cytometry To Measure Replication Efficiency of Human Immunodeficiency Virus Type 1 in Cell Culture
... competition assay to measure HIV-1 replication efficiency over multiple virus replication cy- cles, using flow cytometry to quantify the relative amounts of reference and test ...the assay are the ... See full document
15
Nonspecific alcoholysis, a novel endonuclease activity of human immunodeficiency virus type 1 and other retroviral integrases.
... Implications. We and others have previously noted nonspe- cific nuclease activity by various IN preparations (4, 10, 22–24, 28, 29, 34, 42); the present report reveals one mechanism underlying this activity, i.e., ... See full document
7
Highly Sensitive Multiplex Assay for Detection of Human Immunodeficiency Virus Type 1 and Hepatitis C Virus RNA
... serologic detection of p24 antigen and antiviral antibodies by enzyme ...and sensitive tests for p24 antigen have been developed and implemented, a window period still exists between infection ... See full document
12
Use of a New Dual Antigen Enzyme Linked Immunosorbent Assay To Detect and Characterize the Human Antibody Response to the Human Immunodeficiency Virus Type 2 Envelope gp125 and gp36 Glycoproteins
... our assay (50 to 200 l versus 1 l) (38, 43, 48). However, several fourth-generation mixed HIV-1/2 assays per- formed poorly with diluted (up to 1:1,000) HIV-2 samples, suggesting that they may ... See full document
5
Quantitative detection of human immunodeficiency virus (HIV) antigen by the Enzymun Test: comparison with alternative assays and nucleic acid sequence based amplification of HIV type 1 RNA
... HIV-Ag assay (Boehringer Mann- heim, Mannheim, Germany) is a one-step sandwich assay for the detection of HIV-1 and HIV-2 antigens in human serum, plasma, and cell culture superna- tants and ... See full document
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