[PDF] Top 20 Multilocus Sequence Typing of Clostridium difficile
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Multilocus Sequence Typing of Clostridium difficile
... PCR ribotyping. All PCR ribotyping of reference isolates and cultured isolates described in the present study was performed at the reference labo- ratory for the C. difficile Ribotyping Network for England and ... See full document
9
Multilocus Variable Number Tandem Repeat Analysis and Multilocus Sequence Typing Reveal Genetic Relationships among Clostridium difficile Isolates Genotyped by Restriction Endonuclease Analysis
... of Clostridium difficile infections have increased worldwide in the past ...C. difficile remains predominantly a health care-associated infection, there may also be an increased incidence of ... See full document
7
Multilocus Sequence Typing Analysis of Human and Animal Clostridium difficile Isolates of Various Toxigenic Types
... A multilocus sequence typing (MLST) scheme was developed to study the genetic relationships and popu- lation structure of 72 Clostridium difficile isolates from various hosts, ... See full document
9
Comparison of Seven Techniques for Typing International Epidemic Strains of Clostridium difficile: Restriction Endonuclease Analysis, Pulsed Field Gel Electrophoresis, PCR Ribotyping, Multilocus Sequence Typing, Multilocus Variable Number Tandem Repeat Analysis, Amplified Fragment Length Polymorphism, and Surface Layer Protein A Gene Sequence Typing
... seven Clostridium difficile typing techniques: multilocus variable-number tandem-repeat analysis (MLVA), amplified fragment length polymorphism (AFLP), surface layer protein A gene ... See full document
7
Defining and Evaluating a Core Genome Multilocus Sequence Typing Scheme for Genome Wide Typing of Clostridium difficile
... gene sequence identity and 100% gene sequence overlap) reflecting the high diversity within ...a sequence identity of ⱖ 80% and 100% overlap (with the default parameter stop codon percentage filter ... See full document
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Hash Based Core Genome Multilocus Sequence Typing for Clostridium difficile
... Hash-cgMLST. Using the cgMLST scheme of Bletz et al. (6), the first allele for each of the 2,270 genes was used to create a BLAST search query. Following previous descriptions (6, 10), BLAST searches for each gene ... See full document
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Relatedness of Human and Animal Clostridium difficile PCR Ribotype 078 Isolates Determined on the Basis of Multilocus Variable Number Tandem Repeat Analysis and Tetracycline Resistance
... for typing international epidemic strains of Clostridium difficile: restriction endonuclease analysis, pulsed-field gel electrophoresis, PCR-ribotyping, multilocus sequence ... See full document
6
Multilocus Sequence Typing Subtypes of Poultry Clostridium perfringens Isolates Demonstrate Disease Niche Partitioning
... 2.635), indicating low recombination rates in the core genomes of the C. perfringens isolates examined (35). This hypothesis is substantiated by the observation that 113 of the 142 isolates (79.6%) partitioned into six ... See full document
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Molecular epidemiology of Clostridium difficile infection in Iranian hospitals
... C. difficile strains globally ...cus Sequence Typing Analysis (MLST) is the unam- biguous ability to compare the results obtained in the different regions via the internal database (accessible at ... See full document
7
<p>Phenotypic and Genotypic Characteristics of <em>Clostridium difficile</em> Isolates in Patients with Type 2 Diabetes in Iran</p>
... Patients and Methods: A total of 104 hospitalized patients with type 2 diabetes and nosocomial diarrhea were included in the current study over a 2-year period (2015 – 2017). C. dif fi cile isolates were characterized by ... See full document
8
Development of a Multilocus Sequence Typing Scheme for Molecular Typing of Mycoplasma pneumoniae
... A multilocus sequence typing (MLST) scheme for ...12 sequence types (STs) re- sulted for 57 ...to multilocus variable-number tandem repeat analysis (MLVA) ...genomic sequence ... See full document
9
Multilocus Sequence Typing Scheme for Enterococcus faecium
... the sequence variations in the seven housekeeping genes used in this study; therefore, these housekeeping genes are assumed to be suitable for a population-genetic ... See full document
10
A Trilocus Sequence Typing Scheme for Hospital Epidemiology and Subspecies Differentiation of an Important Nosocomial Pathogen, Enterococcus faecalis
... trilocus sequence typing (TLST) scheme based on intragenic regions of two antigenic genes, ace and salA (encoding a collagen/laminin adhesin and a cell wall-associated antigen, respec- tively), and a gene ... See full document
7
Multilocus Sequence Typing System forCampylobacter jejuni
... nucleotide sequence-based investigation of the relationships of organisms classified as different Campylobacter species is also warranted given the evidence for genetic ex- change among these ... See full document
10
Multilocus sequence typing (MLST), porA and flaA typing of Campylobacter jejuni isolated from cats attending a veterinary clinic
... selected multilocus sequence typing (MLST) ...into sequence types (ST) which in turn can be grouped into clonal complexes (CC) ...with typing of flaA and porA sequences ... See full document
6
Multilocus Sequence Typing of Pathogenic Candida Species
... Each sequence is compared with the reference database for the ...existing sequence, it is assigned that sequence’s allele number ...new sequence, details are sent to the Web database curator, who ... See full document
10
Multilocus Sequence Typing for Characterization of Staphylococcus pseudintermedius
... organism. Multilocus sequence typing (MLST) has been used extensively to define the population genetic structure of Staphylococcus aureus and other bacterial spe- ...the sequence variation of ... See full document
5
The microbial ecology of Campylobacter jejuni in New Zealand within a spatial temporal framework : a thesis presented in partial fulfilment of requirements for the degree of Doctor of Philosophy in Veterinary Science at Massey University, Manawatu, New Zealand
... The multilocus sequence typing system uses an allelic profile based on loci that are part of the core genome and this has been used for source attribution [99] ... See full document
258
Development of a multilocus sequence typing scheme for the molecular typing of Mycoplasma pneumoniae
... Description of Mycoplasma pneumoniae strains used in this study, their sequence type ST and allelic profile, and their MLVA and P1 types.. Strains isolated from the same patient are indi[r] ... See full document
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Multiplex Strategy for Multilocus Sequence Typing, fla Typing, and Genetic Determination of Antimicrobial Resistance of Campylobacter jejuni and Campylobacter coli Isolates Collected in Switzerland
... Campylobacter typing by applying various, mostly ge- netic, methods is used for this ...flaA typing based on the restriction analysis of PCR-amplified fragments or sequencing of the flagellin-encod- ing ... See full document
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