[PDF] Top 20 Non-canonical amino acid labeling in proteomics and biotechnology
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Non-canonical amino acid labeling in proteomics and biotechnology
... for biotechnology applications where stoichiometric labeling is desired is that ncAA incorpor- ation efficiency for site-specific protein modification often varies by the incorporation ...improve ... See full document
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Discovery of Aminoacyl-tRNA Synthetase Mutants for the Incorporation of Non Canonical Amino Acids into Proteins
... the labeling to only proteins in certain members of a complex cell ...reactive amino acid that requires an engineered MetRS for incorporation, labeling can be directed to individual cells that ... See full document
205
Synthetic Biology Tools for Targeted Incorporation of Non Canonical Amino Acids into Cellular Proteins
... cell-selective, non-canonical amino acid tagging enables a new approach to the labeling, enrichment and identification of virulence factors secreted by pathogenic ...proteomic ... See full document
269
DNA Sequence Polymorphism of the Lactate Dehydrogenase Gene¬from Iranian Plasmodium vivax and Plasmodium falcipa¬rum Isolates
... pLDH gene from Iranian P. falciparum and P.vivax isolates displayed 98.8-100% homology with 1-3 nucleotide substitutions. This rela- tively stability indicated PvLDH and PfLDH genes can be a good antimalaria target and ... See full document
12
Non-Canonical Amino Acids As Biochemical Probes of Ligand-Gated Ion Channel Structure and Function
... and Bpa are photoreactive, they enable temporal control over the crosslinking reaction. Following crosslinking, a number of techniques can be used to analyze products. On the simplest level, SDS-PAGE allows detection of ... See full document
146
A Practical Graph Isomorphism Algorithm with Vertex Canonical Labeling
... (canonical labeling) of the graph, such that for two graphs G and H, C(G)=C(H) if and only if G and H are ...Vertex Canonical Labeling ... See full document
8
Developing deeper understanding of cooking-induced protein modifications in lamb using a proteomic approach
... heat-induced amino acid residue-level modifications were characterised in the cooking ...aromatic amino acids, didehydro of threonine, acetylation of lysine, non-tryptic N-terminal Gln → ... See full document
187
pH-Dependent Changes in Photoaffinity Labeling Patterns of the H1 Influenza Virus Hemagglutinin by Using an Inhibitor of Viral Fusion
... containing amino acids 84 to ...of amino acids ...this labeling pattern could reflect interaction of the compound with the entire stretch of amino acids but covalent labeling only on ... See full document
10
Amino acid substitutions in a polygalacturonase inhibiting protein (OsPGIP2) increases sheath blight resistance in rice
... Bioinformatic analyses of open reading frames (ORFs), introns, exons, deduced amino acid sequences, molecu- lar weights, and pIs were performed using the Vector NTI Suite 8.0 software package. Gene ontology ... See full document
12
Effect of essential and non essential amino acid Effects of protein free energy supplementation on blood metabolites, insulin and hepatic PEPCK gene expression in growing lambs offered rice straw based diet
... and non-es- sential AA were added at the expense of starch to form diets E and E+N, ...Sulphur amino acids, threonine and tryptophan were used in diet E since they are presumably limiting under main- ... See full document
6
Non canonical auxin signalling : fast and curious
... of non-canonical auxin signalling have been linked to TIR1 and the ...prominent non-canonical pathway is associated with one of the auxin-response factors ...the canonical auxin ... See full document
14
Characterisation of the innate immune response to Campylobacter jejuni colonisation in the chicken intestine
... A non-exclusive, non-transferable licence is hereby granted to those using or reproducing, in whole or in part, the material for valid purposes, providing the copyright owners are acknowledged using the ... See full document
204
Efforts Towards The Total Synthesis Of Aureobasidin L
... 2-hydroxy-3-methylpentanoic acid (2S,3S), which is different from the natural product, which has a ...hydroxy acid may have influenced the ability of the two ends to come together for ...3R-methylpentanoic ... See full document
8
Elucidating the Hippocampal Dopaminergic Subproteome with Novel Bioorthogonal Techniques
... does not require the presence of catalysts and proceeds at room temperature, it can be used for in vivo labeling for the visualization of surface azide-bearing glycans (Baskin et al., 2007) or receptors (Schuman ... See full document
129
Expression profile of Wilms Tumor 1 (WT1) isoforms in undifferentiated and all-trans retinoic acid differentiated neuroblastoma cells
... isoforms [10-13]. In mammals, through the mechanism of alternative splicing many transcripts are generated. To date, UniProt databank (http://www.uniprot.org) lists 13 mRNA variants encoding 13 proteins. Other ... See full document
12
Quantitative, Time Resolved Proteomic Analysis Using Bio Orthogonal Non Canonical Amino Acid Tagging
... 19 between the two methods is that BONCAT physically separates newly synthesized proteins prior to MS analysis, while pSILAC distinguishes and quantifies new proteins informatically.[r] ... See full document
138
Amino Acids Profile of Bee Brood, Soldier Termite, Snout Beetle Larva, Silkworm Larva and Pupa: Nutritional Implications
... freezer. Amino acid analysis was by ion exchange chromatography (IEC) using the Technicon Sequential Multisample (TSM) Amino Acid Analyzer (Technicon Instruments Corporation, New ...an ... See full document
8
Degree of Selective Constraint as an Explanation of the Different Rates of Evolution of Gender-Specific Mitochondrial DNA Lineages in the Mussel Mytilus
... Amino acid Substitution tests: An amino acid site was classi- fied as “conservative” if it was occupied by the same amino acid residue in all non-Mytilus species (Tabl[r] ... See full document
9
The Use of Non-Canonical Amino Acids as a Novel Biocontainment Strategy
... Genetically modified organisms (GMO) are a recent scientific advancement widely used in scientific research and industrial fields due to their improved characteristics. They include, but are not limited to, ... See full document
6
Mapping of phosphorylation sites in polyomavirus large T antigen.
... The amino acid composition of the phosphopeptides as revealed by differential labeling with various amino acids indicated that several phosphopeptides contain overlapping sequences and t[r] ... See full document
12
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