[PDF] Top 20 A new real time PCR protocol for detection of avian haemosporidians
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A new real time PCR protocol for detection of avian haemosporidians
... identified avian haemosporidian species, although true species richness is unknown due to great genetic diversity and insufficient sampling in highly diverse ...standard PCR. Although effective, these ... See full document
9
A duplex real time PCR assay for the detection and quantification of avian reovirus and Mycoplasma synoviae
... are avian reovirus (ARV) and Mycoplasma synoviae ...these avian pathogens in poultry ...duplex real-time PCR assay for the simultaneous detection and quantification of ARV and ... See full document
9
Development and Application of Real Time PCR for Detection of Subgroup J Avian Leukosis Virus
... Although real-time PCR has been widely used in the detection of many viruses and other pathogenic microorganisms, it has lim- ...run real-time ...TaqMan-based ... See full document
6
The Effect of Various Disinfectants on Detection of Avian Influenza Virus by Real Time RT-PCR
... An avian influenza (AI) real time reverse transcriptase-polymerase chain reaction (RRT-PCR) test was previously shown to be a rapid and sensitive method to identify AI virus- infected birds in ... See full document
6
CFX96 Real-Time PCR Detection System. Real-Time PCR: CFX96 System
... overall protocol run time is the time to reach target temperature, which is determined by the average ramp rate and the time for the sample block to reach thermal ...fast time to target ... See full document
8
Eva Green Real Time Pcr Protocol
... is real time pcr protocol bind dna, reliability and conditions of the number of dna to the ...in real protocol printed on amplicon length for critical readings of symmetrical ... See full document
6
Gene Expression Real Time Pcr Protocol
... expression real pcr protocol that can occur when amplifying a dissociation curve analysis, new thermal and ...expression time pcr primer binding protein interactions between ... See full document
5
Quantitative Real Time PCR Protocol. Stack Lab
... Quantitative Real Time PCR Protocol Stack Lab Overview Real-time quantitative polymerase chain reaction (qPCR) differs from regular PCR by including in the reaction ... See full document
8
A New Real Time PCR Assay for Improved Detection of the Parasite Babesia microti
... Center, New York State Department of Health, Albany New York, USA Babesiosis is an emerging zoonosis with important public health implications, as the incidence of the disease has risen dramati- cally over ... See full document
6
Real Time Quantitative PCR for Detection of Helicobacter pylori
... pylori ureC gene when assayed by LightCycler Q-PCR. The classical protocol for isolating nucleic acid from rela- tively large amounts of tissue (200 mg to 1 g) is the proteinase K-phenol method. For ... See full document
9
Comparison of Droplet Digital PCR to Real Time PCR for Quantitative Detection of Cytomegalovirus
... The clinical import of improved assay characteristics is multi- fold. Present methods for measuring CMV load may show quan- titative variability among laboratories exceeding 4 orders of mag- nitude (7, 8). Quantitative ... See full document
7
Detection of a novel avian influenza A (H7N9) virus in humans by multiplex one step real time RT PCR assay
... a PCR assay. To confirm its sensitivity and specificity, the multiplex real-time RT-PCR assay was compared to the WHO- recommended ...of detection of the H7 and FluA genes, with respect ... See full document
9
Real Time Quantitative PCR Detection of Four Human Bocaviruses
... the new assays pre- sented here can be used with minimal technological require- ...the real-time machine to be capable of detecting the FAM signal; no multiplexing capabil- ity with multiple ... See full document
8
Development of real-time PCR assays for the quantitative detection of herpesviruses
... These were simply added to the 12mL conical before pelleting in RPMI media. Volume of RPMI added to total gargle volume was very minimal. Individual sample assay for KSHV. In this assay, samples from an animal model, ... See full document
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Detection of Herpes Simplex Virus DNA by Real Time PCR
... In the third step, a total of 59 cerebrospinal fluid (CSF) specimens were investigated in a comparative study. All specimens had been collected from patients who were admitted to University Hospital, Frankfurt am Main, ... See full document
5
Multiplexed, Real Time PCR for Quantitative Detection of Human Adenovirus
... the new test to be equivalent in sensitivity, with com- plete agreement of both positive and negative results between the two ...The new assay has the added benefit of providing quantitative ... See full document
6
Real Time PCR Assays for Detection of Bocavirus in Human Specimens
... by real-time PCR for the human RNase P gene to monitor specimen quality and the presence of PCR inhibitors as previously described ...a new sample aliquot was considered definitive ... See full document
5
Detection of Coccidioides Species in Clinical Specimens by Real Time PCR
... a real- time PCR assay for the detection of Coccidioides ...turnaround time includes the time for specimen processing and nucleic acid extraction, using a protocol which ... See full document
6
Multiplex Real Time PCR for Detection of Campylobacter, Salmonella, and Shigella
... multiplex PCR are from highly con- served regions of the genes, a weakness of this or any multiplex assay is that new variants of virulence genes could fail to amplify with the primers ...long time ... See full document
8
Detection of Mycobacterium ulcerans by real-time PCR with improved primers
... results, PCR sensitivity was more signifi- cantly improved by new primers for three Japanese iso- ...the new primer/probe set provided only moderate or no improvement of detection sensitivity, ... See full document
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